室内灰尘中五溴联苯醚(BDE-99)干扰Dio3影响斑马鱼甲状腺功能调节
BDE-99 from Indoor Dust Interferes with Dio3 and Affects Thyroid Function Regulation in Zebrafish
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摘要: 多溴联苯醚（PBDEs）是一种溴化阻燃剂，在办公室和家庭的灰尘中均有检出。PBDEs会对内分泌造成干扰，主要影响甲状腺激素的调节功能。使用斑马鱼胚胎为动物模型，评价办公室和家庭灰尘萃取物以及BDE-99对斑马鱼胚胎的影响。结果显示，灰尘萃取物和BDE-99同样导致斑马鱼眼部色素降低，GC-MS检测发现灰尘中含有BDE-99和BDE-47，且BDE-99含量远高于BDE-47，通过评估BDE-99在斑马鱼体内代谢及其对甲状腺激素脱碘酶Ⅲ（Dio3）表达的影响，发现Dio3在斑马鱼胚胎原肾管部位表达，且表达量显著增加，72 hpf斑马鱼胚胎的甲状腺素含量显著降低。研究表明，BDE-99对斑马鱼胚胎早期Dio3的活性有干扰作用，并导致甲状腺素含量降低，由此可知，斑马鱼胚胎色素变化和Dio3 mRNA表达有可能作为PBDEs的生物学评价指标。Abstract: PBDEs are brominated flame retardants that have been detected in dust in offices and homes. PBDEs can cause endocrine disruption by mainly affecting the regulation of thyroid hormones. This study used zebrafish embryos as a test species to evaluate the effects of the extracts of office and household dust and BDE-99 on zebrafish embryos. The results showed that dust extracts and BDE-99 both caused a decrease in the pigmentation of the zebrafish eyes. GC-MS analysis found that BDE-99 and BDE-47 were contained in the dust and the content of BDE-99 was much higher than BDE-47. By evaluating the effects of BDE-99 on zebrafish metabolism and the expression of the thyroid hormone deiodinase Ⅲ (Dio3), it was found that the expression of Dio3 was significantly enhanced in the protorenal duct of the zebrafish embryo. The thyroid hormone content in the 72 hpf zebrafish embryo was significantly reduced. The above results show that BDE-99 interferes with the activity of Dio3 in zebrafish embryos and leads to a decrease in thyroxine content. This study indicates that alterations in pigment of the zebrafish embryo and Dio3 mRNA expression may be used as biological indicators of PBDEs.
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Key words:
- PBDEs /
- dust /
- zebrafish embryos /
- Dio3 /
- BDE-99
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