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-. OECD 236的鱼类胚胎实验(Fish Embryo Test, FET)是否足够敏感,能有效进行排水风险评价吗?[J]. 生态毒理学报, 2018, 13(5):
OECD 236的鱼类胚胎实验(Fish Embryo Test, FET)是否足够敏感,能有效进行排水风险评价吗?
Is it Fish Embryo Test (FET) according to OECD 236 sensible enough for delivering quality data for effluent risk assessment?
  
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中文摘要:
      近年来,由于“动物友好”协议的广泛应用,鱼类胚胎实验(Fish Embryo Test, FET)成为了生态毒理学研究的主要方法。OECD 236 FET方法在REACH法规所辖范围内广泛应用,主要针对简单混合物暴露情况(simple mixture exposures),但其还在世界范围内被盲目地用于排放废水综合测试(Whole Effluent Test, WET),这是一种复杂混合物暴露情况。然而,评价FET方法用于WET的有效性的可靠数据几乎没有。本文将OECD 236 FET方法与其他国际通用的标准的和略作改进的生态毒理学研究方案进行了比较,得出了可用于WET的测试方法的总体评价结果。我们使用了未经处理的医院排水,通常认为该排水具有高毒性,且会进入市政污水处理系统。我们选择的毒性测试方法包括OECD 203 (juvenile), USEPA 2000.0 (larva) and OECD 236 (embryo)。值得一提的是,我们还额外测试了3个亚致死指标(不移动性、不孵化率、心包囊肿),这些指标可以提高OECD 236方法的敏感性。我们在所选的8种方法中考察了急性毒性数据,方法的敏感性均有提高且胚胎实验≤仔鱼实验<幼鱼实验(Embryo≤Juvenile 详情请见 https://doi.org/10.1002/etc.4215
  
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Julio Alberto Alegre Stelzer, Catiusa Kuchak Rosin, Luana Hainzenreder Bauer, Marilia Hartmann, Fernando Hepp Pulgati, Alexandre Arenzon
英文摘要:
      On the past few years, Fish Embryo Test (FET) became protagonist regarding the usage of “animal-friendly” protocols for ecotoxicological proposes. As OECD 236 FET has been widely applied on the scope of REACH (simple mixture exposures), but it is blindly worldwide spreading as a Whole Effluent Test (WET) - complex mixture exposures. However, comparative peer-reviewed data regarding FET’s efficiency for WET is virtually absent. The primary objective of this paper is to compare OECD 236 with other worldwide used standard and slightly modified ecotoxicological protocols, providing a comprehensive overview of available tests for WET. For this, we used an untreated hospital effluent considered to be highly toxic but disposed on municipal sewerage. Base methods were OECD 203 (juvenile), USEPA 2000.0 (larva) and OECD 236 (embryo). Complimentarily, we evaluated the addition of three costless sublethal metrics (Immobility, non-hatching, and pericardial edema) that could enhance OECD 236 sensitivity. As result we observed acute toxicity in all 8 tested methodologies, with a clear escalation in sensitivity (Embryo ≤ Juvenile < Larvae). Larval test were the most sensitive tests for WET. Nevertheless, the addition of sublethal metrics to OECD 236 showed a positive outcome enhancing OECD 236 sensitivity in over 30%. Thus, we conclude that OECD 236 acts below its potential and FET may not be the most sensitive life stage for WET.
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