纳米塑料对硫酸铜抑制铜绿微囊藻生长的影响作用
Effects of CuSO4 on Cyanobacterium (Microcystis aeruginosa) in Presence of Nanoplastics
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摘要: 研究了水环境中聚苯乙烯（PS）纳米塑料的共存对应急杀藻剂CuSO4抑制铜绿微囊藻的影响作用。带不同基团3种聚苯乙烯纳米塑料单独处理和CuSO4+纳米塑料共存情况下，通过测定藻密度、叶绿素a（Chl a）含量、丙二醛（MDA）含量以及超氧化物歧化酶（SOD）活性，研究了聚苯乙烯纳米塑料与Cu2+对铜绿微囊藻生长抑制的作用机制。结果表明，PS-COOH、PS、PS-NH2这3种纳米塑料均能缓解CuSO4对藻细胞胁迫。与空白对照组（CK）相比，CuSO4、PS-COOH+CuSO4，PS+CuSO4和PS-NH2+CuSO4暴露后藻密度分别抑制了42%、7%、5%、36%，Chl a含量降低了55%、6%、7%和45%，说明PS-NH2与CuSO4共同暴露对藻细胞生长和叶绿素合成的抑制作用与其他2种纳米塑料更为显著。相应地，PS-COOH、PS这2种纳米塑料显著缓解了CuSO4对藻细胞内MDA含量、SOD活性的胁迫。与空白对照相比，PS-COOH+CuSO4和PS+CuSO4处理组MDA含量和SOD活性分别增加了31%、35%和7%、5%，而CuSO4和PS-NH2+CuSO4处理后MDA含量和SOD活性分别增加了99%、66%和22%、5%。同样的，除了PS-NH2外，其他2种纳米塑料均能显著降低在CuSO4处理蓝藻水华过程中铜绿微囊藻胞外藻毒素（MCs）的释放。以上结果表明，带不同基团PS纳米塑料的共存在不同程度上影响CuSO4的除藻效率。Abstract: In this study, we explored the effects of CuSO4 on a toxigenic strain of Cyanobacterium (Microcystis aeruginosa) in the presence of polystyrene nanoplastics (PS NPs). We investigated the individual impact of three different functionalized PS NPs (PS-COOH, PS, PS-NH2), CuSO4, and their combined effects on cell density, chlorophyll a (Chl a), malondialdehyde (MDA) content and superoxide dismutase (SOD) activity. The results demonstrated that the combination of PS NPs alleviated the toxicity of CuSO4 to Cyanobacterium. Compared with the blank control (CK), after exposure to CuSO4, PS-COOH+CuSO4, PS+CuSO4 and PS-NH2+CuSO4, the cell density of Cyanobacterium was inhibited by 42%, 7%, 5% and 36%, respectively, and the Chl a content was decreased by 55% and 6%, 7% and 45%. The results indicated that the co-exposure of PS-NH2 and CuSO4 had more significant inhibitory effects on Cyanobacterium growth and chlorophyll synthesis than the other two PS NPs. Accordingly, PS-COOH and PS significantly alleviated the toxic stress of CuSO4 on the activities of MDA and SOD in Cyanobacterium. Compared with blank control, the MDA content and SOD activity in Cyanobacterium exposed to PS-COOH+CuSO4 and PS+CuSO4 treatment groups were increased by 31% and 35%, as well as 7% and 5%, respectively. While MDA content and SOD activity in Cyanobacterium exposed in CuSO4 and PS-NH2+CuSO4 treatment groups were increased by 99% and 66%, as well as 22% and 5%, respectively. Similarly, except for PS-NH2, the other two PS NPs can significantly reduce the extracellular microcystins (MCs) release from Microcystis aeruginosa during CuSO4 treatment. Our findings illustrate the importance of taking the nanoplastics into account before CuSO4 is applied to control cyanobacteria bloom.
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Key words:
- polystyrene nanoplastics /
- CuSO4 /
- Cyanobacterium /
- interactive effect
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