水环境中农药类污染物的内分泌干扰效应研究
Endocrine Disrupting Effects of Pesticides in Water Environment
-
摘要: 水环境中农药类内分泌干扰物与人类健康密切相关。以水源水中筛查鉴定出的15种农药类污染物为对象，采用化学激活荧光素酶基因表达法(CALUX)，进行了雌激素受体(ER)、雄激素受体(AR)、肾上腺皮质激素受体(GR)激动和拮抗效应筛查。研究结果显示所有测试物均未显示激素受体激动效应，环莠隆具有ER拮抗效应，多效唑、戊唑醇和三环唑表现出AR拮抗效应，阿特拉津、扑草净、三环唑和甲霜灵表现出GR拮抗作用。研究结果为水环境中农药类污染物的风险评估提供支撑。
-
关键词:
- 农药 /
- 化学诱导荧光素酶表达基因法 /
- 内分泌干扰物 /
- 综合激素效应
Abstract: Pesticide endocrine disruptors in water environment are closely related to human health. In this study, the chemical activated luciferase gene expression (CALUX) was used to screen the promoting and antagonistic effects of 15 pesticide pollutants identified in source water, including estrogen receptor (ER), androgen receptor (AR) and glucocorticoid receptor (GR). None of the tested substances showed hormonal receptor promoting effect. Cycluron was shown as estrogen receptor antagonistic effect. Paclobutrazol, tebuconazole and tricyclazole were shown as androgen receptor antagonistic effect. Atrazine, prometryn, tricyclazole and metalaxyl were shown as glucocorticoid receptor antagonistic activity. The research results provide support for the risk assessment of pesticide pollutants in water environment. -
-
Sun J, Luo Q, Wang D H, et al. Occurrences of pharmaceuticals in drinking water sources of major river watersheds, China [J]. Ecotoxicology and Environmental Safety, 2015, 117: 132-140 de Jesus Gaffney V, Almeida C M, Rodrigues A, et al. Occurrence of pharmaceuticals in a water supply system and related human health risk assessment [J]. Water Research, 2015, 72: 199-208 Blair B D, Crago J P, Hedman C J, et al. Pharmaceuticals and personal care products found in the Great Lakes above concentrations of environmental concern [J]. Chemosphere, 2013, 93(9): 2116-2123 Simonyan A, Gabrielyan B, Minasyan S, et al. Genotoxicity of water contaminants from the basin of Lake Sevan, Armenia evaluated by the comet assay in gibel carp (Carassius auratus gibelio) and Tradescantia bioassays [J]. Bulletin of Environmental Contamination and Toxicology, 2016, 96(3): 309-313 Malakahmad A, Manan T S B A, Sivapalan S, et al. Genotoxicity assessment of raw and treated water samples using Allium cepa assay: Evidence from Perak River, Malaysia [J]. Environmental Science and Pollution Research International, 2018, 25(6): 5421-5436 Dhaini H R, Nassif R M. Exposure assessment of endocrine disruptors in bottled drinking water of Lebanon [J]. Environmental Monitoring and Assessment, 2014, 186(9): 5655-5662 韩志华, 卜元卿, 单正军, 等. 内分泌干扰类农药生物检测技术的研究进展[J]. 生态毒理学报, 2011, 6(5): 449-458 Han Z H, Bu Y Q, Shan Z J, et al. Review on bioassay technology of endocrine disrupting pesticides [J]. Asian Journal of Ecotoxicology, 2011, 6(5): 449-458 (in Chinese)
方琪, 马彦博, 张思远, 等. 农药内分泌干扰效应研究进展[J]. 生态毒理学报, 2017, 12(1): 98-110 Fang Q, Ma Y B, Zhang S Y, et al. Research progress in endocrine disrupting effects of pesticides [J]. Asian Journal of Ecotoxicology, 2017, 12(1): 98-110 (in Chinese)
Streck G. Chemical and biological analysis of estrogenic, progestagenic and androgenic steroids in the environment [J]. Trends in Analytical Chemistry, 2009, 28(6): 635-652 张诺, 孙韶华, 王明泉, 等. 荧光素酶表达基因法(CALUX)用于二噁英检测的研究进展[J]. 生态毒理学报, 2014, 9(3): 391-397 Zhang N, Sun S H, Wang M Q, et al. Research progress of dioxins detection using chemical-luciferase gene expression (CALUX) [J]. Asian Journal of Ecotoxicology, 2014, 9(3): 391-397 (in Chinese)
Seeger B, Klawonn F, Bekale B N, et al. The ability of the YAS and AR CALUX assays to detect the additive effects of anti-androgenic fungicide mixtures [J]. Toxicology Letters, 2016, 241: 193-199 逯南南, 许燕, 赵清华, 等. 三氯乙烯和四氯乙烯的细胞毒性及氧化应激机制研究[J]. 生态毒理学报, 2018, 13(6): 326-332 Lu N N, Xu Y, Zhao Q H, et al. Cytotoxicity of TCE and PCE to CHO cells and the oxidative stress mechanism [J]. Asian Journal of Ecotoxicology, 2018, 13(6): 326-332(in Chinese)
