双酚A促进粪肠球菌中信息素调控质粒pCF10介导的耐药基因接合转移
Bisphenol A Promotes Conjugative Transfer of Antibiotic Resistance Genes Mediated by Pheromone-responsive Plasmid in Enterococcus faecalis
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摘要: 粪肠球菌是一种在自然水体中广泛存在的革兰氏阳性细菌。信息素调控质粒介导的接合转移是造成粪肠球菌耐药基因快速扩散的重要方式。双酚A是一种内分泌干扰物，因其在工业中大量应用造成其在水环境中的广泛分布。本文以信息素调控质粒中比较有代表性的pCF10质粒作为研究对象，研究了双酚A对粪肠球菌中耐药基因接合转移的影响，证实了双酚A可以促进pCF10质粒介导的耐药基因接合转移，且这一结果同双酚A作用浓度和作用时间相关。双酚A影响耐药基因的扩散，是通过促进编码正调控信息素的ccfA基因表达实现的。本文旨在深入理解双酚A影响抗生素抗性基因扩散的环境行为，为耐药基因控制及双酚A环境效应的评估提供理论支持。Abstract: Gram-positive bacterium Enterococcus faecalis (E. faecalis) is widespread in natural water environments. The conjugative transfer mediated by pheromone-responsive plasmid is one important way of the rapid spread of antibiotic resistance genes in E. faecalis. As an endocrine disruptor, bisphenol A (BPA) is present in high levels in the aqueous environment because of its wide use in industry. In this paper, we investigated the effect of BPA on the conjugative transfer of antibiotic resistance genes mediated by a representative pheromone-responsive plasmid pCF10 plasmid in E. faecalis. The results showed that BPA could promote pCF10 plasmid-mediated conjugative transfer of antibiotic resistance genes, and the promotion effect was correlated with concentration and time of BPA action. BPA could affect the spread of antibiotic resistance genes by promoting the expression of the ccfA gene, which encodes a positively regulated pheromone. The study could provide an in-depth understanding of the environmental behavior of BPA in affecting the spread of antibiotic resistance genes, and provide theoretical support for the control of antibiotic resistance genes and the assessment of the environmental effects of BPA.
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