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-. 应用体内和体外方法评价原油水溶性组分和原油污染的地表水对淡水生物的内分泌干扰潜力[J]. 生态毒理学报, 2017, 12(3):
应用体内和体外方法评价原油水溶性组分和原油污染的地表水对淡水生物的内分泌干扰潜力
An Evaluation of the Endocrine Disruptive Potential of Crude Oil Water Accommodated Fractions and Crude Oil Contaminated Surface Water to Freshwater Organisms Using in vitro and in vivo Approaches
  
DOI:10.7524/AJE.1673-5897.201717etc
中文关键词:  
英文关键词:Endocrine disrupting chemical  Freshwater toxicology  Polycyclic aromatic hydrocarbon  Oil spill  Amphibian
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中文摘要:
      目前关于原油对淡水水生脊椎动物内分泌信号的潜在影响的认知有限。本文以非洲爪蟾(爪蟾属,X. laevis)蝌蚪和莫桑比克罗非鱼幼鱼(口孵非鲫属,O. mossambicus)为研究对象,选择风化原油和未风化提炼后的原油这2种原油的水溶性组分(WAFs)进行暴露实验,选择出可作为内分泌信号改变的生物标志物的基因,对其表达进行了研究。此外,将X. laevis蝌蚪暴露于靠近地下石油库的地表水中,对上述基因表达做了定量分析。利用重组酵母对原油、原油水溶性组分和地表水的(抗)雌激素和(抗)雄激素性进行了评价。在X. laevis对2种原油WAFs的响应中,甲状腺激素受体β表达均显著下调,而另一种与甲状腺相关的基因,2型脱碘酶,在O. mossambicus暴露于高浓度的原油WAF时被上调。此外,这2种WAFs都改变了与脂肪生成相关的过氧化物酶体增殖物激活受体γ在X. laevis中的表达。原油和WAFs在体外表现出抗雌激素和抗雄性激素的活性。然而,O. mossambicus雄激素受体2作为唯一一个代表生殖系统的基因,受到WAF暴露的影响显著。在地表水样品中发现了雌激素、抗雌激素和抗雄激素的作用;但是,在暴露于地表水的X. laevis中没有观察到任何基因表达的明显变化。在所用的2种模式生物以及2种原油中,响应各不相同。尽管如此,这些数据提供了证据证明原油污染可能会因内分泌信号转变对淡水鱼和两栖动物的健康造成不利影响。
精选自Truter, J. C., van Wyk, J. H., Oberholster, P. J., Botha, A.-M. and Mokwena, L. M. (2017), An evaluation of the endocrine disruptive potential of crude oil water accommodated fractions and crude oil contaminated surface water to freshwater organisms using in vitroand in vivo approaches. Environmental Toxicology and Chemistry, 36: 1330–1342. doi: 10.1002/etc.3665
详情请见http://onlinelibrary.wiley.com/doi/10.1002/etc.3665/full
  
AuthorAffiliation
J. Christoff Truter 1,*, Johannes H. van Wyk 1, Paul J. Oberholster 1,2, Anna-Maria Botha 3, Lucky M. Mokwena 41. Department of Botany and Zoology, Stellenbosch University, Stellenbosch, South Africa 2. CSIR Natural Resources and the Environment, Stellenbosch, South Africa 3. Department of Genetics, Stellenbosch University, Stellenbosch, South Africa 4. Central Analytical Facility, Mass Spectrometry Unit, University of Stellenbosch, Stellenbosch, South Africa
英文摘要:
      Knowledge regarding the potential impacts of crude oil on endocrine signaling in freshwater aquatic vertebrates is limited. The expression of selected genes as biomarkers for altered endocrine signaling was studied in African clawed frog,?Xenopus laevis, tadpoles and juvenile Mozambique tilapia,?Oreochromis mossambicus, exposed to weathered bunker and unweathered refinery crude oil water accommodated fractions (WAFs). In addition, the expression of the aforementioned genes was quantified in?X. laevis?tadpoles exposed to surface water collected from the proximity of an underground oil bunker. The (anti)estrogenicity and (anti)androgenicity of crude oil, crude oil WAFs, and surface water were furthermore evaluated using recombinant yeast. Thyroid hormone receptor beta expression was significantly down-regulated in?X. laevis?in response to both oil WAF types, whereas a further thyroid linked gene, type 2 deiodinase, was up-regulated in?O. mossambicus?exposed to a high concentration of bunker oil WAF. In addition, both WAFs altered the expression of the adipogenesis-linked peroxisome proliferator–activated receptor gamma in?X. laevis. The crude oil and WAFs exhibited antiestrogenic and antiandrogenic activity in vitro. However,?O. mossambicus?androgen receptor 2 was the only gene, representing the reproductive system, significantly affected by WAF exposure. Estrogenicity, antiestrogenicity, and antiandrogenicity were detected in surface water samples; however, no significant changes were observed in the expression of any of the genes evaluated in?X. laevis?exposed to surface water. The responses varied among the 2 model organisms used, as well as among the 2 types of crude oil. Nonetheless, the data provide evidence that crude oil pollution may lead to adverse health effects in freshwater fish and amphibians as a result of altered endocrine signaling.
View more in http://onlinelibrary.wiley.com/doi/10.1002/etc.3665/full
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