苯并[a]芘和菲对缢蛏血细胞DNA损伤的研究
作者简介:
蒋玫(1973-),女,研究员,研究方向为海洋生态环境和渔业生态学,E-mail:jiangrose73@163.com
- 1. 中国水产科学研究院东海水产研究所;
- 2. 上海海洋大学
摘要: 为研究苯并[a]芘和菲对缢蛏的毒性效应,将缢蛏(Sinonovacula constricta)分别暴露于浓度为0.45 mg·L-1、0.15 mg·L-1、0.05 mg·L-1苯并[a]芘溶液和0.45 mg·L-1、0.15 mg·L-1、0.05 mg·L-1菲的溶液中,采用单细胞凝胶电泳实验(彗星实验)技术检测不同暴露时间缢蛏血淋巴细胞的DNA损伤程度,对照组为清洁海水。结果显示,高浓度(0.45 mg·L-1)苯并[a]芘溶液和(0.45 mg·L-1)菲溶液在短期(7 d)内即可导致缢蛏血细胞显著的DNA损伤,并且随苯并芘[a]和菲浓度的增大和暴露时间的延长,DNA损伤程度增加。21 d恢复实验后,各浓度组DNA损伤又均有不同程度的恢复,但中高浓度组(0.45 mg·L-1和0.15 mg·L-1)与对照组仍显著性差异。两种多环芳烃物质对缢蛏血细胞的DNA损伤作用均存在较显著时间-剂量-效应关系。其中,苯并芘[a]对缢蛏血细胞的DNA损伤作用要高于菲。
Effects of Benzo[a]pyrene and Phenanthrene on DNA Damage of Hemolymph Cell in Sinonovacula constricta
- 1. East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;
- 2. Shanghai Ocean University
- Received Date:
2015-02-08
Fund Project:
Abstract: The paper reported the DNA damage effect of Benzo[a]pyrene (Ba P) and phenanthrene (PHE) on the calm Sinonovacula constricta under different concentration conditions with the single cell microgel electrophoresis (SCGE) technique. The calm were treated with concentrations (Ba P:0.45, 0.15, 0.05 mg·L-1 and PHE:0.45, 0.15, 0.05 mg·L-1) in control group was 0.00 mg·L-1 for 15 days and were removed pollution for6 days. The result showed the tail DNA percentage (DNA%) and TL/D of the 0.45 mg·L-1 groups were significantly different from the control group (P<0.05) after 7 days exposure period. Furthermore, the damage intensity of treated groups increased gradually with respect to the increasing of Bap and PHE. Under the same concentration, the tail DNA% and TL/D increased gradually with the time of exposure. The DNA damage induced byBa P and PHE was very serious under the concentration of 0.45 mg·L-1. There was significant time-nd dose-dependent damage to the hemolymph cells. In addition, the toxic effect of Ba P was much more than PHE. The comet assay was proven to be a useful tool for detecting DNA damage induced by PAH in marine hemolymph cells. DNA damage in hemolymph cells of could indicate the effects of Ba P and PHE in marine environment as a good biomarker to be used in early warning and monitoring.
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