消毒剂混合物对蛋白核小球藻的协同作用动态定量表征
Dynamic and Quantitative Characterization of Synergism within Disinfectant Mixtures on Chlorella pyrenoidosa
-
摘要: 随着消毒剂在全球范围内的大量使用，越来越多的消毒剂进入环境中，对环境健康构成了威胁。以3种常见的季铵盐类消毒剂为研究对象，包括双十二烷基二甲基溴化铵(didodecyl dimethyl ammonium bromide, DAB)、苯扎溴铵(benzalkonium bromide, BKB)和度米芬(domiphen bromide, DOM)，采用均匀设计射线法设计消毒剂的三元混合体系，应用基于蛋白核小球藻(Chlorella pyrenoidosa, C. pyrenoidosa)的时间依赖微板毒性分析法(time-dependent microplate toxicity analysis, t-MTA)系统考察了消毒剂及其混合物的毒性效应，采用浓度加和(concentration addition, CA)模型定性评估混合物毒性相互作用，并应用拟合归零法动态定量表征毒性相互作用。结果表明，3种消毒剂对C. pyrenoidosa均具有时间依赖毒性效应，在96 h，3种消毒剂的毒性顺序为DOM>BKB>DAB；时间-浓度-效应三维曲面图适合描述效应在空间浓度分布较为均匀的毒性随时间变化规律，在同一暴露时间，消毒剂三元混合物体系对C. pyrenoidosa具有浓度依赖毒性效应，在同一暴露浓度下，消毒剂三元混合体系对C. pyrenoidosa具有时间依赖毒性效应；依据CA模型，三元混合体系的5条射线在24～96 h均表现出时间依赖协同作用；依据拟合归零法，在24～96 h，随时间的延长，5条射线的最大协同作用强度都在逐渐增强，在96 h，5条射线的最大协同作用强度分别为0.3227、0.3507、0.3105、0.2804和0.2626。与CA模型相比，拟合归零法实现了对毒性相互作用的定量表征，更加清晰地呈现出不同浓度水平的毒性相互作用随时间的动态变化规律，为客观评估环境风险提供方法和数据参考。Abstract: With the widespread use of disinfectants around the world, more and more disinfectants enter the environment, posing a threat to environmental health. Three common quaternary ammonium salt disinfectants, didodecyl dimethyl ammonium bromide (DAB), benzalkonium bromide (BKB) and domiphen bromide (DOM), were selected as the research objects. The toxic effects of single disinfectant and their ternary mixture system designed by the uniform design ray method were systematically investigated by the time-dependent microplate toxicity analysis (t-MTA) based on Chlorella pyrenoidosa (C. pyrenoidosa). The toxicity interaction within mixtures were qualitatively evaluated by the concentration addition (CA) model, and quantitatively and dynamically characterized by a fitting-return-to-zero (FRTZ) method. The results showed that three disinfectants all had time-dependent toxicity on C. pyrenoidosa. At 96 h, the toxicity order of the three disinfectants was DOM > BKB > DAB. The time-concentration- response three-dimensional surface diagram was suitable to describe the toxicity changing of pollutants with relatively uniform effect distribution in the whole concentration space in different time. The toxicity of the ternary disinfectant mixture system had concentration-dependency at the same exposure time and time-dependency at the same exposure concentration. According to CA model, five rays in the ternary mixture system showed time-dependent synergism during 24～96 h. According to the FRTZ method, with the extension of time, the maximum synergistic intensity of five rays gradually increases and the maximum synergistic intensity of five rays were 0.3227, 0.3507, 0.3105, 0.2804 and 0.2626 at 96 h, respectively. Compared with CA model, the FRTZ method realizes the quantitative characterization of the toxicity interaction, and more clearly displays the changing rule of toxicity interaction with time at different concentration levels, which provides a method and data reference for the objective assessment of disinfectants’ environmental risks.
