口服纳米银对大鼠的生物有效性及其体内分布的研究

江媛媛; 党菲; 李敏; 周东美; 吴笛; 施维林; 朱雪峰

doi:10.7524/AJE.1673-5897.20160902002

1. 苏州科技大学环境科学与工程学院, 苏州 215009;

2. 中国科学院南京土壤研究所土壤环境与污染修复重点实验室, 南京 210008;

3. 南京医科大学公共卫生学院毒理系, 南京 210029;

4. 南京医科大学公共卫生学院江苏省医药兽药安全性评价与研究中心, 南京 210029

作者简介: 江媛媛(1990-),女,硕士研究生,研究方向为生态毒理效应,E-mail:syjiangyuanyuan@163.com

基金项目:

国家自然科学基金项目(No. 41430752, 31570515)；江苏省自然科学基金项目(BK20131041)；苏州市科技支撑计划项目(SS201421，SS201523)；江苏高校水处理技术与材料协同创新项目；苏州市环保科技项目(2015-3)

中图分类号: X171.5

Bioavailability and Distribution of Nanosilver after Oral Administration in Rats

1. School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China;

2. Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;

3. Department of Toxicology, School of Public Health, Nanjing Medical University, Nanjing 210029, China;

4. Safety Evaluation and Research Center of Medicine and Veterinary Medicine of Jiangsu Province, School of Public Health, Nanjing Medical University, Nanjing 210029, China

Fund Project:

摘要: 由于独特的抗菌特性，纳米银(AgNP)在诸多领域得到广泛应用，但是其生物有效性、动物组织分布及排出尚不清楚。将聚乙烯吡咯烷酮包被的AgNP溶液按照10 mg·kg^-1给雌性SD大鼠灌胃，采用ICP-MS检测SD大鼠组织、粪便及尿液中总银浓度。结果表明，AgNP通过小肠吸收后，可以通过血液循环快速分布在肝、肾、脾、肺、脑等靶器官。灌胃后1 h，大鼠各组织中总银浓度达到最大值(肝、肾、脾、肺、脑中银浓度分别为(0.29 ±0.13) mg·kg^-1、(0.23 ±0.04) mg·kg^-1、(0.17 ±0.05) mg·kg^-1、(0.11 ±0.01) mg·kg^-1、(0.06 ±0.02) mg·kg^-1)，之后银浓度随时间而降低，直至和对照组无显著性差异。在灌胃途径下，AgNP对SD大鼠的有效性为8.5%，且73%的AgNP是通过粪便的途径排出体外。

Abstract: Due to the unique antimicrobial properties, nanosilver (AgNP) are widely applied in many fields. However, its bioavailability, tissue distribution and excretion in rats are less known. Female Sprague-Dawley rats were treated by a single intragastic administration of 10 mg·kg^-1 polyvinylpyrrolidone-AgNPs, and rat tissues, feces and urine were collected periodically to analyze total Ag concentrations by ICP-MS. The results showed that after uptake by gastrointestine, AgNP was transported via the blood circulation and distributed to tissues such as liver, kidney, spleen, lung, and brain. At 1 h after AgNP exposure, total silver accumulation in those tissues peaked (i.e. 0.29 ±0.13 mg·kg^-1, 0.23 ±0.04 mg·kg^-1, 0.17 ±0.05 mg·kg^-1, 0.11 ±0.01 mg·kg^-1 and 0.06 ±0.02 mg·kg^-1 for liver, kidney, spleen, lung and brain, respectively), and declined to background levels over time. Our calculation showed that the bioavailability of AgNP to Sprague-Dawley rats via intragastric administration was 8.5%. Especially, 73% of the dosed AgNP was excreted via feces.

Key words:

口服纳米银对大鼠的生物有效性及其体内分布的研究

作者简介: 江媛媛(1990-),女,硕士研究生,研究方向为生态毒理效应,E-mail:syjiangyuanyuan@163.com
1. 苏州科技大学环境科学与工程学院, 苏州 215009;

2. 中国科学院南京土壤研究所土壤环境与污染修复重点实验室, 南京 210008;

3. 南京医科大学公共卫生学院毒理系, 南京 210029;

4. 南京医科大学公共卫生学院江苏省医药兽药安全性评价与研究中心, 南京 210029

收稿日期: 2016-09-02

基金项目:

关键词:

AgNP /
SD大鼠 /
累积 /
生物有效性

Bioavailability and Distribution of Nanosilver after Oral Administration in Rats

1. School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China;

2. Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;

3. Department of Toxicology, School of Public Health, Nanjing Medical University, Nanjing 210029, China;

4. Safety Evaluation and Research Center of Medicine and Veterinary Medicine of Jiangsu Province, School of Public Health, Nanjing Medical University, Nanjing 210029, China

Received Date: 2016-09-02

Fund Project:

Keywords:

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