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樊磊1,曹继军2,张英杰1,刘颖1,张治然1,袭荣刚1,王晓波1,*. 汽车尾气暴露对小鼠运动力竭时间的影响[J]. 生态毒理学报, 2017, 12(5): 287-293
汽车尾气暴露对小鼠运动力竭时间的影响
The Effect of Vehicle Exhaust Exposure on the Running Exhausting Time of Mice
投稿时间:2016-09-04  修订日期:2016-10-31
DOI:10.7524/AJE.1673-5897.20160904003
中文关键词:  汽车尾气  小鼠  运动力竭  肺损伤  氧化应激
英文关键词:vehicle exhaust  mice  running exhausting  pulmonary injury  oxidative stress
基金项目:中国博士后科学基金面上项目(2015M572801)
作者单位
樊磊1,曹继军2,张英杰1,刘颖1,张治然1,袭荣刚1,王晓波1,* 1. 中国人民解放军第210医院药学部大连 116021 2. 辽东学院丹东 118001 
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中文摘要:
      汽车尾气是城市中大气污染物的主要来源,为探究汽车尾气对机体的损伤作用及其机制,通过建立汽车尾气暴露动物模型,利用轮式疲劳仪评估汽车尾气对小鼠运动力竭时间的影响。结果表明,汽车尾气连续暴露30 d后,暴露组小鼠的运动力竭时间较正常对照组明显缩短,支气管-肺泡灌洗液(BALF)中细胞因子白介素-1β(IL-1β),白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)水平较正常对照组明显升高,血清中谷胱甘肽(GSH)和超氧化物歧化酶(SOD)活性明显降低,丙二醛(MDA)含量显著升高。汽车尾气长时间暴露组(8 h d-1)BALF中白细胞计数和中性粒细胞百分比较正常对照组明显升高。肺组织切片染色后可见汽车尾气长时间暴露组小鼠肺泡间质中有较多中性粒细胞及淋巴细胞浸润。上述研究结果表明,汽车尾气可能通过引起呼吸系统损伤和降低机体抗氧化应激能力减少小鼠的运动力竭时间。
  
AuthorAffiliation
Fan Lei1, Cao Jijun2, Zhang Yingjie1, Liu Ying1, Zhang Zhiran1, Xi Ronggang1, Wang Xiaobo1,*1. Department of Pharmacy, No. 210 Hospital of PLA, Dalian 116021, China 2. Eastern Liaoning University, Dandong 118001, China
英文摘要:
      The purpose of this research was to establish an animal model and to determine the effects of vehicle exhaust exposure on the running exhausting time of the model. 24 mice were divided into three groups randomly. Two vehicle exhaust exposure groups lived in the cages with high concentration of vehicle exhaust for 4 and 8 hours every day (4 h d-1 group and 8 h d-1 group),and the control group was supplied with fresh air. Exhaustion time of running was recorded before the mice were exposed to vehicle exhaust. After 30 days vehicle exhaust exposure, the running exhausting time of mice was recorded again. The effect of vehicle exhaust on the mice was measured by comparing the running exhausting time before and after vehicle exhaust exposure. After the running exhausting test, the mice were sacrificed, and the blood was collected to detect the levels of glutathione (GSH),superoxide dismutase (SOD) and malondialdehyde (MDA) in the serum. Bronchoalveolar lavage fluid (BALF) was got by injecting saline solution into mice airways, and redrawing the saline solution into the connected syringe. The BALF was then centrifuged, and the resuspended cell pellet was used to make cytologic test. The inflammatory cytokines in the supernatant of BALF were detected by enzyme-linked immunosorbent assay (ELISA). The lungs of mice were removed for pathological examination. After 30 days of vehicle exhaust exposure, the running exhausting time of mice were significantly reduced in the vehicle exhaust exposure groups (4 h d-1 group and 8 h d-1 group), and the 8 h d-1 group showed an exacerbation of the reduced running exhausting time. The serum activity of GSH and SOD was lower, and the content of MDA was higher than those of the control group. The levels of inflammatory factors TNF-α, IL-1β and IL-6 in the BALF were higher in the vehicle exhaust exposure groups. Cell count results showed that the leukocyte count and neutral granular cell ratio in the BALF were higher in the vehicle exhaust exposure groups compared to those of control group. The histopathological changes of lungs were showed as inflammatory cells infiltrated in the alveolar interstitial tissues after 30 days of vehicle exhaust exposure. The results showed vehicle exhaust can reduce exhaustion time of mice by causing respiratory damage and reducing the body's ability to resist oxidative stress.
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