-
-
叶利兰, 甘春娟, 陈垚, 等. 疫情防控期间含氯消毒剂大量使用对水生生物的影响综述 [J]. 环境污染与防治, 2021, 43(5): 644-648 Ye L L, Gan C J, Chen Y, et al. Effect of chlorinated disinfectants usage on aquatic organism during the epidemic control: A review [J]. Environmental Pollution & Control, 2021, 43(5): 644-648 (in Chinese)
Tong C Y, Hu H, Chen G, et al. Chlorine disinfectants promote microbial resistance in Pseudomonas sp. [J]. Environmental Research, 2021, 199: 111296 Watson K, Shaw G, Leusch F D, et al. Chlorine disinfection by-products in wastewater effluent: Bioassay-based assessment of toxicological impact [J]. Water Research, 2012, 46(18): 6069-6083 Guo J, Liao M F, He B S, et al. Impact of the COVID-19 pandemic on household disinfectant consumption behaviors and related environmental concerns: A questionnaire-based survey in China [J]. Journal of Environmental Chemical Engineering, 2021, 9(5): 106168 Panseri S, Chiesa L, Ghisleni G, et al. Persistent organic pollutants in fish: Biomonitoring and cocktail effect with implications for food safety [J]. Food Additives & Contaminants Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment, 2019, 36(4): 601-611 El-Nahhal I Y, El-Nahhal Y. Ecological consequences of COVID-19 outbreak [J]. Journal of Water Science and Engineering, 2020, 1(5): 1-5 Park S, Kim H, Ji K. Developmental toxicity and thyroid endocrine disruption of polyhexamethylene guanidine hydrochloride and humidifier disinfectant in zebrafish larvae [J]. Applied Sciences, 2021, 11(11): 4884 Herron J M, Tomita H, White C C, et al. Benzalkonium chloride disinfectants induce apoptosis, inhibit proliferation, and activate the integrated stress response in a 3-D in vitro model of neurodevelopment [J]. Chemical Research in Toxicology, 2021, 34(5): 1265-1274 陈血建, 郭正洋, 黄建飞, 等. 季铵盐类消毒剂的研究现状[J]. 广东化工, 2019, 46(16): 240-241 Chen X J, Guo Z Y, Huang J F, et al. Research progress on quaternary ammonium salt disinfectant [J]. Guangdong Chemical Industry, 2019, 46(16): 240-241 (in Chinese)
Zhang J, Liu S S, Liu H L, et al. A novel method dependent only on the mixture information (MIM) for evaluating the toxicity of mixture [J]. Environmental Pollution, 2011, 159(7): 1941-1947 Mo L Y, Liu S S, Zhu Y N, et al. Combined toxicity of the mixtures of phenol and aniline derivatives to Vibrio qinghaiensis sp.-Q67 [J]. Bulletin of Environmental Contamination and Toxicology, 2011, 87(4): 473-479 王元, 方开泰. 关于均匀分布与试验设计(数论方法)[J]. 科学通报, 1981, 26(2): 65-70 Huang W Y, Liu F, Liu S S, et al. Predicting mixture toxicity of seven phenolic compounds with similar and dissimilar action mechanisms to Vibrio qinghaiensis sp. nov.Q67 [J]. Ecotoxicology and Environmental Safety, 2011, 74(6): 1600-1606 Ge H L, Liu S S, Zhu X W, et al. Predicting hormetic effects of ionic liquid mixtures on luciferase activity using the concentration addition model [J]. Environmental Science & Technology, 2011, 45(4): 1623-1629 Zhang J, Tao M T, Song C C, et al. Time-dependent synergism of five-component mixture systems of aminoglycoside antibiotics to Vibrio qinghaiensis-Q67 induced by a key component [J]. RSC Advances, 2020, 10(21): 12365-12372 刘树深. 化学混合物毒性评估与预测方法[M]. 北京: 科学出版社, 2017: 104-107 胡小贞, 马祖友, 易文利, 等. 4种不同培养基下铜绿微囊藻和四尾栅藻生长比较[J]. 环境科学研究, 2004, 17(S1): 55-57 Hu X Z, Ma Z Y, Yi W L, et al. Growth of Microcystis aeruginosa and Scendesmus quadricauda in four different mediums [J]. Research of Environmental Sciences, 2004, 17(S1): 55-57 (in Chinese)
Wang L J, Liu S S, Yuan J, et al. Remarkable hormesis induced by 1-ethyl-3-methyl imidazolium tetrafluoroborate on Vibrio qinghaiensis sp.-Q67 [J]. Chemosphere, 2011, 84(10): 1440-1445 Liu S S, Song X Q, Liu H L, et al. Combined photobacterium toxicity of herbicide mixtures containing one insecticide [J]. Chemosphere, 2009, 75(3): 381-388 Zhang J, Liu S S, Dong X Q, et al. Predictability of the time-dependent toxicities of aminoglycoside antibiotic mixtures to Vibrio qinghaiensis sp.-Q67 [J]. RSC Advances, 2015, 5(129): 107076-107082 陈琼, 张瑾, 李小猛, 等. 几种抗生素对蛋白核小球藻的时间毒性微板分析法[J]. 生态毒理学报, 2015, 10(2): 190-197 Chen Q, Zhang J, Li X M, et al. Time-dependent microplate toxicity analysis (T-MTA) of several antibiotics to Chlorella pyrenoidosa [J]. Asian Journal of Ecotoxicology, 2015, 10(2): 190-197 (in Chinese)
