高级搜索

一种基于荧光素的高选择性硫化氢荧光探针

,  ,  , 

downloadPDF

上一篇

下一篇

刘春霞, 马兴, 魏国华, 杜宇国. 一种基于荧光素的高选择性硫化氢荧光探针[J]. 环境化学, 2014, 33(10): 1762-1767. doi: 10.7524/j.issn.0254-6108.2014.10.002
引用本文: 刘春霞, 马兴, 魏国华, 杜宇国. 一种基于荧光素的高选择性硫化氢荧光探针[J]. 环境化学, 2014, 33(10): 1762-1767. doi: 10.7524/j.issn.0254-6108.2014.10.002
shu
LIU Chunxia, MA Xing, WEI Guohua, DU Yuguo. A fluorescein-based probe for hydrogen sulfide detection with high selectivity[J]. Environmental Chemistry, 2014, 33(10): 1762-1767. doi: 10.7524/j.issn.0254-6108.2014.10.002
Citation: LIU Chunxia, MA Xing, WEI Guohua, DU Yuguo. A fluorescein-based probe for hydrogen sulfide detection with high selectivity[J]. Environmental Chemistry, 2014, 33(10): 1762-1767. doi: 10.7524/j.issn.0254-6108.2014.10.002
shu

一种基于荧光素的高选择性硫化氢荧光探针

  • 1.

    中国科学院生态环境科学研究中心, 环境化学与生态毒理学国家重点实验室, 北京, 100085;

  • 2.

    中国科学院大学化学与化学工程学院, 北京, 100049

  • 基金项目:

    国家自然科学基金(2011CB936001)资助.

A fluorescein-based probe for hydrogen sulfide detection with high selectivity

  • 1.

    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China;

  • 2.

    College of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, Beijing, 100049, China

  • Fund Project:

