四唑虫酰胺对大型溞的急性毒性和毒理
Acute Toxicity and Toxicology of Tetraniliprole on Daphnia magna
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摘要: 四唑虫酰胺是新一代双酰胺类杀虫剂，具有广阔应用前景，阐明其环境安全性具有重要意义。在本研究中，评估了四唑虫酰胺对水生生物大型溞的生态毒性效应。研究结果表明，四唑虫酰胺对大型溞具有较高的急性毒性风险，其48 h-LC50值为（0.096±0.010） mg·L-1。在0.005 mg·L-1和0.025 mg·L-1，四唑虫酰胺对大型溞的摄食行为和运动行为具有不同程度的抑制作用。此外，四唑虫酰胺导致大型溞体内超氧化物歧化酶（superoxide dismutase，SOD）活性显著升高，而乙酰胆碱酯酶（acetylcholinesterase，AChE）、谷胱甘肽硫转移酶（glutathione S-transferases，GST）和胰蛋白酶（trypsin）活性显著降低。转录组测序结果显示，随着暴露浓度的增加，四唑虫酰胺在大型溞体内诱导的差异表达基因数量逐渐增多。差异表达基因KEGG富集分析结果显示，不同剂量的四唑虫酰胺均显著影响了大型溞体内与代谢和蛋白质合成功能相关信号通路。关键差异基因筛选和验证结果表明，与抗氧化功能相关基因表达量在各四唑虫酰胺处理组均显著上调，而与解毒、消化和核糖体功能相关基因表达量在各四唑虫酰胺处理组均显著下调。本研究阐明了四唑虫酰胺对大型溞的急性风险，并揭示了亚致死浓度下，四唑虫酰胺对大型溞生理功能的影响，相关结果可为四唑虫酰胺在水环境中的环境安全性提供理论依据。Abstract: Tetraniliprole is a new generation of diamide insecticide, which has broad application prospects, and it is important to clarify its environmental safety. In this study, the toxic effects of tetraniliprole on the aquatic organism Daphnia magna were evaluated. The present results show that tetraniliprole has a high risk of acute toxicity to Daphnia magna, evidenced by a LC50 value of (0.096±0.010) mg·L-1 at 48 h. At concentrations of 0.005 mg·L-1 and 0.025 mg·L-1, tetraniliprole inhibited the feeding behavior and motor behavior of Daphnia magna to varying degrees. Additionally, the activities of superoxide dismutase (SOD) in Daphnia magna were significantly enhanced by tetraniliprole, while the activities of acetylcholinesterase (AChE), glutathione S-transferase (GST) and trypsin were significantly inhibited. Transcriptome sequencing results showed that the number of differentially expressed genes (DEGs) induced by tetraniliprole increased gradually with rising exposure concentrations. The KEGG enrichment analysis showed that the metabolism and protein synthesis related signaling pathways were significantly affected by different doses of tetraniliprole. Screening and verification of key DEGs showed that the expression levels of antioxidant-related genes were significantly up-regulated in each tetraniliprole exposure treatment, while the expression levels of genes associated with detoxification, ribosome function and digestion were significantly down-regulated in each tetraniliprole exposure treatment. This study not only elucidates the acute risk of tetraniliprole to Daphnia magna but also unveils the physiological effects of tetraniliprole on the function of Daphnia magna at sublethal concentrations. The obtained insights can provide theoretical basis for the environmental safety of tetraniliprole in aquatic environment.
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Key words:
- pesticide /
- diamide insecticides /
- Daphnia magna /
- acute toxicity /
- transcriptome sequencing /
- gene expression /
- risk assessment
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