全氟辛烷磺酸(PFOS)对斑马鱼血浆和组织匀浆中卵黄蛋白原含量的影响
作者简介:
程艳(1977-),女,博士,副研究员,研究方向为生态毒理学,E-mail:yanzi77pku@yahoo.com.cn
- 1. 中国检验检疫科学研究院, 北京 100123;
- 2. 中国合格评定国家认可中心, 北京 100062;
- 3. National Institute for Public Health and the Environment, Bilthoven 3721, The Netherlands
摘要: 为了研究低剂量的全氟辛烷磺酸(perfluorooctane sulfonate,PFOS)对水生生物的内分泌干扰效应和作用机制,考察了PFOS对斑马鱼(Brachydanio rerio)血浆和组织匀浆中卵黄蛋白原(vitellogenin,VTG)含量的影响。将雄性和雌性斑马鱼分别暴露于0.1、1、10和100 μg·L-1的PFOS中进行21 d毒性实验,染毒结束后分别检测雄鱼和雌鱼的血浆、头尾组织匀浆液和全鱼匀浆液中的VTG含量。结果显示:(1) PFOS暴露可引起斑马鱼血浆、全鱼和头尾匀浆液中VTG含量的升高,VTG含量的排序为雌鱼(血浆 >> 全鱼匀浆 > 头尾匀浆) >> 雄鱼(血浆 > 全鱼匀浆 >> 匀浆);(2) PFOS暴露所引起的雄鱼血浆和头尾匀浆中VTG含量的升高与剂量呈负相关关系,暴露浓度为0.1 μg·L-1时与对照组相比有显著性差异(p < 0.05);(3)雌鱼血浆和头尾匀浆中VTG含量的升高与剂量呈倒U型曲线关系,暴露浓度为10 μg·L-1时与对照组相比有显著性差异(p < 0.05);(4)雄鱼和雌鱼的全鱼匀浆液中的VTG含量与对照组相比均无显著性差异。研究结果表明,PFOS暴露对斑马鱼的内分泌干扰作用明显,其毒性作用机制可能是类雌激素效应,血液和头尾匀浆液中VTG含量能够作为PFOS内分泌干扰效应评价的敏感生物标志物,但响应曲线可能因性别和组织部位的不同有所差异。
Effect of Perfluorooctane Sulfonate (PFOS) on Vitellogenin Levels in Blood Plasma and Tissue Homogenate of Zebrafish (Brachydanio rerio)
- 1. Chinese Academy of Inspection and Quarantine, Beijing 100123, China;
- 2. China National Accreditation Service for Conformity Assessment, Beijing 100062, China;
- 3. National Institute for Public Health and the Environment, Bilthoven 3721, The Netherlands
- Received Date:
2011-07-27
Fund Project:
Abstract: To study the endocrine disrupting effect and action mechanism of low dose of perfluorooctane sulfonate (PFOS) on aquatic organisms, the effect of PFOS exposure on vitellogenin (VTG) levels in the blood plasma and tissue homogenate of zebrafish (Brachydanio rerio) was investigated. Male and female zebrafish were separately exposed to PFOS (0.1, 1, 10 and 100 μg·L -1) for 21 days. VTG levels in the blood plasma, whole body homogenate and head&tail homogenate were analyzed at the end of PFOS exposure. Results showed that:(1) VTG levels in the blood plasma, whole body homogenate and head & tail homogenate of zebrafish increased after PFOS exposure. VTG levels followed the order of (plasma > > whole body homogenate > head&tail homogenate) in female zebrafish > > (plasma > whole body homogenate > > head&tail homogenate) in male zebrafish. (2) VTG levels in the plasma and head&tail homogenate of male zebrafish increased in a negative dose-response pattern, and VTG levels in 0.1 μg·L-1 PFOS exposure group showed significant difference compared with the control (p <0.05). (3) VTG levels in the plasma and head&tail homogenate of female zebrafish increased in an inverted U-shaped dose-response pattern, and VTG levels in 10 μg·L-1 PFOS exposure group were significantly different from those in the control (p <0.05). (4) VTG levels in the whole body homogenate increased but had no significant difference compared with the control. Therefore, it was indicated that PFOS exposure could be active on the endocrine system of zebrafish with the xenoestrogenic action mechanism, and that VTG levels in the blood plasma and head&tail homogenate of zebrafish can be as sensitive biomarkers for assessing endocrine disrupting effect of PFOS, with different responding patterns dependent on sexes and tissues.
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