  • 摘要: 以荧光素作为荧光基团,基于硫化氢(H2S)的还原性,设计合成了一种off-on型H2S荧光探针.该探针本身在乙腈溶液中荧光十分微弱,与H2S反应之后,在520 nm处产生强荧光,同时溶液颜色由无色变成亮黄色,可实现对H2S的可视化检测.随着H2S浓度的逐渐增高,探针的荧光强度和紫外吸收强度也逐渐增强,在0—50 μmol·L-1的范围内,二者和H2S浓度之间都具有很好的线性关系,相关系数R2 分别为 0.9934、0.9921.探针对H2S具有很好的选择性,常见阴离子和还原性硫醇分子共存时,对检测不会产生干扰.该探针对H2S的检测限为10 μmol·L-1,具有较高的检测灵敏度.
    Abstract: We designed and synthesized a novel fluorescent probe 1 for hydrogen sulfide (H2S) detection based on the reductibility of H2S. The results indicated that a significant fluorescent enhancement at 520 nm and a bright yellow color appeared when H2S was added into the acetonitrile (CH3CN) solution of probe 1. The fluorescence intensity and absorption intensity strengthened gradually with the increasing concentrations of H2S. They have good linear relationships with H2S in the range of 0—50 μmol·L-1(R2= 0.9934, 0.9921). This new fluorescent probe shows excellent selectivity for H2S over other anions and reductive sulfurs, with a satisfactory detection limit at 10 μmol·L-1.
  • 加载中
  • [1] Szabo C. Hydrogen sulphide and its therapeutic potential[J].Nature Reviews Drug Discovery, 2007, 6(11): 917-935
    [2] Koshland D E. The molecule of the year[J].Science, 1992, 258(5090): 1861
    [3] Morita T, Perrella M A, Lee M E, et al. Smooth-muscle cell-derived carbon-monoxide is a regulator of vascular cGMP[J].Proceedings of the National Academy of Sciences, 1995, 92(5): 1475-1479
    [4] Gadalla M M, Snyder S H. Hydrogen sulfide as a gasotransmitter[J].Journal of Neurochemistry, 2010, 113(1): 14-26
    [5] Beauchamp R O, Bus J S, Popp J A, et al. A critical-review of the literature on hydrogen-sulfide toxicity[J].Critical Reviews in Toxicology, 1984, 13(1): 25-97
    [6] Reiffenstein R J, Hulbert W C, Roth S H. Toxicology of hydrogen-sulfide[J].Annual Review of Pharmacology and Toxicology, 1992, 32(1): 109-134
    [7] Moore P K, Bhatia M, Moochhala S. Hydrogen sulfide: From the smell of the past to the mediator of the future[J].Trends in Pharmacological Sciences, 2003, 24(12): 609-611
    [8] Boehning D, Snyder S H. Novel neural modulators[J].Annual Review of Neuroscience, 2003, 26(1): 105-131
    [9] Li L, Rose P, Moore P K. Hydrogen sulfide and cell signaling[J].Annual Review of Pharmacology and Toxicology, 2011, 51: 169-187
    [10] Balasubramanian S, Pugalenthi V. A comparative study of the determination of sulphide in tannery waste water by ion selective electrode (ISE) and iodimetry[J].Water Research, 2000, 34(17): 4201-4206
    [11] Pawlak Z, Pawlak A S. Modification of iodometric determination of total and reactive sulfide in environmental samples[J].Talanta, 1999, 48(2): 347-353
    [12] Lei W, Dasgupta P K. Determination of sulfide and mercaptans in caustic scrubbing liquor[J].Analytica Chimica Acta, 1989, 226(1): 165-170
    [13] Hughes M N, Centelles M N, Moore K P. Making and working with hydrogen sulfide: The chemistry and generation of hydrogen sulfide in vitro and its measurement in vivo: A review[J].Free Radical Biology and Medicine, 2009, 47(10): 1346-1353
    [14] Choi M G, Cha S, Lee H, et al. Sulfide-selective chemosignaling by a Cu2+ complex of dipicolylamine appended fluorescein[J].Chemical Communications, 2009, 38(47): 7390-7392
    [15] Pouly F, Touraud E, Buisson J F, et al. An alternative method for the measurement of mineral sulphide in wastewater[J].Talanta, 1999, 50(4): 737-742
    [16] Guenther E A, Johnson K S, Coale K H. Direct ultraviolet spectrophotometric determination of total sulfide and iodide in natural waters[J].Analytical Chemistry, 2001, 73(14): 3481-3487
    [17] Lawrence N S, Davis J, Jiang L, et al. The electrochemical analog of the methylene blue reaction: A novel amperometric approach to the detection of hydrogen sulfide[J].Electroanalysis, 2000, 12(18): 1453-1460
    [18] Doeller J E, Isbell T S, Benavides G, et al. Polarographic measurement of hydrogen sulfide production and consumption by mammalian tissues[J].Analytical Biochemistry, 2005, 341(1): 40-51
    [19] Radfordknoery J, Cutter G A. Determination of carbonyl sulfide and hydrogen-sulfide species in natural-waters using specialized collection procedures and gas-chromatography with flame photometric detection[J].Analytical Chemistry, 1993, 65(8): 976-982
    [20] Ubuka T, Abe T, Kajikawa R, et al. Determination of hydrogen sulfide and acid-labile sulfur in animal tissues by gas chromatography and ion chromatography[J].Journal of Chromatography B: Biomedical Sciences and Applications, 2001, 757(1): 31-37
    [21] Wu M Y, Li K, Hou J T, et al. A selective colorimetric and ratiometric fluorescent probe for hydrogen sulfide[J].Organic & Biomolecular Chemistry, 2012, 10(41): 8342-8347
    [22] Peng H, Cheng Y, Dai C, et al. A Fluorescent probe for fast and quantitative detection of hydrogen sulfide in blood[J].Angewandte Chemie International Edition, 2011, 50(41): 9672-9675
    [23] Wang X, Sun J, Zhang W, et al. A near-infrared ratiometric fluorescent probe for rapid and highly sensitive imaging of endogenous hydrogen sulfide in living cells[J].Chemical Science, 2013, 4(6): 2551-2556
    [24] Liu C, Pan J, Li S, et al. Capture and visualization of hydrogen sulfide by a fluorescent probe[J].Angewandte Chemie International Edition, 2011, 123(44): 10511-10513
    [25] Sasakura K, Hanaoka K, Shibuya N, et al. Development of a highly selective fluorescence probe for hydrogensulfide[J].Journal of the American Chemical Society, 2011, 133(45): 18003-18005
    [26] Wada T, Ohkubo A, Mochizuki A, et al. 2-(Azidomethyl) benzoyl as a new protecting group in nucleosides[J].Tetrahedron Letters, 2011, 42(6): 1069-1072
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  1979
  • HTML全文浏览数:  1979
  • PDF下载数:  671
  • 施引文献:  0
出版历程
  • 收稿日期:  2014-04-09
刘春霞, 马兴, 魏国华, 杜宇国. 一种基于荧光素的高选择性硫化氢荧光探针[J]. 环境化学, 2014, 33(10): 1762-1767. doi: 10.7524/j.issn.0254-6108.2014.10.002
引用本文: 刘春霞, 马兴, 魏国华, 杜宇国. 一种基于荧光素的高选择性硫化氢荧光探针[J]. 环境化学, 2014, 33(10): 1762-1767. doi: 10.7524/j.issn.0254-6108.2014.10.002
shu
LIU Chunxia, MA Xing, WEI Guohua, DU Yuguo. A fluorescein-based probe for hydrogen sulfide detection with high selectivity[J]. Environmental Chemistry, 2014, 33(10): 1762-1767. doi: 10.7524/j.issn.0254-6108.2014.10.002
Citation: LIU Chunxia, MA Xing, WEI Guohua, DU Yuguo. A fluorescein-based probe for hydrogen sulfide detection with high selectivity[J]. Environmental Chemistry, 2014, 33(10): 1762-1767. doi: 10.7524/j.issn.0254-6108.2014.10.002
shu

一种基于荧光素的高选择性硫化氢荧光探针

  • 1.  中国科学院生态环境科学研究中心, 环境化学与生态毒理学国家重点实验室, 北京, 100085;
  • 2.  中国科学院大学化学与化学工程学院, 北京, 100049
  • 收稿日期:  2014-04-09
基金项目:

国家自然科学基金(2011CB936001)资助.

摘要: 以荧光素作为荧光基团,基于硫化氢(H2S)的还原性,设计合成了一种off-on型H2S荧光探针.该探针本身在乙腈溶液中荧光十分微弱,与H2S反应之后,在520 nm处产生强荧光,同时溶液颜色由无色变成亮黄色,可实现对H2S的可视化检测.随着H2S浓度的逐渐增高,探针的荧光强度和紫外吸收强度也逐渐增强,在0—50 μmol·L-1的范围内,二者和H2S浓度之间都具有很好的线性关系,相关系数R2 分别为 0.9934、0.9921.探针对H2S具有很好的选择性,常见阴离子和还原性硫醇分子共存时,对检测不会产生干扰.该探针对H2S的检测限为10 μmol·L-1,具有较高的检测灵敏度.

English Abstract

    全文HTML

参考文献 (26)

返回顶部

目录

/

返回文章
返回

Copyright © 2019 中国科学院生态环境研究中心