利用重组大肠杆菌SOS法评价污水厂出水的遗传毒性以及工艺去除效果
The Use of Recombinant E.coli SOS Test to Evaluate the Genotoxicity of Plant Sewage Water and the Efficiency of Contaminant Removal
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摘要: SOS/umu测试法被广泛应用于化合物和复杂混合物遗传毒性的评价，由于该技术所用菌种为致病菌且操作步骤繁琐等原因，制约了技术的推广应用。研究建立了基于重组大肠杆菌SOS效应的水质遗传毒性检测方法(专利号：ZL201110022476.1)，应用该方法评价了某市4座污水厂出水的直接遗传毒性效应，同时以污水处理一厂为例考察了直接遗传毒性效应的季节变化规律以及不同的工艺对水中直接遗传毒性物质的去除情况。结果显示：各污水厂出水均表现出一定的直接遗传毒性，对应的4-NQO毒性当量浓度范围为0.018~0.514 mg·L-1；一年四季中夏季进出水直接遗传毒性效应最高，现有工艺中生化处理工艺段对直接遗传毒性去除效果最佳，去除率为33.33%。该方法操作便利、检测敏感性较高、操作危险性较低，可用于水中直接遗传毒性效应的测试。Abstract: SOS/umu test is widely used in genotoxicity evaluation of bioactive compounds and complex mixtures. Because the strains used for testing are pathogenic and the test is a tedious process, its use in the evaluation of domestic products is limited. In this paper we established a method using recombinant E.coli SOS-fluorescence (patent number: ZL201110022476.1) to test the direct genetic toxicity of sewage water and evaluate the direct genotoxicity of treated water from four sewage plants. Moreover, the seasonal variation of direct genotoxicity and the influence of different processes on the removal of contaminants were evaluated in one plant. Positive reactions were found in all treated water, and the corresponding 4-NQO equivalent concentration was from 0.018 to 0.514 mg·L-1. The direct genotoxicity of raw and treated water was the highest in summer. The direct genetic toxicity removal effect reached 33.33% with the current biochemical treatment process. With convenient operation, high detection sensitivity, and low potential operational risk, this method can be used in water direct genotoxicity testing.
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Key words:
- wastewater /
- recombinant E.coli /
- SOS effect /
- genotoxic activity
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