苏丹红I对小鼠卵巢的损伤作用及对Caspase-3和Ki-67表达的影响

魏明; 甘露; 龙卫红; 侯进; 邓雅婷; 陈玉龙; 陈丽宏

doi:10.7524/AJE.1673-5897.20161031001

生态毒理学报

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魏明, 甘露, 龙卫红, 侯进, 邓雅婷, 陈玉龙, 陈丽宏. 苏丹红I对小鼠卵巢的损伤作用及对Caspase-3和Ki-67表达的影响[J]. 生态毒理学报, 2017, 12(2): 88-93. doi: 10.7524/AJE.1673-5897.20161031001

引用本文:

Wei Ming, Gan Lu, Long Weihong, Hou Jin, Deng Yating, Chen Yulong, Chen Lihong. Injury and Expression of Caspase-3 and Ki-67 in Ovary of Mice Exposed to SudanⅠ[J]. Asian Journal of Ecotoxicology, 2017, 12(2): 88-93. doi: 10.7524/AJE.1673-5897.20161031001

Citation:

Wei Ming, Gan Lu, Long Weihong, Hou Jin, Deng Yating, Chen Yulong, Chen Lihong. Injury and Expression of Caspase-3 and Ki-67 in Ovary of Mice Exposed to SudanⅠ[J]. Asian Journal of Ecotoxicology, 2017, 12(2): 88-93. doi: 10.7524/AJE.1673-5897.20161031001

苏丹红I对小鼠卵巢的损伤作用及对Caspase-3和Ki-67表达的影响

1. 西安医学院药理毒理学教研室, 西安 710021;
2. 陕西省人民医院妇科, 西安 710068;
3. 西安医学院基础与转化医学研究所, 西安 710021

作者简介: 魏明(1978-),男,博士,研究方向为毒理学,E-mail:weiming@xiyi.edu.cn

基金项目:
陕西省自然科学基础研究项目(2016JM8046，2014JQ2-8055)；西安医学院校级重点建设学科资助项目(2015097)；陕西省卫计委重点项目(2014A)；陕西省教育厅重点科研攻关计划项目(16JS096)；西安医学院2016博士科研启动基金项目(2016DOC22)
中图分类号: X171.5

Injury and Expression of Caspase-3 and Ki-67 in Ovary of Mice Exposed to SudanⅠ

1. Department of Pharmacology and Toxicology of Xi'an Medical University, Xi'an 710021, China;
2. Shaanxi Provincial People's Hospital, Xi'an 710068, China;
3. Institute of Basic and Translational Medicine, Xi'an Medical University, Xi'an 710021, China
Fund Project:

摘要: 苏丹红是一种人工合成偶氮染料，可引起肝脏及泌尿系统等多个脏器的肿瘤，但苏丹红对生殖系统的毒性研究较少。本研究以昆明小鼠为受试对象，探讨苏丹红I对小鼠卵巢组织Caspase-3和Ki-67表达的影响。将24只昆明小鼠随机分为：对照组、低剂量(60 mg·kg^-1)、中剂量(120 mg·kg^-1)、高剂量(240 mg·kg^-1) 4组，6只/组。胃灌4周，4周末处死。HE染色观察各组卵巢组织病理变化，免疫组化法检测卵巢组织Caspase-3和Ki-67表达，Real time PCR检测卵巢组织Caspase-3和Ki-67 mRNA表达，Western blotting检测卵巢组织中Ki-67及Caspase-3蛋白表达。与对照组比较，实验组卵巢中Caspase-3阳性细胞率显著升高(P＜0.05)，而Ki-67阳性细胞率显著降低(P＜0.05)，Real time PCR及Western blotting结果与免疫组化法结果一致。本实验发现苏丹红染毒后颗粒细胞的Caspase-3表达增加，且Caspase-3表达呈剂量依赖性，但Ki-67表达受到抑制。
- 苏丹红I /
- 卵巢 /
- Caspase-3 /
- Ki-67
Abstract: Sudan is a synthetic azo dye and can cause tumors in multiple organs including liver and urinary system, but the research in reproductive system toxicity is rare. The effect of Sudan I on the expression of Caspase-3 and Ki-67 in the ovarian tissue of mice was studied by gastric gavage. The 24 Kunming mice were randomly divided into 4 groups: control group, low dose (60 mg·kg^-1), medium dose (120 mg·kg^-1) and high dose (240 mg·kg^-1) group. Each group contained 6 mice. After gastric gavage for 4 weeks, the pathological changes were observed by HE staining. The expression of Caspase-3 and Ki-67 in ovary was detected by immunohistochemistry and the mRNA expression and protein expression of Ki-67 and Caspase-3 in mouse ovarian tissues were detected by real time PCR and western blotting. Compared with the control group, the rate of Ki-67 positive cells was significantly lower in high concentration group (P＜0.05). The rate of Caspase-3 positive cells was significantly increased (P＜0.05) in high concentration group and the results of PCR and western blotting were consistent with the immunohistochemical results. The Caspase-3 expression of ovarian granulosa cell was increased after SDI exposure, but the Ki-67 expression was inhibited.
- Sudan I /
- ovary /
- Caspase-3 /
- Ki-67

