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陈佳月,解静芳*,姜洪进,贾真真,刘瑞卿. 草甘膦与镉复合胁迫对玉米幼苗抗氧化酶活性及光合作用的影响[J]. 生态毒理学报, 2018, 13(1): 219-228
草甘膦与镉复合胁迫对玉米幼苗抗氧化酶活性及光合作用的影响
Effects of Glyphosate and Cadmium Combined Stress on Antioxidant Enzyme Activity and Photosynthesis of Maize Seedling
投稿时间:2017-09-15  修订日期:2017-11-07
DOI:10.7524/AJE.1673-5897.20170915001
中文关键词:  草甘膦  Cd  玉米幼苗  抗氧化酶  叶绿素含量  OJIP
英文关键词:glyphosate  cadmium  maize seedling  antioxidant enzymes  chlorophyll content  OJIP
基金项目:国家自然科学基金项目(No.30740037);国家公益性行业(农业)科研专项项目(No.201103024)
作者单位
陈佳月,解静芳*,姜洪进,贾真真,刘瑞卿 山西大学环境与资源学院太原 030006 
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中文摘要:
      为了探讨草甘膦(PMG)与重金属镉(Cd)复合胁迫对作物(玉米幼苗)生长的影响作用机制。通过温室盆栽试验,分别进行了不同浓度的PMG单一处理(浓度分别设计为0、1.25、2.5、5、10、20 mg kg-1)和不同浓度的PMG(浓度分别为0、1.25、2.5、5、10、20 mg kg-1)与浓度5 mg kg-1 Cd2+的复合处理。采用分光光度法和连续激发式荧光仪分别对玉米幼苗抗氧化酶(过氧化物酶POD、过氧化氢酶CAT、超氧化物歧化酶SOD)、丙二醛(MDA)、叶绿素含量、荧光动力学曲线及相关参数进行了测定。结果表明,单一和复合胁迫分别在PMG浓度为1.25~5 mg kg-1、1.25~2.5 mg kg-1时,玉米幼苗通过增大抗氧化酶(SOD、POD、CAT)活性,清除积累过多的活性氧自由基,提高叶绿素含量的合成,加大光合作用速率,促进玉米幼苗的生长;单一和复合胁迫分别在PMG浓度为5~20 mg kg-1、2.5~20 mg kg-1时,由于玉米幼苗积累了过多的膜脂过氧化物,导致抗氧化系统损坏,阻碍叶绿素含量的合成,同时也损害了PSII的功能(MOφPOΨOφEOφDO、ABS/RC、TRO/RC、ETO/RC、DIO/RC、PIABS),导致玉米幼苗光合作用受到抑制,阻碍幼苗的生长。研究表明,草甘膦单一胁迫和与重金属镉复合胁迫,对玉米幼苗酶活性及光合作用的影响,均随处理浓度的升高表现为双阶段性,低浓度促进,高浓度抑制;与同浓度的PMG单一处理相比,Cd2+的存在,加大了PMG单独存在时的损害作用,使得玉米幼苗对PMG胁迫的敏感浓度点从5 mg kg-1降低到2.5 mg kg-1
  
AuthorAffiliation
Chen Jiayue, Xie Jingfang*, Jiang Hongjin, Jia Zhenzhen, Liu RuiqingCollege of Environment and Resource, Shanxi University, Taiyuan 030006, China
英文摘要:
      In order to investigate the underlying mechanisms of effects on crops growth (maize seedling) under the combined stress of glyphosate (PMG) and cadmium (Cd), the pot experiments in greenhouse were carried out, including a single treatment with one of the PMG concentration sets (0, 1.25, 2.5, 5, 10 and 20 mg kg-1) and a combined treatment together with one of the concentration sets of PMG (the concentration of 0, 1.25, 2.5, 5, 10, 20 mg kg-1, respectively) and 5 mg kg-1 of Cd2+. The maize seedling antioxidant enzymes (peroxidase POD, catalase CAT and superoxide dismutase SOD), malondialdehyde (MDA) content, chlorophyll fluorescence, dynamic curve and related parameters were determined by using spectrophotometry and a continuous excitation fluorometer. Under the condition of either the single exposure of PMG (1.25-5 mg kg-1) or the combined exposure (1.25-2.5 mg kg-1 PMG and Cd), the results showed that the activities of the antioxidant enzymes (SOD, POD, CAT) were increased in maize seedlings, the accumulation of reactive oxygen species were decreased, and the rates of the synthesis of chlorophyll and photosynthesis were also enhanced. In addition, under the single exposure of PMG (5-20 mg kg-1) or the combined exposure (2.5-20 mg kg-1 PMG and Cd), the contents of lipid peroxidation (MDA) in maize seedlings were higher than the other treatments, the antioxidant system were destroyed, and the synthesis of chlorophyll content were suppressed accompanied with the damaged functions of PSII (MO, φPO, ΨO, φEO, φDO, ABS/RC, TRO/RC, ETO/RC, DIO/RC, PIABS). All these changes finally restrained the photosynthesis of maize seedlings and inhibited the seedling growth. It was concluded that there were two phases for the effects of single PMG or combined with Cd stress on enzyme activity and photosynthesis in maize seedlings, in which a promotion at the low concentration and an inhibition at the high concentration. Compared with the single treatment, Cd2+ aggravated the damage of PMG treatment alone, and decreased the sensitive concentration of maize seedling to PMG stress from 5 mg kg-1 to 2.5 mg kg-1.
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