2,2',4,4'-四溴二苯醚(BDE-47)对大型溞(<i>Daphnia magna</i>)的毒性效应

熊勤犁; 史雅娟; 吕永龙; 李秋爽; Gosens Jorrit

doi:10.7524/AJE.1673-5897.20120410001

生态毒理学报

2,2',4,4'-四溴二苯醚(BDE-47)对大型溞(Daphnia magna)的毒性效应

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熊勤犁, 史雅娟, 吕永龙, 李秋爽, Gosens Jorrit. 2,2',4,4'-四溴二苯醚(BDE-47)对大型溞(Daphnia magna)的毒性效应[J]. 生态毒理学报, 2013, 8(1): 97-104. doi: 10.7524/AJE.1673-5897.20120410001

引用本文:

熊勤犁, 史雅娟, 吕永龙, 李秋爽, Gosens Jorrit. 2,2',4,4'-四溴二苯醚(BDE-47)对大型溞(Daphnia magna)的毒性效应[J]. 生态毒理学报, 2013, 8(1): 97-104. doi: 10.7524/AJE.1673-5897.20120410001

Xiong Qinli, Shi Yajuan, Lv Yonglong, Li Qiushuang, Gosens Jorrit. Toxic Effects of 2,2',4,4' -Tetrabromodiphenyl Ether (BDE-47) to Daphnia magna[J]. Asian journal of ecotoxicology, 2013, 8(1): 97-104. doi: 10.7524/AJE.1673-5897.20120410001

Citation:

Xiong Qinli, Shi Yajuan, Lv Yonglong, Li Qiushuang, Gosens Jorrit. Toxic Effects of 2,2',4,4' -Tetrabromodiphenyl Ether (BDE-47) to Daphnia magna[J]. Asian journal of ecotoxicology, 2013, 8(1): 97-104. doi: 10.7524/AJE.1673-5897.20120410001

2,2',4,4'-四溴二苯醚(BDE-47)对大型溞(Daphnia magna)的毒性效应

1. 中国科学院生态环境研究中心城市与区域生态国家重点实验室, 北京 100085;
2. 中国科学院研究生院, 北京 100039

作者简介: 熊勤犁(1986-),男,博士研究生,研究方向为生态毒理效应及区域生态风险评价,Email:qinli.xiong@yahoo.com

基金项目:
国家自然科学基金项目(41271487)

国家国际科技合作专项项目(2012DFA91150)
中图分类号: X171.5

Toxic Effects of 2,2',4,4' -Tetrabromodiphenyl Ether (BDE-47) to Daphnia magna

1. State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;
2. Graduate University of the Chinese Academy of Sciences, Beijing 100039, China
Fund Project:

摘要: 2,2',4,4'-四溴二苯醚(BDE-47)在水体及各种水生生物(鱼类、海洋哺乳动物以及水生无脊椎动物)中被广泛检出,但BDE-47对水生无脊椎动物毒性效应的研究还处于起步阶段。以大型溞(Daphnia magna)为受试生物,通过急性(48 h)和慢性(21 d)毒性暴露实验,考察了BDE-47对大型溞活动抑制率、心率、产仔情况和酶活性等指标的影响。结果显示,BDE-47对大型溞活动抑制率的48 h-EC₅₀为112.5 μg·L^-1;高浓度(> 100 μg·L^-1) BDE-47显著诱导提高大型溞的心率。21 d慢性暴露实验中,8 μg·L^-1处理组中大型溞全部死亡;其他各浓度处理组(0.5、1、2、4 μg·L^-1)中,母溞第1胎产仔时间延后,第1胎子代数量减少,总产仔数量大幅减少,这表明大型溞的繁殖能力受到抑制。BDE-47在一定程度上抑制了母溞胆碱酯酶(ChE)和谷胱甘肽硫转移酶(GST)的活性,大型溞体内的代谢机制没有被诱导,神经活性虽被抑制,但抑制率不高。BDE-47大幅诱导过氧化氢酶(CAT)的活性,并呈现一定的剂量效应关系,相对于ChE和GST,CAT对BDE-47暴露更为敏感,可作为BDE-47对大型溞慢性暴露毒性效应的潜在生物标志物。
- 2,2',4,4'-四溴二苯醚 /
- BDE-47 /
- 大型溞 /
- 心率 /
- 繁殖率 /
- 酶活性
Abstract: 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) are widely detected in water and aquatic organisms (fish, marine mammals and aquatic invertebrates), however, there is still lack of research on its toxic effects on aquatic invertebrates. The acute (48 h) and chronic (21 d) tests were conducted to explore the effects of BDE-47 on Daphnia magna with several endpoints as immobilization rate, heart rate, reproduction rate, and the activities of ChE, GST and CAT. Results showed that the 48 h -EC₅₀ value of BDE-47 on the immobilization of D. magna was 112.5 μg·L^-1. An increase in the heart rate of D. magna was significantly induced by BDE-47 at high concentrations (> 100μg·L^-1). In chronic test (21 d), no survival of D. magna in 8 μg·L^-1 BDE-47 exposure group was observed. In the other BDE-47 exposure groups (0.5, 1, 2, 4 μg·L^-1), the first birth was delayed, and the first litter size as the total number of offspring per maternal D. magna were reduced. It was indicated that the fecundity of D. magna was inhibited by BDE-47. The activities of cholinesterase (ChE) and glutathione-S-transferase (GST) of maternal daphnids were inhibited by BDE-47, impling that the metabolism of D. magna was not induced by BDE-47, while neuro-activity was inhibited by BDE-47 in a relatively low extent. Catalase (CAT) activity of maternal D. magna was significantly induced by BDE-47 in a dose-response manner. CAT was more sensitive to BDE-47 than GST or ChE. The activity of CAT could become a potential biomarker to chronic BDE-47 exposure.
- 2,2',4,4'-tetrabromodiphenyl ether /
- BDE-47 /
- Daphnia magna /
- heart rate /
- reproduction /
- enzyme activity

