PCR-DGGE技术用于处理苯乙烯废气的生物滴滤塔中微生物优势菌种解析

魏在山; 刘小红; 孙建良; 张再利; 马纯玉; 刘丽红

环境工程学报

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魏在山, 刘小红, 孙建良, 张再利, 马纯玉, 刘丽红. PCR-DGGE技术用于处理苯乙烯废气的生物滴滤塔中微生物优势菌种解析[J]. 环境工程学报, 2012, 6(2): 571-576.

引用本文:

魏在山, 刘小红, 孙建良, 张再利, 马纯玉, 刘丽红. PCR-DGGE技术用于处理苯乙烯废气的生物滴滤塔中微生物优势菌种解析[J]. 环境工程学报, 2012, 6(2): 571-576.

Wei Zaishan, Liu Xiaohong, Sun Jianliang, Zhang Zaili, Ma Chunyu, Liu Lihong. Microorganism analysis in biotrickling filter for treatment of waste gas containing styrene by PCR-DGGE[J]. Chinese Journal of Environmental Engineering, 2012, 6(2): 571-576.

Citation:

Wei Zaishan, Liu Xiaohong, Sun Jianliang, Zhang Zaili, Ma Chunyu, Liu Lihong. Microorganism analysis in biotrickling filter for treatment of waste gas containing styrene by PCR-DGGE[J]. Chinese Journal of Environmental Engineering, 2012, 6(2): 571-576.

PCR-DGGE技术用于处理苯乙烯废气的生物滴滤塔中微生物优势菌种解析

1.
中山大学环境科学与工程学院，广州 510275
基金项目:
珠海市科技计划项目产学研合作专项（PC20082029）

企业委托横向项目资助（7102261）

广州开发区科技计划项目重点科技攻关计划项目资助（2010Q-P161）
中图分类号: X701

Microorganism analysis in biotrickling filter for treatment of waste gas containing styrene by PCR-DGGE

1.
School of Environmental Science and Engineering， Sun Yat-sen University， Guangzhou 510275， China
Fund Project:

摘要: 采用生物滴滤塔能够有效去除含苯乙烯恶臭气体，塔内微生物中含有大量的球菌和杆状菌。采用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)技术研究处理苯乙烯恶臭气体的生物滴滤塔填料表面的微生物，结果表明，去除苯乙烯生物滴滤塔中有5种菌为降解苯乙烯的优势菌种；通过16S rDNA基因扩增测序同源性比对，结果显示嗜甲基杆菌属（methylophilus）丰度为50.5%，2种变形菌属（alpha proteobacterium、delta proteobacterium）相对丰度分别为16.9%和11.6%。
- 生物滴滤塔 /
- 恶臭废气 /
- 苯乙烯 /
- PCR-DGGE /
- 微生物
Abstract: Styrene removal could be achieved with high efficiency in the biotrickling filter packed with ceramsite. There are a large number of coccus and rod bacterial in the biotrickling filter. The microbial community structures in the biotrickling filter for styrene removal were assessed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Based on 16S rDNA sequence data， results show that the predominant bacterias for degradation of styrene are methylophilus， alpha proteobacterium， delta proteobacterium， two uncultured bacteriums. The dominant bacteria， methylophilus， takes up 50.5%； while alpha proteobacterium and delta proteobacterium are take up 16.9% and 11.6%， respectively.
- biotrickling filter /
- odor gas /
- styrene， PCR-DGGE /
- bacteria /

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魏在山, 刘小红, 孙建良, 张再利, 马纯玉, 刘丽红. PCR-DGGE技术用于处理苯乙烯废气的生物滴滤塔中微生物优势菌种解析[J]. 环境工程学报, 2012, 6(2): 571-576.

引用本文:

魏在山, 刘小红, 孙建良, 张再利, 马纯玉, 刘丽红. PCR-DGGE技术用于处理苯乙烯废气的生物滴滤塔中微生物优势菌种解析[J]. 环境工程学报, 2012, 6(2): 571-576.

Citation:

Wei Zaishan, Liu Xiaohong, Sun Jianliang, Zhang Zaili, Ma Chunyu, Liu Lihong. Microorganism analysis in biotrickling filter for treatment of waste gas containing styrene by PCR-DGGE[J]. Chinese Journal of Environmental Engineering, 2012, 6(2): 571-576.

PCR-DGGE技术用于处理苯乙烯废气的生物滴滤塔中微生物优势菌种解析

1. 中山大学环境科学与工程学院，广州 510275

收稿日期: 2011-02-27

基金项目:

珠海市科技计划项目产学研合作专项（PC20082029）

企业委托横向项目资助（7102261）

广州开发区科技计划项目重点科技攻关计划项目资助（2010Q-P161）

关键词:

摘要: 采用生物滴滤塔能够有效去除含苯乙烯恶臭气体，塔内微生物中含有大量的球菌和杆状菌。采用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)技术研究处理苯乙烯恶臭气体的生物滴滤塔填料表面的微生物，结果表明，去除苯乙烯生物滴滤塔中有5种菌为降解苯乙烯的优势菌种；通过16S rDNA基因扩增测序同源性比对，结果显示嗜甲基杆菌属（methylophilus）丰度为50.5%，2种变形菌属（alpha proteobacterium、delta proteobacterium）相对丰度分别为16.9%和11.6%。

English Abstract

Microorganism analysis in biotrickling filter for treatment of waste gas containing styrene by PCR-DGGE

1. School of Environmental Science and Engineering， Sun Yat-sen University， Guangzhou 510275， China

Received Date: 2011-02-27

Fund Project:

Keywords:

Abstract: Styrene removal could be achieved with high efficiency in the biotrickling filter packed with ceramsite. There are a large number of coccus and rod bacterial in the biotrickling filter. The microbial community structures in the biotrickling filter for styrene removal were assessed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Based on 16S rDNA sequence data， results show that the predominant bacterias for degradation of styrene are methylophilus， alpha proteobacterium， delta proteobacterium， two uncultured bacteriums. The dominant bacteria， methylophilus， takes up 50.5%； while alpha proteobacterium and delta proteobacterium are take up 16.9% and 11.6%， respectively.

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PCR-DGGE技术用于处理苯乙烯废气的生物滴滤塔中微生物优势菌种解析

Microorganism analysis in biotrickling filter for treatment of waste gas containing styrene by PCR-DGGE

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PCR-DGGE技术用于处理苯乙烯废气的生物滴滤塔中微生物优势菌种解析

English Abstract

Microorganism analysis in biotrickling filter for treatment of waste gas containing styrene by PCR-DGGE

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