非致死剂量的四溴双酚A胁迫下蚯蚓的生长和抗氧化防御反应

史雅静; 徐晓宇; 王宇; 韩雪松; 李田春; 王玉荣; 史雅娟

doi:10.7524/AJE.1673-5897.20160923001

生态毒理学报

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史雅静, 徐晓宇, 王宇, 韩雪松, 李田春, 王玉荣, 史雅娟. 非致死剂量的四溴双酚A胁迫下蚯蚓的生长和抗氧化防御反应[J]. 生态毒理学报, 2017, 12(5): 304-312. doi: 10.7524/AJE.1673-5897.20160923001

引用本文:

Shi Yajing, Xu Xiaoyu, Wang Yu, Han Xuesong, Li Tianchun, Wang Yurong, Shi Yajuan. Growth Inhibition and Antioxidant Enzyme Response in Earthworms (Eisenia fetida) Exposed to Tetrabromobisphenol A at Non-lethal Dose[J]. Asian Journal of Ecotoxicology, 2017, 12(5): 304-312. doi: 10.7524/AJE.1673-5897.20160923001

Citation:

Shi Yajing, Xu Xiaoyu, Wang Yu, Han Xuesong, Li Tianchun, Wang Yurong, Shi Yajuan. Growth Inhibition and Antioxidant Enzyme Response in Earthworms (Eisenia fetida) Exposed to Tetrabromobisphenol A at Non-lethal Dose[J]. Asian Journal of Ecotoxicology, 2017, 12(5): 304-312. doi: 10.7524/AJE.1673-5897.20160923001

非致死剂量的四溴双酚A胁迫下蚯蚓的生长和抗氧化防御反应

1. 辽宁科技学院生物医药与化学工程学院辽宁省生物医药与化学工程重点实验室, 本溪 117004;
2. 中国科学院生态环境研究中心城市与区域生态国家重点实验室, 北京 100085

作者简介: 史雅静(1963-),女,硕士,教授,研究方向为生态毒理学,E-mail:921083771@qq.com

基金项目:
国家自然科学基金资助项目(No.41671483)；国家国际科技合作专项(No.2012DFA91150)
中图分类号: X171.5

Growth Inhibition and Antioxidant Enzyme Response in Earthworms (Eisenia fetida) Exposed to Tetrabromobisphenol A at Non-lethal Dose

1. School of Biomedical and Chemical Engineering, Liaoning Institute of Science and Technology, Benxi 117004, China;
2. State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Beijing 100085, China
Fund Project:

摘要: 以自然土壤为介质，考察赤子爱胜蚓(Eisenia fetida)的生长抑制率、蛋白质含量、超氧化物歧化酶(SOD)活性、谷胱甘肽转移酶(GST)活性等指标，进行了急性(14 d)、亚急性(28 d)暴露下四溴双酚A(tetrabromobisphenol A, TBBPA)非致死剂量胁迫对蚯蚓的生长和抗氧化防御效应研究。结果表明：在急性试验中TBBPA对蚯蚓的生长抑制均存在显著的剂量效应关系，而在亚急性暴露期，400 mg·kg^-1时显著抑制蚯蚓的生长(P<0.05)；TBBPA对蚯蚓体内蛋白含量影响，在急性试验中各处理间差异不显著(P=0.712)，进入亚急性期蛋白质含量在400 mg·kg^-1时被显著诱导(P=0.039，P<0.05)；TBBPA对蚯蚓SOD酶活性的影响，在急性试验中50 mg·kg^-1时被显著诱导(P<0.01)，在亚急性试验中400 mg·kg^-1时SOD酶活性显著升高(P<0.05)；TBBPA对蚯蚓GST酶活性影响，在急性试验中400 mg·kg^-1时呈现显著诱导效应(P<0.05)，在亚急性期各处理间差异不显著(P=0.428)。蚯蚓生长抑制率、蛋白质含量、体内各生化酶系对TBBPA暴露的时间效应和剂量效应的敏感性存在不同程度差异，应依据污染暴露指标的有效性和敏感性选择多时间段检测和多指标进行土壤安全评价诊断。
- 四溴双酚A /
- 赤子爱胜蚓 /
- 生长抑制率 /
- 蛋白质含量 /
- SOD /
- GST
Abstract: In this paper, the growth inhibition, contents of protein, activity of antioxidant enzymes (SOD and GST) of earthworms (Eisenia fetida) exposed to TBBPA were measured to identify effects of TBBPA on earthworms. TBBPA was spiked to natural soils at non-lethal dose. The results showed that the growth inhibition rates of all TBBPA dose groups during acute period (0-14 d) were significantly higher than those of controls, while in the sub- acute phase the growth of earthworms was inhibited by TBBPA at 400 mg·kg^-1. The protein content was significantly simulated by TBBPA at 400 mg·kg^-1 after 28 d. TBBPA significantly simulated SOD activity at 50 mg·kg^-1 (14 d) and 400 mg·kg^-1 (28 d). GST activity was simulated only at 400 mg·kg^-1 after 14 d. The sensitivity of the indicators as growth inhibition, protein content, antioxidant enzyme activities varied with the exposure time and dose.
- TBBPA /
- Eisenia fetida /
- growth inhibition /
- contents of protein /
- SOD /
- GST

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非致死剂量的四溴双酚A胁迫下蚯蚓的生长和抗氧化防御反应

作者简介: 史雅静(1963-),女,硕士,教授,研究方向为生态毒理学,E-mail:921083771@qq.com
1. 辽宁科技学院生物医药与化学工程学院辽宁省生物医药与化学工程重点实验室, 本溪 117004;

2. 中国科学院生态环境研究中心城市与区域生态国家重点实验室, 北京 100085

收稿日期: 2016-09-23

基金项目:

国家自然科学基金资助项目(No.41671483)；国家国际科技合作专项(No.2012DFA91150)

关键词:

摘要: 以自然土壤为介质，考察赤子爱胜蚓(Eisenia fetida)的生长抑制率、蛋白质含量、超氧化物歧化酶(SOD)活性、谷胱甘肽转移酶(GST)活性等指标，进行了急性(14 d)、亚急性(28 d)暴露下四溴双酚A(tetrabromobisphenol A, TBBPA)非致死剂量胁迫对蚯蚓的生长和抗氧化防御效应研究。结果表明：在急性试验中TBBPA对蚯蚓的生长抑制均存在显著的剂量效应关系，而在亚急性暴露期，400 mg·kg^-1时显著抑制蚯蚓的生长(P<0.05)；TBBPA对蚯蚓体内蛋白含量影响，在急性试验中各处理间差异不显著(P=0.712)，进入亚急性期蛋白质含量在400 mg·kg^-1时被显著诱导(P=0.039，P<0.05)；TBBPA对蚯蚓SOD酶活性的影响，在急性试验中50 mg·kg^-1时被显著诱导(P<0.01)，在亚急性试验中400 mg·kg^-1时SOD酶活性显著升高(P<0.05)；TBBPA对蚯蚓GST酶活性影响，在急性试验中400 mg·kg^-1时呈现显著诱导效应(P<0.05)，在亚急性期各处理间差异不显著(P=0.428)。蚯蚓生长抑制率、蛋白质含量、体内各生化酶系对TBBPA暴露的时间效应和剂量效应的敏感性存在不同程度差异，应依据污染暴露指标的有效性和敏感性选择多时间段检测和多指标进行土壤安全评价诊断。

English Abstract

Growth Inhibition and Antioxidant Enzyme Response in Earthworms (Eisenia fetida) Exposed to Tetrabromobisphenol A at Non-lethal Dose

1. School of Biomedical and Chemical Engineering, Liaoning Institute of Science and Technology, Benxi 117004, China;
2. State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Beijing 100085, China

Received Date: 2016-09-23

Fund Project:

Keywords:

Abstract: In this paper, the growth inhibition, contents of protein, activity of antioxidant enzymes (SOD and GST) of earthworms (Eisenia fetida) exposed to TBBPA were measured to identify effects of TBBPA on earthworms. TBBPA was spiked to natural soils at non-lethal dose. The results showed that the growth inhibition rates of all TBBPA dose groups during acute period (0-14 d) were significantly higher than those of controls, while in the sub- acute phase the growth of earthworms was inhibited by TBBPA at 400 mg·kg^-1. The protein content was significantly simulated by TBBPA at 400 mg·kg^-1 after 28 d. TBBPA significantly simulated SOD activity at 50 mg·kg^-1 (14 d) and 400 mg·kg^-1 (28 d). GST activity was simulated only at 400 mg·kg^-1 after 14 d. The sensitivity of the indicators as growth inhibition, protein content, antioxidant enzyme activities varied with the exposure time and dose.

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非致死剂量的四溴双酚A胁迫下蚯蚓的生长和抗氧化防御反应

作者简介: 史雅静(1963-),女,硕士,教授,研究方向为生态毒理学,E-mail:921083771@qq.com

Growth Inhibition and Antioxidant Enzyme Response in Earthworms (Eisenia fetida) Exposed to Tetrabromobisphenol A at Non-lethal Dose

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非致死剂量的四溴双酚A胁迫下蚯蚓的生长和抗氧化防御反应

English Abstract

Growth Inhibition and Antioxidant Enzyme Response in Earthworms (Eisenia fetida) Exposed to Tetrabromobisphenol A at Non-lethal Dose

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