栉孔扇贝内参基因稳定性研究

刘颖; 王双耀; 高乔; 安立会; 张鹏; 孙静娴; 姜志强; 郑丙辉

doi:10.7524/AJE.1673-5897.20130304001

生态毒理学报

栉孔扇贝内参基因稳定性研究

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刘颖, 王双耀, 高乔, 安立会, 张鹏, 孙静娴, 姜志强, 郑丙辉. 栉孔扇贝内参基因稳定性研究[J]. 生态毒理学报, 2013, 8(4): 616-622. doi: 10.7524/AJE.1673-5897.20130304001

引用本文:

Liu Ying, Wang Shuangyao, Gao Qiao, An Lihui, Zhang Peng, Sun Jingxian, Jiang Zhiqiang, Zheng Binghui. Study on the Expression Stability of Reference Genes in Chlamys Farreri[J]. Asian journal of ecotoxicology, 2013, 8(4): 616-622. doi: 10.7524/AJE.1673-5897.20130304001

Citation:

Liu Ying, Wang Shuangyao, Gao Qiao, An Lihui, Zhang Peng, Sun Jingxian, Jiang Zhiqiang, Zheng Binghui. Study on the Expression Stability of Reference Genes in Chlamys Farreri[J]. Asian journal of ecotoxicology, 2013, 8(4): 616-622. doi: 10.7524/AJE.1673-5897.20130304001

栉孔扇贝内参基因稳定性研究

1. 大连海洋大学水产与生命学院, 大连 116023;
2. 中国环境科学研究院国家环境保护河口与海岸带重点实验室, 北京 100012

作者简介: 刘颖(1987-),女,硕士,研究方向为水环境毒理学,E-mail:liuyinglmeng@gmail.com

基金项目:
国家自然科学基金(21177117)

国家科技支撑计划(2010BAC69B02)
中图分类号: X171.5

Study on the Expression Stability of Reference Genes in Chlamys Farreri

1. College of Aquaculture and Life Science, Dalian Ocean University, Dalian 116023, China;
2. State Environmental Protection Key Laboratory of Estuarine and Coastal Research, Chinese Research Academy for Environment Sciences, Beijing 100012, China
Fund Project:

摘要: 合适的内参基因对准确定量目标基因表达水平非常重要。利用实时定量聚合酶链式反应(RT-qPCR)分析了不同发育阶段和雌激素暴露条件下栉孔扇贝性腺组织中β-actin、β-TUB、EF-lα、18SrRNA和GAPDH 5个内参基因的表达水平,并利用RefFinder软件对其表达稳定性进行了分析。结果表明:在所考察的内参基因中,EF-lα在栉孔扇贝不同发育阶段和雌激素暴露下的表达均最为稳定,可作为定量目标基因表达水平的内参基因之一。
- 内参基因 /
- 栉孔扇贝 /
- 实时定量PCR (RT-qPCR) /
- RefFinder /
- EF-lα
Abstract: Appropriate reference gene is very important to precisely quantify the target gene expression. In this present study, five reference genes mRNA expressions, includingβ-actin, β-TUB, EF-lα, 18SrRNA and GAPDH in Chlamys farreri gonad, were quantified using real-time quantitative polymerase chain reaction (RT-qPCR). Their expression stability was analyzed online using RefFinder software. The results showed that EF-lαexpression was the most stable among these five reference genes in gonad of different development stages and estrogen exposure, suggesting that EF-lαis one of suitable reference gene for quantifying genes expressions in Chlamys farreri gonad.
- reference genes /
- Chlamys farreri /
- real-time quantitative polymerase chain reaction(RT-qPCR) /
- RefFinder /
- EF-lα

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引用本文:

Citation:

栉孔扇贝内参基因稳定性研究

作者简介: 刘颖(1987-),女,硕士,研究方向为水环境毒理学,E-mail:liuyinglmeng@gmail.com
1. 大连海洋大学水产与生命学院, 大连 116023;

2. 中国环境科学研究院国家环境保护河口与海岸带重点实验室, 北京 100012

收稿日期: 2013-03-04

基金项目:

国家自然科学基金(21177117)

国家科技支撑计划(2010BAC69B02)

关键词:

摘要: 合适的内参基因对准确定量目标基因表达水平非常重要。利用实时定量聚合酶链式反应(RT-qPCR)分析了不同发育阶段和雌激素暴露条件下栉孔扇贝性腺组织中β-actin、β-TUB、EF-lα、18SrRNA和GAPDH 5个内参基因的表达水平,并利用RefFinder软件对其表达稳定性进行了分析。结果表明:在所考察的内参基因中,EF-lα在栉孔扇贝不同发育阶段和雌激素暴露下的表达均最为稳定,可作为定量目标基因表达水平的内参基因之一。

English Abstract

Study on the Expression Stability of Reference Genes in Chlamys Farreri

1. College of Aquaculture and Life Science, Dalian Ocean University, Dalian 116023, China;
2. State Environmental Protection Key Laboratory of Estuarine and Coastal Research, Chinese Research Academy for Environment Sciences, Beijing 100012, China

Received Date: 2013-03-04

Fund Project:

Keywords:

reference genes /
Chlamys farreri /
real-time quantitative polymerase chain reaction(RT-qPCR) /
RefFinder /
EF-lα

Abstract: Appropriate reference gene is very important to precisely quantify the target gene expression. In this present study, five reference genes mRNA expressions, includingβ-actin, β-TUB, EF-lα, 18SrRNA and GAPDH in Chlamys farreri gonad, were quantified using real-time quantitative polymerase chain reaction (RT-qPCR). Their expression stability was analyzed online using RefFinder software. The results showed that EF-lαexpression was the most stable among these five reference genes in gonad of different development stages and estrogen exposure, suggesting that EF-lαis one of suitable reference gene for quantifying genes expressions in Chlamys farreri gonad.