Keywords: 
• triclocarban /
• NRK-52E cell /
• cytotoxicity /
• barrier of kidney

Abstract: Triclocarban (TCC) is a broad-spectrum, lipophilic, antimicrobial additive that is largely applied in a variety of personal care products (PCPs). Now, this chemical has been detected in various environmental matrices including sediments, fishes and water. Because of its potential accumulation capacity and biological toxicity, TCC is now increasingly drawing the attention of the researchers. In this study, the potential renal toxicity was evaluated using NRK-52E cells, and we analyzed cell viability, transepithelial electrical resistance (TER) and the protein levels of junctional adhesion molecule 1 (JAM-1) which is closely related to cellular tight junction. The results showed that irregular colonies occurred after exposing NRK-52E cells to 10 μmol·L^-1 TCC for 48 h, and the cell growth was also significantly inhibited in a dose- and time-dependent manners. Also, the TER and the protein levels of JAM-1 decreased after treatment with 3.57 μmol·L^-1 TCC for 48 h, implying the potential adverse effect of TCC on the barrier function of the kidney. Therefore, this study illustrated the in vitro nephrotoxicity of TCC, and the results can provide evidences for future study on toxicity mechanism of TCC and the possible health assessment on animals.