Zhang Y H, Liu S S, Liu H L, et al. Evaluation of the combined toxicity of 15 pesticides by uniform design [J]. Pest Management Science, 2010, 66(8): 879-887 Zhou X F, Sang W J, Liu S S, et al. Modeling and prediction for the acute toxicity of pesticide mixtures to the freshwater luminescent bacterium Vibrio qinghaiensis sp.-Q67 [J]. Journal of Environmental Sciences, 2010, 22(3): 433-440 刘树深, 张瑾, 张亚辉, 等. APTox: 化学混合物毒性评估与预测[J]. 化学学报, 2012, 70(14): 1511-1517 Liu S S, Zhang J, Zhang Y H, et al. APTox: Assessment and prediction on toxicity of chemical mixtures [J]. Acta Chimica Sinica, 2012, 70(14): 1511-1517 (in Chinese)
朱祥伟, 刘树深, 葛会林, 等. 剂量-效应关系两种置信区间的比较[J]. 中国环境科学, 2009, 29(2): 113-117 Zhu X W, Liu S S, Ge H L, et al. Comparison between two confidence intervals of dose-response relationships [J]. China Environmental Science, 2009, 29(2): 113-117 (in Chinese)
Liu L, Liu S S, Yu M, et al. Application of the combination index integrated with confidence intervals to study the toxicological interactions of antibiotics and pesticides in Vibrio qinghaiensis sp.-Q67 [J]. Environmental Toxicology and Pharmacology, 2015, 39(1): 447-456 黄子晏, 陶梦婷, 张瑾, 等. 重金属与农药污染物对青海弧菌Q67拮抗作用的定量评估[J]. 环境化学, 2020, 39(9): 2441-2449 Huang Z Y, Tao M T, Zhang J, et al. Quantitative evaluation on the antagonism between heavy mental and pesticide pollutants to Vibrio qinghaiensis sp.-Q67 [J]. Environmental Chemistry, 2020, 39(9): 2441-2449 (in Chinese)
卞志强, 张瑾, 王滔, 等. 氨基甲酸酯类农药对蛋白核小球藻联合毒性作用特点及机制[J]. 生态毒理学报, 2019, 14(4): 150-162 Bian Z Q, Zhang J, Wang T, et al. Time-dependent joint toxicity characteristics and mechanisms of five carbamate pesticides towards Chlorella pyrenoidosa [J]. Asian Journal of Ecotoxicology, 2019, 14(4): 150-162 (in Chinese)
Zhang J, Liu S S, Dou R N, et al. Evaluation on the toxicity of ionic liquid mixture with antagonism and synergism to Vibrio qinghaiensis sp.-Q67 [J]. Chemosphere, 2011, 82(7): 1024-1029 Wang L J, Liu S S, Zhang J, et al. A new effect residual ratio (ERR) method for the validation of the concentration addition and independent action models [J]. Environmental Science and Pollution Research International, 2010, 17(5): 1080-1089 宋崇崇, 陶梦婷, 张瑾, 等. 3种重金属对蛋白核小球藻的联合毒性及机理[J]. 环境科学与技术, 2020, 43(2): 88-95 Song C C, Tao M T, Zhang J, et al. Combined toxicity and the mechanisms of three heavy metals to Chlorella pyrenoidosa [J]. Environmental Science & Technology, 2020, 43(2): 88-95 (in Chinese)
丁婷婷, 董欣琪, 张瑾, 等. 3种氨基糖苷类抗生素对水生生物的时间依赖联合毒性作用比较[J]. 生态毒理学报, 2018, 13(1): 126-137 Ding T T, Dong X Q, Zhang J, et al. Comparison of time-dependent joint toxicity interaction of three aminoglycosides antibiotics between two aquatic organisms [J]. Asian Journal of Ecotoxicology, 2018, 13(1): 126-137 (in Chinese)
张瑾, 董欣琪, 陈敏, 等. 五元氨基甲酸酯类农药混合物体系对青海弧菌的毒性特点[J]. 生态毒理学报, 2017, 12(4): 138-145 Zhang J, Dong X Q, Chen M, et al. Toxicity characteristics of five-carbamate pesticide mixture system towards Vibrio qinghaiensis sp. Q67 [J]. Asian Journal of Ecotoxicology, 2017, 12(4): 138-145 (in Chinese)
Zhang Y H, Liu S S, Song X Q, et al. Prediction for the mixture toxicity of six organophosphorus pesticides to the luminescent bacterium Q67 [J]. Ecotoxicology and Environmental Safety, 2008, 71(3): 880-888 Zhang J, Liu S S. Time-dependent stimulations of 1-alkyl-3-methylimidazolium chloride on redox reactants and antioxidases in Vibrio qinghaiensis sp.-Q67 [J]. Journal of Hazardous Materials, 2015, 283: 568-573 陈敏, 张瑾, 董欣琪, 等. 多元抗生素与重金属混合物对蛋白核小球藻的时间依赖性协同与拮抗作用[J]. 农业环境科学学报, 2018, 37(5): 850-859 Chen M, Zhang J, Dong X Q, et al. Time-dependent synergism and antagonism within multi-component mixtures of heavy metals and antibiotics towards Chlorella pyrenoidosa [J]. Journal of Agro-Environment Science, 2018, 37(5): 850-859 (in Chinese)
-
点击查看大图
计量
- 文章访问数: 2269
- HTML全文浏览数: 2269
- PDF下载数: 113
- 施引文献: 0
下载:
