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重组人金属硫蛋白-Ⅲα短肽毒性效应的初步研究

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甘俊英, 秦建新, 原海亮, 李强, 薛玉英. 重组人金属硫蛋白-Ⅲα短肽毒性效应的初步研究[J]. 生态毒理学报, 2021, 16(2): 227-234. doi: 10.7524/AJE.1673-5897.20200404002
引用本文: 甘俊英, 秦建新, 原海亮, 李强, 薛玉英. 重组人金属硫蛋白-Ⅲα短肽毒性效应的初步研究[J]. 生态毒理学报, 2021, 16(2): 227-234. doi: 10.7524/AJE.1673-5897.20200404002
shu
Gan Junying, Qin Jianxin, Yuan Hailiang, Li Qiang, Xue Yuying. Preliminary Study on Toxic Effects of Recombinant Human Metallothionein Ⅲ-α Peptide[J]. Asian Journal of Ecotoxicology, 2021, 16(2): 227-234. doi: 10.7524/AJE.1673-5897.20200404002
Citation: Gan Junying, Qin Jianxin, Yuan Hailiang, Li Qiang, Xue Yuying. Preliminary Study on Toxic Effects of Recombinant Human Metallothionein Ⅲ-α Peptide[J]. Asian Journal of Ecotoxicology, 2021, 16(2): 227-234. doi: 10.7524/AJE.1673-5897.20200404002
shu

重组人金属硫蛋白-Ⅲα短肽毒性效应的初步研究

  • 1. 环境医学工程教育部重点实验室, 东南大学公共卫生学院, 苏州纳米科技协同创新中心, 江苏省生物材料与器件重点实验室, 南京 210009;

  • 2. 苏州汇涵医用科技发展有限公司, 常熟 215500;

  • 3. 常熟市市场监督管理局, 常熟 215500

    • 作者简介: 甘俊英(1992-),女,硕士研究生,研究方向为纳米毒理学,E-mail:154912630@qq.com
    通讯作者: 薛玉英, E-mail: yyxue@seu.edu.cn
  • 基金项目:

    国家自然科学基金资助项目（81573186）；江苏省普通高校研究生实践创新计划资助项目（SJCX17_0067，SJCX18_0078）

  • 中图分类号: X171.5

Preliminary Study on Toxic Effects of Recombinant Human Metallothionein Ⅲ-α Peptide

  • 1. Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, School of Public Health, Southeast University, Collaborative Innovation Center of Suzhou Nano Science and Technology, Jiangsu Key Laboratory for Biomaterials and Devices, Nanjing 210009, China;

  • 2. Suzhou Huihan Medical Technology Development Co. Ltd., Changshu 215500, China;

  • 3. Changshu Municipal Market Supervision Administration, Changshu 215500, China

    • Corresponding author: Xue Yuying, yyxue@seu.edu.cn
  • Fund Project:

  • 摘要: 研究重组人金属硫蛋白Ⅲα短肽（rh-MT-Ⅲα）致HaCaT细胞、L929细胞和秀丽隐杆线虫（Caenorhabditis elegans，C. elegans）毒性效应，为rh-MT-Ⅲα的安全应用提供实验基础。细胞暴露于不同浓度的rh-MT-Ⅲα溶液（0~400 μg·mL-1），24 h后检测细胞存活率和乳酸脱氢酶（LDH）活性，观察细胞形态并检测细胞凋亡情况。结果表明，各剂量组2种细胞的存活率，与对照组相比均无显著性差异，细胞形态也未观察到显著变化。在400 μg·mL-1浓度下，LDH释放率明显增加，细胞凋亡率上升。线虫实验中，将线虫培养并同步化至L1期后进行染毒，剂量为5、50和500 μg·mL-1，暴露48 h后检测其运动行为（头部摆动和身体弯曲频率）、发育（体长）及摄食行为（咽泵频率），各指标均未有明显改变。研究表明，在一定的剂量水平下，rh-MT-Ⅲα对HaCaT细胞、L929细胞和秀丽隐杆线虫无明显毒性效应。
    Abstract: To study the toxic effects of recombinant human metallothionein Ⅲ-α peptide (rh-MT-Ⅲα) on HaCaT cells, L929 cells and nematode C. elegans, and provid experimental data for the safe application of rh-MT-Ⅲα, the cells were exposed to the rh-MT-Ⅲα solution at different doses (0~400 μg·mL-1) for 24 h and cell viability, release of lactate dehydrogenase (LDH), apoptosis and changes of cell morphology were measured. The results showed that there was no significant difference in cell viability and cell morphology between the rh-MT-Ⅲα treatment groups and the control when the administration doses range from 0 to 400 μg·mL-1. The release of LDH in cells treated with rh-MT-Ⅲα at 400 μg·mL-1 was higher than the control, which is consistent with the apoptosis caused by rh-MT-Ⅲα. The C. elegans were synchronized to the L1 phase and than exposed to the 5, 50, 500 μg·mL-1 rh-MT-Ⅲα for 48 h to detect the alternations in head swinging, body bending frequency, length and pumping rate. No difference were observed between the control and rh-MT-Ⅲα treatment groups. The findings suggested that the rh-MT-Ⅲα did not cause obvious toxic effects on HaCaT cells, L929 cells and C. elegans at a certain dose.
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  • 收稿日期:  2020-04-04
甘俊英, 秦建新, 原海亮, 李强, 薛玉英. 重组人金属硫蛋白-Ⅲα短肽毒性效应的初步研究[J]. 生态毒理学报, 2021, 16(2): 227-234. doi: 10.7524/AJE.1673-5897.20200404002
引用本文: 甘俊英, 秦建新, 原海亮, 李强, 薛玉英. 重组人金属硫蛋白-Ⅲα短肽毒性效应的初步研究[J]. 生态毒理学报, 2021, 16(2): 227-234. doi: 10.7524/AJE.1673-5897.20200404002
shu
Gan Junying, Qin Jianxin, Yuan Hailiang, Li Qiang, Xue Yuying. Preliminary Study on Toxic Effects of Recombinant Human Metallothionein Ⅲ-α Peptide[J]. Asian Journal of Ecotoxicology, 2021, 16(2): 227-234. doi: 10.7524/AJE.1673-5897.20200404002
Citation: Gan Junying, Qin Jianxin, Yuan Hailiang, Li Qiang, Xue Yuying. Preliminary Study on Toxic Effects of Recombinant Human Metallothionein Ⅲ-α Peptide[J]. Asian Journal of Ecotoxicology, 2021, 16(2): 227-234. doi: 10.7524/AJE.1673-5897.20200404002
shu

重组人金属硫蛋白-Ⅲα短肽毒性效应的初步研究

    通讯作者: 薛玉英, E-mail: yyxue@seu.edu.cn
    作者简介: 甘俊英(1992-),女,硕士研究生,研究方向为纳米毒理学,E-mail:154912630@qq.com
  • 1. 环境医学工程教育部重点实验室, 东南大学公共卫生学院, 苏州纳米科技协同创新中心, 江苏省生物材料与器件重点实验室, 南京 210009;
  • 2. 苏州汇涵医用科技发展有限公司, 常熟 215500;
  • 3. 常熟市市场监督管理局, 常熟 215500
  • 收稿日期:  2020-04-04
基金项目:

国家自然科学基金资助项目（81573186）；江苏省普通高校研究生实践创新计划资助项目（SJCX17_0067，SJCX18_0078）

摘要: 研究重组人金属硫蛋白Ⅲα短肽（rh-MT-Ⅲα）致HaCaT细胞、L929细胞和秀丽隐杆线虫（Caenorhabditis elegans，C. elegans）毒性效应，为rh-MT-Ⅲα的安全应用提供实验基础。细胞暴露于不同浓度的rh-MT-Ⅲα溶液（0~400 μg·mL-1），24 h后检测细胞存活率和乳酸脱氢酶（LDH）活性，观察细胞形态并检测细胞凋亡情况。结果表明，各剂量组2种细胞的存活率，与对照组相比均无显著性差异，细胞形态也未观察到显著变化。在400 μg·mL-1浓度下，LDH释放率明显增加，细胞凋亡率上升。线虫实验中，将线虫培养并同步化至L1期后进行染毒，剂量为5、50和500 μg·mL-1，暴露48 h后检测其运动行为（头部摆动和身体弯曲频率）、发育（体长）及摄食行为（咽泵频率），各指标均未有明显改变。研究表明，在一定的剂量水平下，rh-MT-Ⅲα对HaCaT细胞、L929细胞和秀丽隐杆线虫无明显毒性效应。

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