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磁铁矿纳米颗粒与Pb2+复合物对大鼠肾细胞的毒性研究

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陈力可, 郭昌胜, 刘淼, 李专, 徐建. 磁铁矿纳米颗粒与Pb2+复合物对大鼠肾细胞的毒性研究[J]. 生态毒理学报, 2020, 15(2): 87-95. doi: 10.7524/AJE.1673-5897.20190228004
引用本文: 陈力可, 郭昌胜, 刘淼, 李专, 徐建. 磁铁矿纳米颗粒与Pb2+复合物对大鼠肾细胞的毒性研究[J]. 生态毒理学报, 2020, 15(2): 87-95. doi: 10.7524/AJE.1673-5897.20190228004
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Chen Like, Guo Changsheng, Liu Miao, Li Zhuan, Xu Jian. The Cytotoxic Effects of Magnetite Nanoparticle-Pb2+ Complex on Rat Kidney Cells[J]. Asian Journal of Ecotoxicology, 2020, 15(2): 87-95. doi: 10.7524/AJE.1673-5897.20190228004
Citation: Chen Like, Guo Changsheng, Liu Miao, Li Zhuan, Xu Jian. The Cytotoxic Effects of Magnetite Nanoparticle-Pb2+ Complex on Rat Kidney Cells[J]. Asian Journal of Ecotoxicology, 2020, 15(2): 87-95. doi: 10.7524/AJE.1673-5897.20190228004
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磁铁矿纳米颗粒与Pb2+复合物对大鼠肾细胞的毒性研究

  • 1. 中国环境科学研究院, 环境健康风险评估与研究中心, 北京 100012;

  • 2. 吉林大学新能源与环境学院, 长春 130012;

  • 3. 吉林省环境监测中心站, 长春 130011

    • 作者简介: 陈力可(1984-),男,博士,研究方向为环境化学,E-mail:Downeychen@163.com
  • 基金项目:

    国家自然科学基金资助项目(51208482)

  • 中图分类号: X171.5

The Cytotoxic Effects of Magnetite Nanoparticle-Pb2+ Complex on Rat Kidney Cells

  • 1. Center of Environmental Health Risk Assessment and Research, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;

  • 2. College of New Energy and Environment, Jilin University, Changchun 130012, China;

  • 3. Environmental Monitoring Center of Jilin Province, Changchun 130011, China

  • Fund Project:

  • 摘要: 磁铁矿纳米颗粒(magnetite nanoparticles, MNPs)是一种环境友好型吸附剂，广泛应用于废水中Pb2+的处理。目前，有不少关于MNPs毒性的研究，但对MNPs处理Pb2+形成的复合物的毒性却鲜有报道，其复合毒性亟待深入研究。本文以大鼠肾细胞(NRK)作为细胞模型，系统研究不同Pb含量的MNPs-Pb复合物，以及相应浓度的MNPs和Pb2+，对大鼠肾细胞活性、细胞形态和密度的影响，考察细胞对纳米颗粒的摄取以及细胞凋亡的作用机制，评估MNPs-Pb的毒性效应。结果表明，在本实验浓度和暴露时间(12 h)条件下，Pb2+能够显著抑制细胞活性，改变细胞形态，促进细胞凋亡，对细胞有显著的毒性效应，并且呈现剂量相关性；利用MNPs吸附水环境中Pb2+形成的复合物MNPs-Pb对大鼠肾细胞没有显著的毒性作用(P<0.05)，MNPs对Pb2+的吸附可能是Pb2+的细胞毒性降低的原因。
    Abstract: Magnetite nanoparticles (MNPs) are environment-friendly adsorbents, which are widely used in the treatment of Pb2+ in wastewater. Recently, significant research efforts have been made toward the investigation of MNPs toxicity, whereas very little attention has been paid to the complex of MNPs and Pb2+ (MNPs-Pb). To evaluate the potential cytotoxic effect of MNPs-Pb, rat kidney cells (NRK) were used as model cell to study the cellular internalization of MNPs-Pb and the cytotoxic effects of both MNPs-Pb and Pb2+, including cell viability, cell morphology and density, and apoptosis. It was found that under the experimental concentrations and conditions, Pb2+ could significantly inhibit cell activity, change cell morphology and promote cell apoptosis, thus displaying dose-dependent toxic effects on cells. Compared with the corresponding concentration of Pb2+, the toxicity of MNPs-Pb complex on rat kidney cells was significantly reduced. There was no significant toxic effect of MNPs-Pb on rat kidney cells.
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  • 收稿日期:  2019-02-28

磁铁矿纳米颗粒与Pb2+复合物对大鼠肾细胞的毒性研究

    作者简介: 陈力可(1984-),男,博士,研究方向为环境化学,E-mail:Downeychen@163.com
  • 1. 中国环境科学研究院, 环境健康风险评估与研究中心, 北京 100012;
  • 2. 吉林大学新能源与环境学院, 长春 130012;
  • 3. 吉林省环境监测中心站, 长春 130011
  • 收稿日期:  2019-02-28
基金项目:

国家自然科学基金资助项目(51208482)

摘要: 磁铁矿纳米颗粒(magnetite nanoparticles, MNPs)是一种环境友好型吸附剂，广泛应用于废水中Pb2+的处理。目前，有不少关于MNPs毒性的研究，但对MNPs处理Pb2+形成的复合物的毒性却鲜有报道，其复合毒性亟待深入研究。本文以大鼠肾细胞(NRK)作为细胞模型，系统研究不同Pb含量的MNPs-Pb复合物，以及相应浓度的MNPs和Pb2+，对大鼠肾细胞活性、细胞形态和密度的影响，考察细胞对纳米颗粒的摄取以及细胞凋亡的作用机制，评估MNPs-Pb的毒性效应。结果表明，在本实验浓度和暴露时间(12 h)条件下，Pb2+能够显著抑制细胞活性，改变细胞形态，促进细胞凋亡，对细胞有显著的毒性效应，并且呈现剂量相关性；利用MNPs吸附水环境中Pb2+形成的复合物MNPs-Pb对大鼠肾细胞没有显著的毒性作用(P<0.05)，MNPs对Pb2+的吸附可能是Pb2+的细胞毒性降低的原因。

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