镉对血管内皮细胞损伤及其致动脉硬化的毒理学机制

詹杰; 胡德奇; 柳承希; 魏树和; 张静生

1. 中国科学院沈阳应用生态研究所污染生态与环境工程重点实验室, 沈阳 110016;

2. 辽宁卫生职业技术学院营养与食品卫生研究室, 沈阳 110101;

3. 辽宁中医药大学, 沈阳 110101

作者简介: 詹杰(1972-),女,博士,研究方向:生态毒理,E-mail:yaoshanyuan@yahoo.com.cn

基金项目:

国家自然科学基金面上项目(31070455

40971184)

辽宁省自然科学基金项目(2004226010

2040278

201102224)

"十二五"国家科技支撑计划课题(2012BAC17B04)和"863"计划课题(2012AA06A202)。

中图分类号: X171.5

Damage of Cadmium to Vascular Endothelial Cells and Its Toxicity Mechanism in Atherosclerosis

1. Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;

2. Laboratory of Nutrition and Food Sanitation, Liaoning College of Health Vocational Technology, Shenyang 110101, China;

3. Liaoning University of Traditional Chinese Medicine, Shenyang 110101, China

Fund Project:

摘要: 采用人脐静脉内皮细胞体外培养和大鼠镉染毒实验,检测镉对人脐静脉内皮细胞和大鼠血清NO和ICAM-1分泌量,及大鼠主动脉病理改变的影响。结果表明,镉染毒可造成血管内皮细胞NO分泌量明显降低和ICAM-1分泌量明显升高,与镉染毒浓度剂量相关,体外细胞培养与活体动物实验的结果表现出一致性(P < 0.05)。镉暴露可明显促进血管内皮细胞凋亡,主动脉HE染色见动脉硬化改变,各层均见较多ICAM-1阳性表达,主要存在于粥样斑块区。因此,过量镉摄入通过影响大鼠血管内皮细胞功能和结构促进动脉粥样硬化的形成。

Abstract: Cultured in vitro and feed interventions experiments were conducted to explore the toxic effects of cadmium (Cd) on human umbilical vein endothelial cells (HUVECs) and rat artery. Results showed that NO level in Cd added group significantly decreased (P < 0.05), while the level of ICAM-1 in rat plasma significant increased (P < 0.05) compared with the control. Levels of NO and ICAM-1 in rat plasma were both in Cd dose-response manners. The results determined by cultured in vitro and living animal experiment were consistent (P < 0.05). The apoptosis of vascular endothelial cells and arteriosclerosis changes of aorta cross-section in HE staining promoted and caused by Cd was obviously observed. The immunohistochemically visualized ICAM-1 positively expressed on aortic intima and adventitia of the control group, which mainly distributed in the areas of atheromatous plaque. The results suggested that excessive intake of Cd affected the function and structure of endothelial cells, which probably promoted the formation of atherosclerosis.

Key words:

Cd /
atherosclerosis /
human umbilical vein endothelial cells /
rat

镉对血管内皮细胞损伤及其致动脉硬化的毒理学机制

作者简介: 詹杰(1972-),女,博士,研究方向:生态毒理,E-mail:yaoshanyuan@yahoo.com.cn
1. 中国科学院沈阳应用生态研究所污染生态与环境工程重点实验室, 沈阳 110016;

2. 辽宁卫生职业技术学院营养与食品卫生研究室, 沈阳 110101;

3. 辽宁中医药大学, 沈阳 110101

收稿日期: 2012-02-14

基金项目:

国家自然科学基金面上项目(31070455

40971184)

辽宁省自然科学基金项目(2004226010

2040278

201102224)

"十二五"国家科技支撑计划课题(2012BAC17B04)和"863"计划课题(2012AA06A202)。

关键词:

Damage of Cadmium to Vascular Endothelial Cells and Its Toxicity Mechanism in Atherosclerosis

1. Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;

2. Laboratory of Nutrition and Food Sanitation, Liaoning College of Health Vocational Technology, Shenyang 110101, China;

3. Liaoning University of Traditional Chinese Medicine, Shenyang 110101, China

Received Date: 2012-02-14

Fund Project:

Keywords:

Cd /
atherosclerosis /
human umbilical vein endothelial cells /
rat

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