摘要:
本文基于我国近海典型底栖双壳类生物毛蚶、紫贻贝急性和慢性毒性效应实验数据,分析了重金属汞对受试生物体质量、体长及消化腺超氧化物歧化酶(superoxide dismutase,SOD)酶活力的影响,获取了无观察效应浓度(No Observed Effect Concentration,NOEC),并以生存率为反应终点分析计算了重金属汞的非检测毒性效应浓度及半致死浓度。结果表明,各实验组SOD酶活力的组内方差与组间方差的差异整体上并不显著(P>0.05),实验周期内仅在个别时间处理组间存在显著性差异(P<0.05);实验周期内毛蚶和紫贻贝体质量、体长的组内方差与组间方差并没有显著性差异(P>0.1),体长及体质量不适合作为毒性效应的评价终点。重金属汞对毛蚶、紫贻贝的非检测毒性效应浓度分别为23.7 μg·L-1、87.8 μg·L-1,半致死浓度分别为683.4 μg·L-1、773.2 μg·L-1。
Abstract:
Acute and chronic toxic effects of mercury on Scapharca subcrenata and Mytilus edulis, typical coastal benthic bivalves of China, were studied. For the two bivalve groups, No Observed Effect Concentration (NOECs) of mercury were determined separately based on the three metrics of organisms being exposed, i.e., the weights, lengths and superoxide dismutase (SOD) activity. Non-detection toxic concentration and half lethal concentration were also obtained in this study. The results showed that except some test phases, there was no significant difference between intra-class variance and inter-class variance for SOD activity (P>0.05). For the weight and length, the significant difference between intra-class variance and inter-class variance were also not observed (P>0.1). Therefore, the length and weight cannot be used to evaluate the toxic effects at the end point. For Scapharca subcrenata and Mytilus edulis, non-detection toxic concentrations of mercury were 23.7 μg·L-1 and 87.8 μg·L-1, and half lethal concentrations for them were 683.4 μg·L-1 and 773.2 μg·L-1, respectively.