摘要:
纳米Fe3O4作为一种功能材料,在生物医药、生物靶向材料、微波吸收材料和高梯度磁分离器等方面应用前景广阔,其潜在的生物毒性也备受关注。为研究纳米Fe3O4对生物体可能造成的氧化损伤,以昆明小鼠为受试体,设置5、10、20和40 mg· kg-1 4个染毒组,腹腔注射染毒7 d后,测定小鼠肺组织中活性氧(reactive oxygen species,ROS)、还原型谷胱甘肽(glutathione,GSH)和丙二醛(malondialdehyde,MDA)的含量。结果显示,随着纳米Fe3O4染毒剂量的升高,肺组织ROS和MDA含量逐渐上升,GSH含量逐渐降低,各指标均呈一定的剂量-效应关系。剂量≥ 10 mg·kg-1,肺组织ROS含量与对照组相比有显著差异(p < 0.05);剂量≥ 20 mg·kg-1,肺组织MDA含量与对照组相比有显著差异(p < 0.05);剂量≥ 40 mg·kg-1,肺组织GSH含量与对照组相比有显著差异(p < 0.05)。研究表明,较高剂量(≥ 20 mg·kg-1)的纳米Fe3O4颗粒材料会引起小鼠肺细胞的氧化损伤。
关键词:
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纳米Fe3O4
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小鼠
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肺
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氧化损伤
Abstract:
Nano-Fe3O4, as a functional material, has a wide range of applications in the fields of biomedicine, biotargeted materials, microwave absorbing materials, high gradient magnetic separators and etc., so study on the biotoxicity of nano-Fe3O4 has attracted increasing attention. To investigate the potential oxidative damages of Fe3O4 nanoparticles to organisms, Kunming mice were intraperitoneally administrated with Fe3O4 nanoparticles for seven days at the doses of 5, 10, 20 and 40 mg·kg-1, respectively. The contents of ROS (reactive oxygen species), GSH (glutathione) and MDA (malondialdehyde) in the lung homogenate were measured to indicate the oxidative damage. Results showed that the contents of ROS and MDA increased gradually while GSH content decreased with the increase in exposure dose, and all the studied biomarkers changed in dose-response manners. When exposure dose exceeded 10 mg·kg-1, ROS content was significantly higher than that of the control group (p < 0.05); when exposure dose was over 20 mg·kg-1, MDA content was significantly higher than that of the control group (p < 0.05); when exposure dose was higher than 40 mg·kg-1, GSH content turned out to have significant difference compared with the control group (p < 0.05). It is demonstrated that Fe3O4 nanoparticles can increase the oxidative stress and cause oxidative damage in mouse lung at relatively high dose (≥ 20 mg·kg-1).
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