四唑虫酰胺对大型溞的急性毒性和毒理
Acute Toxicity and Toxicology of Tetraniliprole on Daphnia magna
-
摘要: 四唑虫酰胺是新一代双酰胺类杀虫剂，具有广阔应用前景，阐明其环境安全性具有重要意义。在本研究中，评估了四唑虫酰胺对水生生物大型溞的生态毒性效应。研究结果表明，四唑虫酰胺对大型溞具有较高的急性毒性风险，其48 h-LC50值为（0.096±0.010） mg·L-1。在0.005 mg·L-1和0.025 mg·L-1，四唑虫酰胺对大型溞的摄食行为和运动行为具有不同程度的抑制作用。此外，四唑虫酰胺导致大型溞体内超氧化物歧化酶（superoxide dismutase，SOD）活性显著升高，而乙酰胆碱酯酶（acetylcholinesterase，AChE）、谷胱甘肽硫转移酶（glutathione S-transferases，GST）和胰蛋白酶（trypsin）活性显著降低。转录组测序结果显示，随着暴露浓度的增加，四唑虫酰胺在大型溞体内诱导的差异表达基因数量逐渐增多。差异表达基因KEGG富集分析结果显示，不同剂量的四唑虫酰胺均显著影响了大型溞体内与代谢和蛋白质合成功能相关信号通路。关键差异基因筛选和验证结果表明，与抗氧化功能相关基因表达量在各四唑虫酰胺处理组均显著上调，而与解毒、消化和核糖体功能相关基因表达量在各四唑虫酰胺处理组均显著下调。本研究阐明了四唑虫酰胺对大型溞的急性风险，并揭示了亚致死浓度下，四唑虫酰胺对大型溞生理功能的影响，相关结果可为四唑虫酰胺在水环境中的环境安全性提供理论依据。Abstract: Tetraniliprole is a new generation of diamide insecticide, which has broad application prospects, and it is important to clarify its environmental safety. In this study, the toxic effects of tetraniliprole on the aquatic organism Daphnia magna were evaluated. The present results show that tetraniliprole has a high risk of acute toxicity to Daphnia magna, evidenced by a LC50 value of (0.096±0.010) mg·L-1 at 48 h. At concentrations of 0.005 mg·L-1 and 0.025 mg·L-1, tetraniliprole inhibited the feeding behavior and motor behavior of Daphnia magna to varying degrees. Additionally, the activities of superoxide dismutase (SOD) in Daphnia magna were significantly enhanced by tetraniliprole, while the activities of acetylcholinesterase (AChE), glutathione S-transferase (GST) and trypsin were significantly inhibited. Transcriptome sequencing results showed that the number of differentially expressed genes (DEGs) induced by tetraniliprole increased gradually with rising exposure concentrations. The KEGG enrichment analysis showed that the metabolism and protein synthesis related signaling pathways were significantly affected by different doses of tetraniliprole. Screening and verification of key DEGs showed that the expression levels of antioxidant-related genes were significantly up-regulated in each tetraniliprole exposure treatment, while the expression levels of genes associated with detoxification, ribosome function and digestion were significantly down-regulated in each tetraniliprole exposure treatment. This study not only elucidates the acute risk of tetraniliprole to Daphnia magna but also unveils the physiological effects of tetraniliprole on the function of Daphnia magna at sublethal concentrations. The obtained insights can provide theoretical basis for the environmental safety of tetraniliprole in aquatic environment.
-
Key words:
- pesticide /
- diamide insecticides /
- Daphnia magna /
- acute toxicity /
- transcriptome sequencing /
- gene expression /
- risk assessment
-
-
陈鹃,柏亚罗.双酰胺类杀虫剂的研发概况及在中国的登记情况[J].世界农药,2021,43(3):22-34 Chen J,Bai Y L.Overview on R&D of diamide insecticides and their registrations in China[J].World Pesticide,2021,43(3):22-34(in Chinese)
陈小平,柳爱平,关少飞,等.双酰胺类杀虫剂及其应用市场与防治刺吸式口器害虫前景[J].世界农药,2021,43(11):1-12 Chen X P,Liu A P,Guan S F,et al.Diamide insecticidesand their application market and prospect of controllingsucking pests[J].World Pesticide,2021,43(11):1-12(inChinese)
何旭峰,黄小兰,肖琦,等.QuEChERS-超高效液相色谱-串联质谱法同时测定动物源性食品中5种双酰胺类杀虫剂的残留[J].食品安全质量检测学报,2021,12(20):7977-7985 He X F,Huang X L,Xiao Q,et al.Simultaneous determination of 5 kinds of bisamide insecticides residues in animal-derived foods by QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry[J].Journal of Food Safety&Quality,2021,12(20):7977-7985(in Chinese)
盛祝波,汪杰,裴鸿艳,等.新型杀虫剂四唑虫酰胺[J].农药,2021,60(1):52-56 ,60Sheng Z B,Wang J,Pei H Y,et al.A new insecticide tetraniliprole[J].Agrochemicals,2021,60(1):52-56,60(inChinese)
谭海军.双酰胺类杀虫剂四唑虫酰胺的特点、合成与应用[J].世界农药,2021,43(9):32-42 ,56Tan H J.Characteristics,synthesis and application of a diamide insecticide:Tetraniliprole[J].World Pesticide,2021,43(9):32-42,56(in Chinese)
胡健,谷莉莉,仇学平,等.四唑虫酰胺对水稻稻纵卷叶螟的防治效果[J].现代农药,2022,21(5):70-72 Hu J,Gu L L,Qiu X P,et al.Control effect of tetraniliprole on Cnaphalocrocis medinalis[J].Modern Agrochemicals,2022,21(5):70-72(in Chinese)
陈朗,袁善奎,姜辉,等.双酰胺类杀虫剂环境风险问题浅析[J].农药科学与管理,2019,40(3):19-26 Chen L,Yuan S K,Jiang H,et al.A brief analysis on theenvironmental risk of diamide insecticides[J].PesticideScience and Administration,2019,40(3):19-26(in Chinese)
林涛,游泳,郑丽祯,等.三种双酰胺类杀虫剂制剂对环境非靶标生物的急性毒性[J].农药学学报,2015,17(6):757-762 Lin T,You Y,Zheng L Z,et al.Acute toxicity of three diamide insecticide preparations to environmental non-targetorganisms[J].Chinese Journal of Pesticide Science,2015,17(6):757-762(in Chinese)
Meng Z Y,Cui J J,Liu L,et al.Toxicity effects of chlorantraniliprole in zebrafish (Danio rerio) involving in liverfunction and metabolic phenotype[J].Pesticide Biochemistry and Physiology,2022,187:105194 Duan M M,Zhang J,Liu J,et al.Toxic effects of broflanilide exposure on development of zebrafish (Danio rerio) embryos and its potential cardiotoxicity mechanism[J].Environmental Pollution,2021,286:117481 国家技术监督局,国家环境保护局.水质物质对溞类(大型溞)急性毒性测定方法:GB/T 13266-1991[S].北京:国家技术监督局,国家环境保护局,1991 Li Y,Ma Y F,Yang L K,et al.Effects of azithromycin onfeeding behavior and nutrition accumulation of Daphniamagna under the different exposure pathways[J].Ecotoxicology and Environmental Safety,2020,197:110573 Wang P,Huang B M,Chen Z H,et al.Behavioural andchronic toxicity of fullerene to Daphnia magna:Mechanisms revealed by transcriptomic analysis[J].Environmental Pollution,2019,255:113181 陈彦吉,柳川江,宋怡彤,等.杀虫剂阿维菌素、伊维菌素对斜生栅藻和大型溞的毒性作用[J].生态毒理学报,2022,17(6):434-440 Chen Y J,Liu C J,Song Y T,et al.Toxic effects of avermectin and ivermectin on Scenedesmus obliquus andDaphnia magna[J].Asian Journal of Ecotoxicology,2022,17(6):434-440(in Chinese)
黄勃铭,吕小慧,王秋丽,等.富勒烯对大型溞的急性毒性和慢性毒性效应[J].农业环境科学学报,2017,36(4):620-624 Huang B M,LüX H,Wang Q L,et al.Toxicity assessments of fullerene to Daphnia magna:Acute toxicity andchronic toxicity[J].Journal of Agro-Environment Science,2017,36(4):620-624(in Chinese)
杨淞霖,尹晶,王会利,等.3种农药对大型溞的急性毒性比较[J].生态毒理学报,2017,12(2):238-242 Yang S L,Yin J,Wang H L,et al.The toxicity of threepesticides of mefenoxam,fludioxonil,azoxystrobin andtheir compounds to Daphnia magna Straus[J].AsianJournal of Ecotoxicology,2017,12(2):238-242(in Chinese)
Zubrod J P,Bundschuh M,Arts G,et al.Fungicides:An overlooked pesticide class?[J].Environmental Science&Technology,2019,53(7):3347-3365 Chagas T Q,da Silva Alvarez T G,Montalvão M F,et al.Behavioral toxicity of tannery effluent in zebrafish (Daniorerio) used as model system[J].The Science of the TotalEnvironment,2019,685:923-933 Bownik A,S'laska B,Dudka J.Cisplatin affects locomotor activity and physiological endpoints of Daphnia magna[J].Journal of Hazardous Materials,2020,384:121259 Zhang H Y,Ren X Y,Liu T,et al.The stereoselective toxicity of dinotefuran to Daphnia magna:A systematic assessment from reproduction,behavior,oxidative stress anddigestive function[J].Chemosphere,2023,327:138489 Cutler G C,Amichot M,Benelli G,et al.Hormesis andinsects:Effects and interactions in agroecosystems[J].The Science of the Total Environment,2022,825:153899 李玲,李俊杰,王俊英,等.手性农药水胺硫磷对浮游生物氧化应激的对映体选择性影响[J].生态毒理学报,2021,16(3):264-272 Li L,Li J J,Wang J Y,et al.Enantiomeric selectivity of chiral pesticide isocarbophos on oxidative stress in plankton[J].Asian Journal of Ecotoxicology,2021,16(3):264-272(in Chinese)
陈增龙,张紫溪,李莉,等.农药不同剂型间活性、毒性、行为和风险差异现状及其影响因素[J].农药,2023,62(8):547-554 ,569Chen Z L,Zhang Z X,Li L,et al.Differences in bioactivity,ecotoxicity,behavior and risk of different pesticideformulations and their influencing factors[J].Agrochemicals,2023,62(8):547-554,569(in Chinese)
Gobi N,Vaseeharan B,Rekha R,et al.Bioaccumulation,cytotoxicity and oxidative stress of the acute exposure selenium in Oreochromis mossambicus[J].Ecotoxicologyand Environmental Safety,2018,162:147-159 Saravanan A,Kumar P S,Vo D N,et al.A review on catalytic-enzyme degradation of toxic environmental pollutants:Microbial enzymes[J].Journal of Hazardous Materials,2021,419:126451 Luo T L,Chen J W,Li X H,et al.Effects of lomefloxacinon survival,growth and reproduction of Daphnia magnaunder simulated sunlight radiation[J].Ecotoxicology andEnvironmental Safety,2018,166:63-70 Lu Y,Zhang H,Wang H,et al.Humic acid mediated toxicity of faceted TiO2 nanocrystals to Daphnia magna[J].Journal of Hazardous Materials,2021,416:126112 Jin M K,Zhang Q,Zhao W L,et al.Fluoroquinolone antibiotics disturb the defense system,gut microbiome,andantibiotic resistance genes of Enchytraeus crypticus[J].Journal of Hazardous Materials,2022,424(Pt C):127509 Lv X H,Huang B M,Zhu X S,et al.Mechanisms underlying the acute toxicity of fullerene to Daphnia magna:Energy acquisition restriction and oxidative stress[J].Water Research,2017,123:696-703 Zhao Y C,Wang Z L,Li D T,et al.Two PBDEs exposureinducing feeding depression and disorder of digestive andantioxidative system of Daphnia magna[J].Ecotoxicologyand Environmental Safety,2019,176:279-287 毛曼菲,岳思青,赵美蓉.基于多组学技术的农药致毒机制研究进展[J].农药学学报,2019,21(S1):823-830 Mao M F,Yue S Q,Zhao M R.Advances in pesticidepoisoning mechanism based on multi-omics[J].ChineseJournal of Pesticide Science,2019,21(S1):823-830(inChinese)
乔治华,刘翔,姚向峰,等.溴氰虫酰胺暴露对赤子爱胜蚓的转录组学的影响[J].现代农药,2023,22(3):78-86 Qiao Z H,Liu X,Yao X F,et al.Effects of cyantraniliprole exposure on transcriptomics of earthworm (Eisenia fetida)[J].Modern Agrochemicals,2023,22(3):78-86(inChinese)
Brandts I,Teles M,Gonçalves A P,et al.Effects of nanoplastics on Mytilus galloprovincialis after individual andcombined exposure with carbamazepine[J].The Science of the Total Environment,2018,643:775-784 包鑫,陈小军,孟志远.农药对蚯蚓的毒理学效应研究进展[J].农药学学报,2023,25(3):551-571 Bao X,Chen X J,Meng Z Y.Research progress on toxicological effects of pesticides on earthworms[J].ChineseJournal of Pesticide Science,2023,25(3):551-571(inChinese)
Pan J M,Huang X H,Li Y X,et al.Zinc protects againstcadmium-induced toxicity by regulating oxidative stress,ions homeostasis and protein synthesis[J].Chemosphere,2017,188:265-273 FavéM J,Lamaze F C,Soave D,et al.Gene-by-environment interactions in urban populations modulate risk phenotypes[J].Nature Communications,2018,9(1):827 宋润芊,金玲敏,苏丽静,等.细胞色素P450酶代谢N-亚硝胺类化合物的反应机理研究[J].生态毒理学报,2023,18(3):68-78 Song R Q,Jin L M,Su L J,et al.Mechanisms of N-nitrosamines metabolized by cytochrome P450 enzymes[J].Asian Journal of Ecotoxicology,2023,18(3):68-78(inChinese)
Nair P C,McKinnon R A,Miners J O.Cytochrome P450structure-function:Insights from molecular dynamics simulations[J].Drug Metabolism Reviews,2016,48(3):434-452 -
点击查看大图
计量
- 文章访问数: 802
- HTML全文浏览数: 802
- PDF下载数: 178
- 施引文献: 0
下载:
