畜牧场中磺胺类抗生素及其抗性基因的空间分布规律
Spatial Distribution of Sulfonamides and Its Antibiotic Resistance Genes in Livestock Farm
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摘要: 为了解畜牧场内磺胺类抗生素及抗性基因的污染物空间分布情况、分布规律及相关性，选取某畜牧场的10 cm和50 cm深度的土壤样品共20个及5个粪便样品，分别对样品中13种磺胺类抗生素的浓度和3种磺胺类抗性基因（sul1、sul2和sul3）的丰度进行定量分析，并用地理信息系统优化采样并对污染物空间分布进行可视化分析。结果表明，垂直浓度的空间分布规律为粪便 > 10 cm深度土壤 > 50 cm深度土壤，水平浓度的空间分布主要集中在畜牧场的东南部。3种抗性基因在10 cm和50 cm深度土层中均有检出，其基因相对丰度分布情况为10 cm深度土壤>50 cm深度土壤，且抗生素对抗性基因的表达压力相似。本研究为抗生素和抗性基因污染的精准分析和深入研究畜牧场周边环境中抗性基因的传播规律提供基础数据。
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关键词:
- 磺胺类抗生素 /
- 抗生素抗性基因 /
- 畜牧场 /
- 液相色谱-串联质谱法 /
- 实时荧光定量PCR法
Abstract: In order to analyze the spatial distribution and correlation of sulfonamides (SAs) and their antibiotic resistance genes (ARGs), a livestock farm was taken as a case study, and 20 soil samples including 10-cm and 50-cm depth and 5 manure samples were collected. 13 kinds of SAs and 3 ARGs (sul1, sul2 and sul3) were quantitatively detected respectively, and Geographic Information System (GIS) was used to optimize sampling and visualize the spatial distribution. The spatial distribution of vertical concentrations were as followed:manure > 10 cm-depth soil > 50 cm-depth soil. The spatial distribution of horizontal concentrations were mainly concentrated in the southeast. 3 ARGs were detected in 10-cm and 50-cm depth soil layers. The distribution of ARGs relative abundance was 10 cm-depth soil> 50 cm-depth soil, and the expression pressure of antibiotic antagonistic genes was similar. It provides basic data for accurate analysis of antibiotic and resistance gene pollution and in-depth study of the spread of resistance genes in the surrounding environment of the livestock farms. -
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