离子液体[Bmim]Cl对HepG2细胞糖代谢的影响
Effect of Ionic Liquid [Bmim]Cl on Glucose Metabolism in HepG2
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摘要: 离子液体是一类广泛用于生产、科研的有机溶剂，其生物毒性已被验证，然而在非致死浓度下离子液体对于生物体的潜在影响还有待研究。为探究离子液体对糖代谢的影响，选取人体肝癌细胞HepG2为受试对象进行体外实验，研究一种常用的咪唑类离子液体1-丁基-3-甲基咪唑氯盐（[Bmim]Cl）对HepG2细胞糖代谢的影响。结果表明，[Bmim]Cl暴露会导致HepG2细胞对胞外葡萄糖的吸收显著降低，减少胞内的糖原含量，同时也会抑制细胞活性诱导细胞凋亡，细胞存活率最高下降57%。RT-qPCR结果表明，[Bmim]Cl暴露后，HepG2细胞中糖原分解的重要基因糖原磷酸化酶（PYGL）的表达上调，而调控糖原合成的重要基因糖原合酶2（GYS2）的基因表达在中、低浓度暴露后受到了抑制。此外，葡萄糖转运、糖酵解、糖异生和TCA循环的相关基因在不同的暴露浓度下也出现了不同程度的变化。综上所述，[Bmim]Cl暴露会影响HepG2细胞对胞外葡萄糖的吸收，影响胞内糖原的合成和分解，促进糖酵解，抑制糖异生。高浓度的[Bmim]Cl暴露会引起HepG2细胞的糖代谢紊乱。Abstract: The effect of the potential environmental pollutant ionic liquid 1-butyl-3-methylimidazolium chloride ([Bmim]Cl) on the glucose metabolism of human liver cancer cells HepG2 was studied by using HepG2 cells as experimental models in this paper. The survival rate of HepG2 cells, extracellular glucose consumption, intracellular glycogen content, as well as the major glycometabolic pathway related genes of glycogen decomposition and anabolism, glycolysis, TCA cycle were investigated after the HepG2 cells were exposed to different concentrations of ionic liquid for 24 h. The results showed that [Bmim]Cl could inhibit the cell activity of HepG2 cells and induce cell apoptosis, lead to a significant decrease in extracellular glucose absorption and intracellular glycogen content. The results of real time quantitative PCR showed that the expression of the glycogen synthesis related gene, i.e., glycogen phosphorylase (PYGL) was up-regulated in HepG2 cells after exposure to [Bmim]Cl, while the expression of glycogen synthase 2 (GYS2) was inhibited after exposure to medium and low concentrations of [Bmim]Cl. In addition, we found that the genes involved in glucose transport, glycolysis, gluconeogenesis and TCA cycling were also changed to varying degrees at different exposure concentrations. Overall, [Bmim]Cl can influence the absorption of extracellular glucose by HepG2 cells, affect the synthesis and decomposition of intracellular glycogen, promote glycolysis, and inhibit gluconeogenesis. Exposure to high concentration of [Bmim]Cl can cause glucose metabolism disorder in HepG2 cells.
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Key words:
- ionic liquid /
- HepG2 cells /
- glucose metabolism
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