摘要:
为探讨外源性甲醛是否会对小鼠肝脏和肾脏的热休克蛋白70(heat shock protein 70, Hsp70)产生影响，选雄性Balb/c小鼠为研究对象，采用动态吸入方式染毒2周，每周5 d，每天8 h，随机分为空白对照组、3 mg·m-3甲醛染毒组。染毒结束后，计算肝脏和肾脏的脏体比，检测各器官中活性氧(reactive oxygen species, ROS)、丙二醛(malondialdehyde, MDA)、还原型谷胱甘肽(glutathione, GSH)的含量，同时用实时荧光定量PCR(real-time PCR, RT-PCR)法检测Hsp70的基因表达，并通过H & E染色观察形态学变化。结果表明：与对照组相比，2种器官的脏体比均显著下降(P<0.05或P<0.01)；ROS、MDA含量上升、GSH含量下降，出现显著性差异(P<0.05或P<0.01)；Hsp70基因表达水平下降且差异显著(P<0.05或P<0.01)；H & E切片显示，甲醛暴露使肝脏的中央静脉窦的破损程度增加、细胞核变大，肾脏中肾小中肾小球基底膜变窄、体积缩小甚至分解。上述结果表明，甲醛暴露会对小鼠的的肝脏和肾脏造成不同程度的氧化损伤，小鼠可通过上调Hsp70的基因表达以增强机体的抗氧化能力。
Abstract:
In order to explore the role of heat shock protein 70 (Hsp70) in the toxic effect of exogenous formaldehyde (FA), male Balb/c mice were exposed to FA (3 mg·m-3) or clean room air by nose-only inhalation for 8 h·d-1, 5 days per week for two weeks. At the next day of the last-day exposure, the live and kidney tissues were collected for the terminal detection. Compared with the control group, the organ coefficients of liver or kidney tissues in the FA group significantly decreased (P<0.05 or P<0.01). The content of reactive oxygen species (ROS) and malondialdehyde (MDA) both significantly increased (P<0.05 or P<0.01), while the level of glutathione (GSH) decreased significantly (P<0.05 or P<0.01). Meanwhile, the gene expression level of Hsp70 was detected by real-time PCR (RT-PCR), and reduced significantly (P<0.05 or P<0.01). Pathological data of the H & E staining slices showed that FA exposure aggravated the damage on the central venous sinus, and the nucleuses were enlarged in the liver. What’s more, the glomerular basement membrane of kidney became narrower, and its volume was reduced or even decomposed. The results indicated that the oxidative stress induced by FA in kidney and liver tissues could be reversed by up-regulating Hsp70 gene expression.
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