Wielogórska E, Elliott C T, Danaher M, et al. Endocrine disruptor activity of multiple environmental food chain contaminants [J]. Toxicology in Vitro: An International Journal Published in Association with BIBRA, 2015, 29(1): 211-220 van der Linden S C, Heringa M B, Man H Y, et al. Detection of multiple hormonal activities in wastewater effluents and surface water, using a panel of steroid receptor CALUX bioassays [J]. Environmental Science & Technology, 2008, 42(15): 5814-5820 Orton F, Rosivatz E, Scholze M, et al. Widely used pesticides with previously unknown endocrine activity revealed as in vitro antiandrogens [J]. Environmental Health Perspectives, 2011, 119(6): 794-800 郭佳, 杨雪, 张灿, 等. 呋喃丹和氯氰菊酯的类雌激素活性研究[J]. 农药, 2012, 51(2): 105-107 , 150 Guo J, Yang X, Zhang C, et al. Exploring the estrogenicity of carbofuran and cypermethrin [J]. Agrochemicals, 2012, 51(2): 105-107, 150 (in Chinese)
Mnif W, Hassine A I, Bouaziz A, et al. Effect of endocrine disruptor pesticides: A review [J]. International Journal of Environmental Research and Public Health, 2011, 8(6): 2265-2303 陈秀云, 祝娉婷, 孙鹏玲, 等. 基因重组酵母测评系统在筛选环境样品雌激素活性中的应用研究[J]. 环境与健康杂志, 2009, 26(11): 965-969 Chen X Y, Zhu P T, Sun P L, et al. Application of gene recombinant yeast cell in screening to environmental estrogen [J]. Journal of Environment and Health, 2009, 26(11): 965-969 (in Chinese)
Kojima H, Sata F, Takeuchi S, et al. Comparative study of human and mouse pregnane X receptor agonistic activity in 200 pesticides using in vitro reporter gene assays [J]. Toxicology, 2011, 280(3): 77-87 Song Q, Zhang Y, Yan L, et al. Risk assessment of the endocrine-disrupting effects of nine chiral pesticides [J]. Journal of Hazardous Materials, 2017, 338: 57-65 Cooper R L, Stoker T E, Tyrey L, et al. Atrazine disrupts the hypothalamic control of pituitary-ovarian function [J]. Toxicological Sciences: An Official Journal of the Society of Toxicology, 2000, 53(2): 297-307 Sanderson J T, Seinen W, Giesy J P, et al. 2-Chloro-s-triazine herbicides induce aromatase (CYP19) activity in H295R human adrenocortical carcinoma cells: A novel mechanism for estrogenicity? [J]. Toxicological Sciences: An Official Journal of the Society of Toxicology, 2000, 54(1): 121-127 Zhang J Y, Zhang J, Liu R, et al. Endocrine-disrupting effects of pesticides through interference with human glucocorticoid receptor [J]. Environmental Science & Technology, 2016, 50(1): 435-443 Trösken E R, Scholz K, Lutz R W, et al. Comparative assessment of the inhibition of recombinant human CYP19 (aromatase) by azoles used in agriculture and as drugs for humans [J]. Endocrine Research, 2004, 30(3): 387-394 Hurst M R, Sheahan D A. The potential for oestrogenic effects of pesticides in headwater streams in the UK [J]. Science of the Total Environment, 2003, 301(1-3): 87-96 Rieke S, Koehn S, Hirsch-Ernst K, et al. Combination effects of (tri)azole fungicides on hormone production and xenobiotic metabolism in a human placental cell line [J]. International Journal of Environmental Research and Public Health, 2014, 11(9): 9660-9679 Morinaga H, Yanase T, Nomura M, et al. A benzimidazole fungicide, benomyl, and its metabolite, carbendazim, induce aromatase activity in a human ovarian granulose-like tumor cell line (KGN) [J]. Endocrinology, 2004, 145(4): 1860-1869 Kucka M, Pogrmic-Majkic K, Fa S, et al. Atrazine acts as an endocrine disrupter by inhibiting cAMP-specific phosphodiesterase-4 [J]. Toxicology and Applied Pharmacology, 2012, 265(1): 19-26 -
点击查看大图
计量
- 文章访问数: 1742
- HTML全文浏览数: 1742
- PDF下载数: 73
- 施引文献: 0
下载:
