背角无齿蚌超氧化物歧化酶基因的克隆及多溴联苯醚-47和多溴联苯醚-209对其表达的影响
Effects of PBDE-47 and PBDE-209 on the AwSOD Expression in Freshwater Bivalve Anodonta woodiana
-
摘要: 超氧化物歧化酶（SOD）是机体防御和氧化应激损伤的关键酶之一。为了探讨多溴联苯醚PBDE-47和PBDE-209对背角无齿蚌的胁迫效应，本研究克隆出AwSOD全基因序列，分析PBDE-47和PBDE-209对AwSOD表达的影响。背角无齿蚌AwSOD cDNA全长由949个核苷酸组成，开放阅读框包含465 bp核苷酸，编码155个氨基酸。AwSOD包含Cu/ZnSOD家族GKHGFHVHEFGDNT和GNAGARSACGVI这2个特征性标签序列。AwSOD氨基酸序列与Cu/ZnSOD具有较高的同源性。AwSOD与淡水贝类亲缘关系最近，其次海洋双壳类，最后是脊椎动物、腹足类和甲壳类。AwSOD在肝胰脏中表达水平较高，在鳃和斧足为中等水平，在外套膜、闭壳肌和心脏中表达水平较低。与对照组相比，PBDE-47和PBDE-209处理对肝胰腺中AwSOD mRNA水平具有显著的诱导作用。PBDE-47处理对鳃中AwSOD mRNA水平在早期具有显著诱导作用，高剂量组在后期呈现下调效应；PBDE-209处理后鳃中AwSOD mRNA水平显著增加。结果表明，PBDE-47和PBDE-209对背角无齿蚌AwSOD表达水平诱导作用与增强机体抗氧化能力和胁迫效应有关。Abstract: The superoxide dismutases (SODs) play a key role against oxidative stress. In order to explore the stress of polybrominated diphenyl ethers (PBDEs) on the bivalve Anodonta woodiana, one complete Cu/ZnSOD sequence was isolated and named AwSOD in the current study. The full-length cDNA of AwSOD had an open reading frame (ORF) of 465 bp encoding 155 amino acids. The deduced amino acid sequence of AwSOD has high identity with the Cu/ZnSODs. The most relationship of evolution of AwSOD was that of freshwater bivalve, next was marine bivalve, last was vertebrate, gastropods and crustacean. In selected tissues, the highest expression of AwSOD mRNA was observed in the hepatopancreas, intermediate ones in the foot, adductor muscle, and gill, lower ones in heart and mantle. Compared with that of control group, AwSOD mRNA level of hepatopancreas was significantly induced by PBDE-47 and PBDE-209. AwSOD expression of gill was significantly up-regulated at early stage in the PBDE-47 treated groups, and down-regulated at later stage in the high-dose groups in contrasted with that of control group. AwSOD mRNA level of gill was significantly increased in the PBDE-209 treated goups. These results indicate that up-regulations of AwSOD expression of hepatopancreas and gill contributed to elimination of stress derived from PBDE-47 and PBDE-209 challenge in the freshwater bivalve A. woodiana.
-
-
Fromme H, Becher G, Hilger B, et al. Brominated flame retardants-Exposure and risk assessment for the general population[J]. International Journal of Hygiene and Environmental Health, 2016, 219(1):1-23 Roberts S C, Noyes P D, Gallagher E P, et al. Species-specific differences and structure-activity relationships in the debromination of PBDE congeners in three fish species[J]. Environmental Science & Technology, 2011, 45(5):1999-2005 Zhang R Q, Guo J Y, Wu F C, et al. Toxicity reference values for polybrominated diphenyl ethers:Risk assessment for predatory birds and mammals from two Chinese lakes[J]. Reviews of Environmental Contamination and Toxicology, 2014, 229:111-137 Li Z H, Panton S, Marshall L, et al. Spatial analysis of polybrominated diphenylethers (PBDEs) and polybrominated biphenyls (PBBs) in fish collected from UK and proximate marine waters[J]. Chemosphere, 2018, 195:727-734 Tan K, Zhang B, Ma H Y, et al. Oxidative stress responses of golden and brown noble scallops Chlamys nobilis to acute cold stress[J]. Fish & Shellfish Immunology, 2019, 95:349-356 Umasuthan N, Bathige S D, Revathy K S, et al. A manganese superoxide dismutase (MnSOD) from Ruditapes philippinarum:Comparative structural- and expressional-analysis with copper/zinc superoxide dismutase (Cu/ZnSOD) and biochemical analysis of its antioxidant activities[J]. Fish & Shellfish Immunology, 2012, 33(4):753-765 Che M X, Wang R, Li X X, et al. Expanding roles of superoxide dismutases in cell regulation and cancer[J]. Drug Discovery Today, 2016, 21(1):143-149 Yang W Y, Liu W X, Wen C G, et al. A superoxide dismutase (MnSOD) with identification and functional characterization from the freshwater mussel Cristaria plicata[J]. Fish & Shellfish Immunology, 2019, 91:180-187 Zhou Z, Liu Z Q, Wang L G, et al. Oxidative stress, apoptosis activation and symbiosis disruption in giant clam Tridacna crocea under high temperature[J]. Fish & Shellfish Immunology, 2019, 84:451-457 Kim U J, Jo H, Lee I S, et al. Investigation of bioaccumulation and biotransformation of polybrominated diphenyl ethers, hydroxylated and methoxylated derivatives in varying trophic level freshwater fishes[J]. Chemosphere, 2015, 137:108-114 Soo P, Todd P A. The behaviour of giant clams (Bivalvia:Cardiidae)[J]. Marine Biology, 2014, 161(12):2699-2717 Xia X C, Yu R X, Li M B, et al. Molecular cloning and characterization of two genes encoding peroxiredoxins from freshwater bivalve Anodonta woodiana:Antioxidative effect and immune defense[J]. Fish & Shellfish Immunology, 2018, 82:476-491 Jing W X, Lang L, Lin Z G, et al. Cadmium bioaccumulation and elimination in tissues of the freshwater mussel Anodonta woodiana[J]. Chemosphere, 2019, 219:321-327 Davies R, Zou E M. Polybrominated diphenyl ethers disrupt molting in neonatal Daphnia magna[J]. Ecotoxicology, 2012, 21(5):1371-1380 Wang C, Huan P, Yue X, et al. Molecular characterization of a glutathione peroxidase gene and its expression in the selected Vibrio-resistant population of the clam Meretrix meretrix[J]. Fish & Shellfish Immunology, 2011, 30(6):1294-1302 Li C H, Ni D J, Song L S, et al. Molecular cloning and characterization of a catalase gene from Zhikong scallop Chlamys farreri[J]. Fish & Shellfish Immunology, 2008, 24(1):26-34 Bao Y B, Li L, Wu Q, et al. Cloning, characterization, and expression analysis of extracellular copper/zinc superoxide dismutase gene from bay scallop Argopecten irradians[J]. Fish & Shellfish Immunology, 2009, 27(1):17-25 Xu H H, Ma H, Hu B Q, et al. Molecular cloning, identification and functional characterization of a novel intracellular Cu-Zn superoxide dismutase from the freshwater mussel Cristaria plicata[J]. Fish & Shellfish Immunology, 2010, 29(4):615-622 Atli G, Canli E G, Eroglu A, et al. Characterization of antioxidant system parameters in four freshwater fish species[J]. Ecotoxicology and Environmental Safety, 2016, 126:30-37 Park H, Ahn I Y, Lee J K, et al. Molecular cloning, characterization, and the response of manganese superoxide dismutase from the Antarctic bivalve Laternula elliptica to PCB exposure[J]. Fish & Shellfish Immunology, 2009, 27(3):522-528 Lyu K, Zhu X X, Chen R, et al. Molecular cloning of manganese superoxide dismutase gene in the cladoceran Daphnia magna:Effects of microcystin, nitrite, and cadmium on gene expression profiles[J]. Aquatic Toxicology, 2014, 148:55-64 Gómez-Anduro G A, Ascencio-Valle F, Peregrino-Uriarte A B, et al. Cytosolic manganese superoxide dismutase genes from the white shrimp Litopenaeus vannamei are differentially expressed in response to lipopolysaccharides, white spot virus and during ontogeny[J]. Comparative Biochemistry and Physiology Part B, Biochemistry & Molecular Biology, 2012, 162(4):120-125 Woo S, Yum S, Park H S, et al. Effects of heavy metals on antioxidants and stress-responsive gene expression in Javanese medaka (Oryzias javanicus)[J]. Comparative Biochemistry and Physiology Toxicology & Pharmacology, 2009, 149(3):289-299 Hung M N, Shiomi R, Nozaki R, et al. Identification of novel copper/zinc superoxide dismutase (Cu/ZnSOD) genes in kuruma shrimp Marsupenaeus japonicas[J]. Fish & Shellfish Immunology, 2014, 40(2):472-477 Cheng W, Tung Y H, Liu C H, et al. Molecular cloning and characterisation of copper/zinc superoxide dismutase (Cu, Zn-SOD) from the giant freshwater prawn Macrobrachium rosenbergii[J]. Fish & Shellfish Immunology, 2006, 21(1):102-112 Bautista-Covarrubias J C, Aguilar-Juárez M, Voltolina D, et al. Immunological response of white shrimp (Litopenaeus vannamei) to sublethal concentrations of malathion and endosulfan, and their mixture[J]. Ecotoxicology and Environmental Safety, 2020, 188:109893 郑双艳, 孟超, 刘清, 等. 重金属对褶纹冠蚌Cu/ZnSOD基因mRNA表达和酶活性的影响[J]. 南昌大学学报:理科版, 2018, 42(1):67-71 Zheng S Y, Meng C, Liu Q, et al. Effects of heavy metal on mRNA expression and enzymatic activity of Cu/Zn superoxide dismutase in Cristaria plicata[J]. Journal of Nanchang University:Natural Science, 2018, 42(1):67-71(in Chinese)
Wollenberger L, Dinan L, Breitholtz M. Brominated flame retardants:Activities in a crustacean development test and in an ecdysteroid screening assay[J]. Environmental Toxicology and Chemistry, 2005, 24(2):400-407 de Andrade K Q, Moura F A, dos Santos J M, et al. Oxidative stress and inflammation in hepatic diseases:Therapeutic possibilities of N-acetylcysteine[J]. International Journal of Molecular Sciences, 2015, 16(12):30269-30308 Hallmann A, Konieczna L, Swiezak J, et al. Aromatisation of steroids in the bivalve Mytilus trossulus[J]. PeerJ, 2019, 7:e6953 Hou L, Jiang J Y, Gan Z W, et al. Spatial distribution of organophosphorus and brominated flame retardants in surface water, sediment, groundwater, and wild fish in Chengdu, China[J]. Archives of Environmental Contamination and Toxicology, 2019, 77(2):279-290 Fu L F, Pei J, Zhang Y Y, et al. Polybrominated diphenyl ethers and alternative halogenated flame retardants in mollusks from the Chinese Bohai Sea:Levels and interspecific differences[J]. Marine Pollution Bulletin, 2019, 142:551-558 -
点击查看大图
计量
- 文章访问数: 1102
- HTML全文浏览数: 1102
- PDF下载数: 24
- 施引文献: 0
下载:
