白洋淀上游流经城区前后细菌群落结构和功能与环境因子的关联研究
Correlation of Environmental Factors with Bacterial Community Structure and Function of River Water Flowing Through Urban Areas in Upper Reaches of Baiyangdian Lake
-
摘要: 细菌群落作为水生态环境的重要组成部分，其结构和功能研究可为水生态环境污染监测与治理提供技术支撑。通过对流经城区白洋淀上游河流细菌群落的高通量测序和环境因子的监测，分析城市环境因素对白洋淀上游水质的影响，并深入探究细菌群落功能与污染物动态变化的关系。结果表明，城市环境对白洋淀上游生态环境的影响较大，上、中、下游的水质等级差异显著。城区中汇入的各类污染物不仅影响细菌群落结构，使得污染严重流域高丰度的细菌种群减少和细菌群落多样性增加；还与细菌群落的硝酸盐和磷酸盐代谢功能密切相关。同时，河流虽汇集了城区的各类污染物，但下游的细菌群落结构和部分氮、磷代谢功能均有恢复为上游水平的趋势。此外，高丰度的黄杆菌属（Flavobacterium）受各类菌群和污染物的抑制作用较大，但验证其与NO-3-N污染物具有较强的相关性。对白洋淀上游流经城区前后细菌群落结构和功能与环境因子动态变化的关联研究，为白洋淀流域生态环境保护与污染治理提供理论依据。Abstract: Due to the importance of bacterial for aquatic ecological environment, the study of the bacterial community structure and function can provide technical support for water ecological pollution monitoring and controlling. Based on high-throughput sequencing of bacterial communities and monitoring of environmental factors of urban area in the upper reaches of Baiyangdian Lake, the impact of urban environmental factors on water quality was analyzed, and the relationship between the bacterial community structure and dynamic changes of some indicative pollutants was systematically explored. The results indicated that the urban area had a significant impact on the ecological environment, and obvious different water quality levels were observed between the upstream, middle and downstream of an urban area upper reaches of Baiyangdian Lake. The various pollutants from the city not only affected the bacterial community structure, but also reduced the population of high abundance bacterial and increased the diversity of bacterial communities in heavily polluted watersheds. Furthermore, they were also closely related to the nitrate and phosphate metabolism functions of bacterial communities. It was also noticed that the bacterial community structure and some nitrogen and phosphorus metabolism functions in the downstream have shown a trend of returning to that of the upstream, given the various pollutants imposed in the urban area. In addition, the high abundance Flavobacterium was greatly inhibited by various bacterial communities and pollutants, with a strong correlation with NO-3-N pollutants as verified in this research. The study on the correlation between the structure and function of bacterial communities and the dynamic changes of environmental factors before and after flowing through the urban area provides a theoretical basis for ecological environment protection and pollution control in the Baiyangdian basin.
-
-
周留洋.保定市白洋淀上游流域水污染治理研究[D].保定:河北大学,2022:14-20Zhou L Y.Baoding Baiyangdian upstream basin waterpollution control research[D].Baoding:Hebei University,2022:14 -20(in Chinese)
Guan X Y,Ren X Q,Tao Y,et al.Study of the water environment risk assessment of the upper reaches of theBaiyangdian Lake,China[J].Water,2022,14(16):2557 张铁坚.保定府河流域水体污染源解析与治理技术体系研究[D].保定:河北农业大学,2019:14-26Zhang T J.Study on water pollution source apportionmentand treatment technology system in Fuhe River Basin of Baoding[D].Baoding:Hebei Agricultural University,2019:14 -26(in Chinese)
李凤,董晓明,董志新,等.四川盆地典型城市与农村河流微生物群落特征比较分析[J].山地学报,2022,40(1):56-70 Li F,Dong X M,Dong Z X,et al.Contrasting analysis of microbial community characteristics in typical urban andrural rivers of Sichuan Basin,China[J].Mountain Research,2022,40(1):56-70(in Chinese)
Falkowski P G,Fenchel T,Delong E F.The microbial engines that drive Earth's biogeochemical cycles[J].Science,2008,320(5879):1034-1039 Tang X M,Xie G J,Shao K Q,et al.Contrast diversitypatterns and processes of microbial community assemblyin a river-lake continuum across a catchment scale innorthwestern China[J].Environmental Microbiome,2020,15(1):10 Yulandi A,Suwanto A,Waturangi D E,et al.Shotgunmetagenomic analysis reveals new insights into bacterialcommunity profiles in tempeh[J].BMC Research Notes,2020,13(1):562 Liu S W,Yu H,Yu Y H,et al.Ecological stability of microbial communities in Lake Donghu regulated by keystone taxa[J].Ecological Indicators,2022,136:108695 朱胜杰,李子武,夏海锋.COD降解菌剂的构建及在污水处理中的评估[J].环境工程学报,2017,11(9):5029-5034 Zhu S J,Li Z W,Xia H F.Construction of microbial agentfor COD degradation and its evaluate in wastewater treatment[J].Chinese Journal of Environmental Engineering,2017,11(9):5029-5034(in Chinese)
Wang H J,Liu X C,Wang Y L,et al.Spatial and temporal dynamics of microbial community composition andfactors influencing the surface water and sediments of urban rivers[J].Journal of Environmental Sciences (China),2023,124:187-197 Ren Z,Qu X D,Peng W Q,et al.Nutrients drive thestructures of bacterial communities in sediments and surface waters in the river-lake system of Poyang Lake[J].Water,2019,11(5):930 Geng M D,Zhang W Z,Hu T,et al.Eutrophication causes microbial community homogenization via modulatinggeneralist species[J].Water Research,2022,210:118003 Yi Y J,Lin C Q,Wang W J,et al.Habitat and seasonalvariations in bacterial community structure and diversityin sediments of a shallow lake[J].Ecological Indicators,2021,120:106959 Przemieniecki S W,Kosewska A,Ciesielski S,et al.Changes in the gut microbiome and enzymatic profile of Tenebrio molitor larvae biodegrading cellulose,polyethylene and polystyrene waste[J].Environmental Pollution,2020,256:113265 Rognes T,Flouri T,Nichols B,et al.VSEARCH:A versatile open source tool for metagenomics[J].PeerJ,2016,4:e2584 Lavy O,Gophna U,Gefen E,et al.The effect of densitydependent phase on the locust gut bacterial composition[J].Frontiers in Microbiology,2018,9:3020 李鹏洋,安启睿,王新皓,等.辽河四平段流域河流沉积物微生物群落多样性和结构分析[J].环境科学,2022,43(5):2586-2594 Li P Y,An Q R,Wang X H,et al.Analysis on diversityand structure of microbial community in river sediment of Siping section of Liaohe River[J].Environmental Science,2022,43(5):2586-2594(in Chinese)
杨清香,李小庆,于淼成,等.黄河三角洲实验区表层沉积物重金属污染特征及防控策略[J].环境工程学报,2023,17(7):2424-2432 Yang Q X,Li X Q,Yu M C,et al.Characteristics andcontrol strategies of heavy metal pollution in surface sediments of the Yellow River Delta experimental zone[J].Chinese Journal of Environmental Engineering,2023,17(7):2424-2432(in Chinese)
张雨晴,邵克强,胡洋,等.岱海水体及沉积物细菌多样性及群落组成特征[J].湖泊科学,2022,34(6):2070-2082 Zhang Y Q,Shao K Q,Hu Y,et al.Bacterial diversity andcommunity composition in lake water and sediment of Lake Daihai[J].Journal of Lake Sciences,2022,34(6):2070-2082(in Chinese)
Langille M G,Zaneveld J,Caporaso J G,et al.Predictivefunctional profiling of microbial communities using 16SrRNA marker gene sequences[J].Nature Biotechnology,2013,31(9):814-821 谢孟星,钱新,刘彤,等.基于微生物完整性指数的河流健康评价:以无锡市为例[J].环境科学学报,2020,40(3):1112-1120 Xie M X,Qian X,Liu T,et al.River health assessmentbased on M-IBI index:Taking Wuxi as an example[J].Acta Scientiae Circumstantiae,2020,40(3):1112-1120(inChinese)
Sun L,Wang J S,Wu Y Y,et al.Community structureand function of epiphytic bacteria associated with Myriophyllum spicatum in Baiyangdian Lake,China[J].Frontiers in Microbiology,2021,12:705509 田熊长,蒋水华,贾卓,等.基于KH-SVM的鄱阳湖水质评价及变化趋势分析[J].环境工程,2023,41(1):72-78 Tian X C,Jiang S H,Jia Z,et al.Water quality evaluationand change trend analysis of the Poyang Lake based onKH-SVM[J].Environmental Engineering,2023,41(1):72-78(in Chinese)
刘幸春,王洪杰,王亚利,等.府河水体及沉积物细菌群落结构分布特征及其影响因素[J].生态毒理学报,2021,16(5):120-135 Liu X C,Wang H J,Wang Y L,et al.Distribution characteristics and influencing factors of bacteria community structure in water and sediments of Fuhe River[J].AsianJournal of Ecotoxicology,2021,16(5):120-135(in Chinese)
张紫薇,陈召莹,张甜娜,等.基于高通量绝对定量测序解析岗南水库微生物群落的时空分布特征及关键驱动因素[J].环境科学学报,2022,42(2):224-239 Zhang Z W,Chen Z Y,Zhang T N,et al.Spatiotemporalcharacteristics and key driving factors of microbial community evolution based on high-throughput absolutequantification sequencing in the Gangnan Reservoir[J].Acta Scientiae Circumstantiae,2022,42(2):224-239(inChinese)
Serrana J M,Li B,Sumi T,et al.Profiling the microbialcommunity structure and functional diversity of a damregulated river undergoing gravel bar restoration[J].Freshwater Biology,2021,66(11):2170-2184 彭宇科,路俊玲,陈慧萍,等.蓝藻水华形成过程对氮磷转化功能细菌群的影响[J].环境科学,2018,39(11):4938-4945 Peng Y K,Lu J L,Chen H P,et al.Dynamic changes of nitrogen-transforming and phosphorus-accumulating bacteria along with the formation of cyanobacterial blooms[J].Environmental Science,2018,39(11):4938-4945(inChinese)
王革林,于鲁冀,王莉,等.生态修复河流微生物群落组成及影响因素研究[J].环境工程技术学报,2023,13(4):1562-1572 Wang G L,Yu L J,Wang L,et al.Study on the composition and influencing factors of microbial community inecological restoration rivers[J].Journal of EnvironmentalEngineering Technology,2023,13(4):1562-1572(in Chinese)
周石磊,孙悦,张艺冉,等.基于网络分析解析水源水库春季水体反硝化群落演变特征及驱动因素[J].环境科学,2020,41(4):1666-1675 Zhou S L,Sun Y,Zhang Y R,et al.Evolution characteristics and driving factors of denitrification community basedon network analysis in the process of spring thermal layerformation in Zhoucun Reservoir[J].Environmental Science,2020,41(4):1666-1675(in Chinese)
谢秀红,韩阳,黄美玉,等.无动力级联生物滤池对山区村镇生活污水的净化与微生物群落特征[J].应用与环境生物学报,2023,29(4):843-852 Xie X H,Han Y,Huang M Y,et al.Purification effect of anondynamic cascading biofilter on rural domestic sewageand the characteristics of the microbial community[J].Chinese Journal of Applied and Environmental Biology,2023,29(4):843-852(in Chinese)
Cai Y J,Liang J S,Zhang P Y,et al.Review on strategies of close-to-natural wetland restoration and a brief caseplan for a typical wetland in Northern China[J].Chemosphere,2021,285:131534 Locey K J,Lennon J T.Scaling laws predict global microbial diversity[J].Proceedings of the National Academy of Sciences of the United States of America,2016,113(21):5970-5975 李函珂,杨远坤,王彬,等.城市河流内源释放与微生物群落构效关系[J].环境科学与技术,2021,44(3):29-36 Li H K,Yang Y K,Wang B,et al.Structure-activity relationship between microbial community and endogenouspollution in urban river[J].Environmental Science&Technology,2021,44(3):29-36(in Chinese)
璩伟卿,张博美,黄雪,等.基于16S rRNA测序技术的青藏高原河流细菌群落多样性[J].环境科学,2023,44(1):262-271 Qu W Q,Zhang B M,Huang X,et al.Bacterial community and diversity of river ecosystems on the Qinghai-TibetPlateau based on 16S rRNA gene sequencing[J].Environmental Science,2023,44(1):262-271(in Chinese)
Sun W,Xia C Y,Xu M Y,et al.Seasonality affects the diversity and composition of bacterioplankton communitiesin Dongjiang River,a drinking water source of HongKong[J].Frontiers in Microbiology,2017,8:1644 -
点击查看大图
计量
- 文章访问数: 563
- HTML全文浏览数: 563
- PDF下载数: 143
- 施引文献: 0
下载:
