摘要:
为了探明磺胺甲恶唑(sulfamethoxazole,SMZ)的生态毒理效应,通过实验室人工控制毒理实验,研究了低浓度SMZ暴露对小麦体内超氧化物歧化酶(SOD)活性、叶绿素(CHL)和蛋白质(SP)含量的影响。结果显示:染毒7d后,各浓度组小麦叶片的SOD活性均被显著诱导(P<0.01),并且染毒浓度的升高增强了SOD的活性,表明SMZ暴露胁迫下,小麦可启动自身的保护机制以最大限度地减少自由基损伤。而小麦叶片的CHL含量,随着SMZ染毒浓度的增加而逐渐降低。并且当SMZ 暴露浓度较高时,小麦叶片的CHL含量被显著抑制(P<0.05))。当SMZ染毒浓度为0.05~0.50mg·L-1时,SP的含量被显著诱导(P<0.01));1.00mg·L-1SMZ对SP的含量产生显著(P<0.01)抑制,这说明SMZ染毒剂量将对蛋白质的合成产生严重影响。综上,SOD的活性变化可反映出SMZ暴露对小麦的污染效应及其生态毒性作用,但将其作为评估SMZ污染暴露的生物标志物有待于进一步深入研究。
Abstract:
To study the ecotoxicology of sulfamethoxazole (SMZ), the laboratory toxicity test was conducted to investigate the toxic effects of SMZ on the superoxide dismutases (SOD) activity and the contents of chlorophyll (CHL) and soluble protein (SP) of wheat (Triticum aestivum). The results showed that after the 7-day exposure, SOD activity in all the SMZ treatments was significantly (P<0.01) higher than that in the control. And the SOD activity increased with the increase in SMZ concentration. It is indicated that wheat could prevent the reactive oxygen species (ROS) damage by scavenging free radicals. While, the CHL content decreased with the increase in SMZ concentration. The treatments with higher SMZ concentrations significantly (P<0.05) decreased the CHL content in Triticum aestivum. The SP content in the treatments of 0.05-0.50mg·L-1 of SMZ was significantly (P<0.01) higher than that in the control. In contrast, the SP content in the treatment of 1.00mg·L-1 of SMZ decreased significantly (P<0.01). It is indicated that SMZ influenced the protein synthesis of wheat leaves. Therefore, the changes in the activity of SOD were able to reflect the toxic effects of pollutants on wheat. SOD as good biomarker for evaluating the ecotoxicological effect of SMZ exposure needs further study.
点击查看大图
下载:
