摘要:
为了探讨双酚A(bisphenol A,BPA)对海洋微藻的生态毒性效应,实验选择了以青岛大扁藻(Platymonas helgolanidica)作为受试物种,设置6个实验浓度(即0、2、4、6、8、10 mg·L-1)对微藻进行了96 h暴露处理,测定了不同浓度暴露下对青岛大扁藻的生长以及抗氧化系统酶活性等指标。研究结果表明,BPA对青岛大扁藻的96h-EC50为9.32 mg·L-1,属高毒类污染物。青岛大扁藻经过BPA暴露处理后,细胞密度下降,细胞色素含量降低,并且呈现明显的剂量-效应关系;细胞抗氧化系统中超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)、单脱氢抗坏血酸还原酶(MDHAR)、脱氢抗坏血酸还原酶(DHAR)、谷胱甘肽还原酶(GR)活性均受到干扰。
Abstract:
The effects of bisphenol A (BPA) on the growth of Platymonas helgolanidica were studied, so as to explore the ecological toxicity effects of BPA on marine microalgae. The exposure concentrations of BPA were 0, 2, 4, 6, 8, and 10 mg·L-1. The parameters such as cell densities, antioxidant enzyme systems, as well as 96 h EC50 of P. helgolanidica under different BPA concentrations were measured during the experimental period. The results showed that 96h-EC50 of BPA for P. helgolanidica was 9.32 mg·L-1, indicating that BPA was a high toxic pollutant to P. helgolanidica. When P. helgolanidica was exposed to BPA at the above concentrations, the growth of P. helgolanidica was inhibited:both the cell density and cell pigment content was decreased, which was demonstrated by dose effect-relationships. Besides, the activities of superoxide dismutase (SOD), ascorbic acid peroxidase (APX), dehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), and glutathione reductase (GR) in cells were disturbed in the study.
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