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Citation:

2,2',4,4'-四溴二苯醚(BDE-47)对大型溞(Daphnia magna)的毒性效应

作者简介: 熊勤犁(1986-),男,博士研究生,研究方向为生态毒理效应及区域生态风险评价,Email:qinli.xiong@yahoo.com
1. 中国科学院生态环境研究中心城市与区域生态国家重点实验室, 北京 100085;

2. 中国科学院研究生院, 北京 100039

收稿日期: 2012-04-10

基金项目:

国家自然科学基金项目(41271487)

国家国际科技合作专项项目(2012DFA91150)

关键词:

摘要: 2,2',4,4'-四溴二苯醚(BDE-47)在水体及各种水生生物(鱼类、海洋哺乳动物以及水生无脊椎动物)中被广泛检出,但BDE-47对水生无脊椎动物毒性效应的研究还处于起步阶段。以大型溞(Daphnia magna)为受试生物,通过急性(48 h)和慢性(21 d)毒性暴露实验,考察了BDE-47对大型溞活动抑制率、心率、产仔情况和酶活性等指标的影响。结果显示,BDE-47对大型溞活动抑制率的48 h-EC₅₀为112.5 μg·L^-1;高浓度(> 100 μg·L^-1) BDE-47显著诱导提高大型溞的心率。21 d慢性暴露实验中,8 μg·L^-1处理组中大型溞全部死亡;其他各浓度处理组(0.5、1、2、4 μg·L^-1)中,母溞第1胎产仔时间延后,第1胎子代数量减少,总产仔数量大幅减少,这表明大型溞的繁殖能力受到抑制。BDE-47在一定程度上抑制了母溞胆碱酯酶(ChE)和谷胱甘肽硫转移酶(GST)的活性,大型溞体内的代谢机制没有被诱导,神经活性虽被抑制,但抑制率不高。BDE-47大幅诱导过氧化氢酶(CAT)的活性,并呈现一定的剂量效应关系,相对于ChE和GST,CAT对BDE-47暴露更为敏感,可作为BDE-47对大型溞慢性暴露毒性效应的潜在生物标志物。

English Abstract

Toxic Effects of 2,2',4,4' -Tetrabromodiphenyl Ether (BDE-47) to Daphnia magna

1. State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;
2. Graduate University of the Chinese Academy of Sciences, Beijing 100039, China

Received Date: 2012-04-10

Fund Project:

Keywords:

Abstract: 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) are widely detected in water and aquatic organisms (fish, marine mammals and aquatic invertebrates), however, there is still lack of research on its toxic effects on aquatic invertebrates. The acute (48 h) and chronic (21 d) tests were conducted to explore the effects of BDE-47 on Daphnia magna with several endpoints as immobilization rate, heart rate, reproduction rate, and the activities of ChE, GST and CAT. Results showed that the 48 h -EC₅₀ value of BDE-47 on the immobilization of D. magna was 112.5 μg·L^-1. An increase in the heart rate of D. magna was significantly induced by BDE-47 at high concentrations (> 100μg·L^-1). In chronic test (21 d), no survival of D. magna in 8 μg·L^-1 BDE-47 exposure group was observed. In the other BDE-47 exposure groups (0.5, 1, 2, 4 μg·L^-1), the first birth was delayed, and the first litter size as the total number of offspring per maternal D. magna were reduced. It was indicated that the fecundity of D. magna was inhibited by BDE-47. The activities of cholinesterase (ChE) and glutathione-S-transferase (GST) of maternal daphnids were inhibited by BDE-47, impling that the metabolism of D. magna was not induced by BDE-47, while neuro-activity was inhibited by BDE-47 in a relatively low extent. Catalase (CAT) activity of maternal D. magna was significantly induced by BDE-47 in a dose-response manner. CAT was more sensitive to BDE-47 than GST or ChE. The activity of CAT could become a potential biomarker to chronic BDE-47 exposure.