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Citation:

苏丹红I对小鼠卵巢的损伤作用及对Caspase-3和Ki-67表达的影响

作者简介: 魏明(1978-),男,博士,研究方向为毒理学,E-mail:weiming@xiyi.edu.cn
1. 西安医学院药理毒理学教研室, 西安 710021;

2. 陕西省人民医院妇科, 西安 710068;

3. 西安医学院基础与转化医学研究所, 西安 710021

收稿日期: 2016-10-31

基金项目:

陕西省自然科学基础研究项目(2016JM8046，2014JQ2-8055)；西安医学院校级重点建设学科资助项目(2015097)；陕西省卫计委重点项目(2014A)；陕西省教育厅重点科研攻关计划项目(16JS096)；西安医学院2016博士科研启动基金项目(2016DOC22)

关键词:

苏丹红I /
卵巢 /
Caspase-3 /
Ki-67

摘要: 苏丹红是一种人工合成偶氮染料，可引起肝脏及泌尿系统等多个脏器的肿瘤，但苏丹红对生殖系统的毒性研究较少。本研究以昆明小鼠为受试对象，探讨苏丹红I对小鼠卵巢组织Caspase-3和Ki-67表达的影响。将24只昆明小鼠随机分为：对照组、低剂量(60 mg·kg^-1)、中剂量(120 mg·kg^-1)、高剂量(240 mg·kg^-1) 4组，6只/组。胃灌4周，4周末处死。HE染色观察各组卵巢组织病理变化，免疫组化法检测卵巢组织Caspase-3和Ki-67表达，Real time PCR检测卵巢组织Caspase-3和Ki-67 mRNA表达，Western blotting检测卵巢组织中Ki-67及Caspase-3蛋白表达。与对照组比较，实验组卵巢中Caspase-3阳性细胞率显著升高(P＜0.05)，而Ki-67阳性细胞率显著降低(P＜0.05)，Real time PCR及Western blotting结果与免疫组化法结果一致。本实验发现苏丹红染毒后颗粒细胞的Caspase-3表达增加，且Caspase-3表达呈剂量依赖性，但Ki-67表达受到抑制。

English Abstract

Injury and Expression of Caspase-3 and Ki-67 in Ovary of Mice Exposed to SudanⅠ

1. Department of Pharmacology and Toxicology of Xi'an Medical University, Xi'an 710021, China;
2. Shaanxi Provincial People's Hospital, Xi'an 710068, China;
3. Institute of Basic and Translational Medicine, Xi'an Medical University, Xi'an 710021, China

Received Date: 2016-10-31

Fund Project:

Keywords:

Sudan I /
ovary /
Caspase-3 /
Ki-67

Abstract: Sudan is a synthetic azo dye and can cause tumors in multiple organs including liver and urinary system, but the research in reproductive system toxicity is rare. The effect of Sudan I on the expression of Caspase-3 and Ki-67 in the ovarian tissue of mice was studied by gastric gavage. The 24 Kunming mice were randomly divided into 4 groups: control group, low dose (60 mg·kg^-1), medium dose (120 mg·kg^-1) and high dose (240 mg·kg^-1) group. Each group contained 6 mice. After gastric gavage for 4 weeks, the pathological changes were observed by HE staining. The expression of Caspase-3 and Ki-67 in ovary was detected by immunohistochemistry and the mRNA expression and protein expression of Ki-67 and Caspase-3 in mouse ovarian tissues were detected by real time PCR and western blotting. Compared with the control group, the rate of Ki-67 positive cells was significantly lower in high concentration group (P＜0.05). The rate of Caspase-3 positive cells was significantly increased (P＜0.05) in high concentration group and the results of PCR and western blotting were consistent with the immunohistochemical results. The Caspase-3 expression of ovarian granulosa cell was increased after SDI exposure, but the Ki-67 expression was inhibited.