摘要:
探讨全氟辛烷磺酸钾(PFOS)和纳米氧化锌(Nano-ZnO)复合暴露对斑马鱼机体氧化损伤和细胞凋亡的影响。将斑马鱼胚胎暴露于PFOS(0、0.4、0.8和1.6 mg·L-1)、Nano-ZnO(0、12.5、25和50 mg·L-1)、PFOS+Nano-ZnO(0、0.4+12.5、0.8+25和1.6+50 mg·L-1)溶液中6天后,检测相关的酶活性变化(超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(Gpx)、脂质过氧化物(MDA)、半胱氨酸蛋白酶(Caspase-3和Caspase-9)和与细胞凋亡相关基因(Bax,p53和Bcl-2)表达情况。结果表明:PFOS和Nano-ZnO单独与复合暴露均可造成斑马鱼胚胎的氧化损伤和细胞凋亡,但复合暴露组的氧化损伤和细胞凋亡程度明显大于单独暴露组。在PFOS和Nano-ZnO单独和复合暴露组中,随着处理浓度的升高,SOD、Gpx、Caspase-3和Caspase-9酶的活性显著升高。而CAT酶活性随着处理浓度的升高抑制作用显著。PFOS与Nano-ZnO复合暴露组与单独暴露组相比,Bax和p53表达显著上调,而Bcl-2表达显著下调。因此,在实验浓度范围内,等毒性配比1:1条件下,推测NanoZnO可以增强PFOS对斑马鱼胚胎的氧化损伤和细胞凋亡毒性。
Abstract:
Oxidative stress and apoptosis to zebrafish (Danio rerio) embryos induced by perfluorooctanesul phonic acid potassium salt, (PFOS) and ZnO nanoparticles (Nano-ZnO) were investigated in this study. The embryos were exposed to PFOS (0、0.4、0.8 and 1.6 mg·L-1) single solutions, Nano-ZnO (0、12.5、25 and 50 mg·L-1)single solutions and PFOS plus Nano-ZnO (0、0.4 +12.5、0.8 +25 and 1.6 +50 mg·L-1) mixture solutions for 144 hour post-fertilisation (hpf). Activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (Gpx) and malondialdehyde (MDA) contents were measured in zebrafish after exposure for 144 hpf. At the same time, the levels of gene expression related to apoptosis (Bax, p53 and Bcl-2) were also measured. The results demonstrated that both PFOS and Nano-ZnO single-and co-exposure induced oxidative stress and apoptosis. In addition, PFOS plus Nano-ZnO exposure caused more serious oxidative stress and apoptosis than PFOS and Nano-ZnO single-exposure at above exposure concentrations. Several apoptosis pathways related genes such as Bax and p53 were significantly up-regulated in PFOS and Nano-ZnO co-exposure groups. Anti-apoptotic gene such as Bcl-2 was significantly down-regulated in PFOS and Nano-ZnO co-exposure groups. Under the toxicity ratio of 1:1, Nano-ZnO increased the toxicity of PFOS on oxidative stress and apoptosis in zebrafish embryos at the experimental concentrations.
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