水生生物毒性试验在环境损害司法鉴定领域的应用与发展
Application and Development of Aquatic Toxicity Test in Environmental Forensics
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摘要: 近年来,随着环境损害案件日益增多,我国生态环境损害赔偿制度日渐成熟,政府打击生态环境破坏案件的力度也在不断增大,环境损害司法鉴定意见的重要性和必要性日益凸显。如何选择科学、全面的判定方法以确保环境损害司法鉴定的科学性和公正性?这逐渐成为法庭毒物学和生态环境领域的研究热点。在现有环境损害案件中,水污染案件占据重要部分。水生生物毒性评价试验对环境损害司法鉴定意见(尤其是水体污染案件)的形成具有重要的生态毒理学意义。通过水生生物毒性试验可以综合、全面地分析污染物对生态环境以及生命体的影响。本文从环境损害司法鉴定实践应用角度出发,对发光细菌、藻类、溞类、鱼类以及大型甲壳类等《生态环境损害鉴定评估技术指南》推荐的水生模式生物的优缺点进行了系统性总结,并对不同的水生生物毒性评价方法在生态环境常见污染物毒性试验中应用概况进行了综述,旨在为我国环境损害司法鉴定水生生物毒性的研究和判定提供借鉴和参考。Abstract: In recent years, along with the growing number of environmental damage cases, China's ecological damage compensation system is becoming more mature, and Chinese Authorities have been increasing their efforts to prosecute offenders that cased ecological and environmental damages, which leads to the emerging importance and necessity of environmental forensic services. The issue as to how to choose a scientific and comprehensive determination method to ensure the scientificity and fairness of judicial appraisal of environmental damage, has gradually become a research hotspot in the field of forensic toxicology as well as ecological environment. Among the existing environmental damage cases, water pollution cases occupy an important part. The aquatic toxicity evaluation test has an important ecotoxicological significance to the formation of environmental forensic reports (especially for water pollution cases). This method can be used to analyze the impact of pollutants on the ecological environment and living organisms in an integrated and comprehensive manner. From the practical perspective, this paper summarized the advantages and disadvantages of the aquatic model organisms recommended in the Technical Guidelines for Identification and Evaluation of Ecological Damage, such as luminescent bacteria, algae, Daphnia magna, fish and macro-crustaceans, and reviewed the applications of different aquatic toxicity evaluation methods that have been used in the toxicity tests of common pollutants in the ecological environment. It aims to provide references for the analysis and determination of aquatic organism toxicity for judicial appraisal in China.
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张晓娜. 《环境损害司法鉴定白皮书》发布[N]. 民主与法制时报, 2021-06-08(1) 王元凤, 王旭, 王灿发, 等. 我国环境损害司法鉴定的现状与展望[J]. 中国司法鉴定, 2017(4):8-15 Wang Y F, Wang X, Wang C F, et al. Current situation and prospect of forensic appraisal of environmental damage in China[J]. Chinese Journal of Forensic Sciences, 2017 (4):8-15(in Chinese)
最高人民法院, 最高人民检察院, 司法部. 最高人民法院、最高人民检察院、司法部关于将环境损害司法鉴定纳入统一登记管理范围的通知[EB/OL]. (2015-12-21)[2021-11-20]. https://www.pkulaw.com/chl/afa74ed853e56022bdfb.html 司法部, 生态环境部. 司法部、生态环境部关于印发《环境损害司法鉴定执业分类规定》的通知.[EB/OL]. (2019-05-09)[2021-11-20]. https://www.pkulaw.com/chl/1f99294d9f2e98c2bdfb.html 《环境科学大辞典》编辑委员会. 环境科学大辞典[M]. 北京:中国环境科学出版社, 1991:184, 583, 629-631 陈和秋. 最高法发布环境资源典型案例[N]. 民主与法制时报, 2021-06-09(1) 中华人民共和国生态环境部, 国家市场监督管理总局. 生态环境损害鉴定评估技术指南总纲和关键环节第2部分:损害调查:GB/T 39791.2-2020[S]. 北京:中华人民共和国生态环境部, 国家市场监督管理总局, 2020 Cervi E C, Clark S, Boye K E, et al. Copper transformation, speciation, and detoxification in anoxic and suboxic freshwater sediments[J]. Chemosphere, 2021, 282:131063 Morcillo P, Esteban M Á, Cuesta A. Heavy metals produce toxicity, oxidative stress and apoptosis in the marine teleost fish SAF-1 cell line[J]. Chemosphere, 2016, 144:225-233 杨雅淇, 童一帆, 田胜艳. 三氯生在水生生态系统中的污染现状及其生物毒性效应[J]. 生态毒理学报, 2019, 14(6):47-56 Yang Y Q, Tong Y F, Tian S Y. Pollution status and toxic effect of triclosan in aquatic ecosystem[J]. Asian Journal of Ecotoxicology, 2019, 14(6):47-56(in Chinese)
朱英, 胡双庆, 沈根祥, 等. 2种典型家用消毒剂对水生生物的急性毒性影响及生态效应阈值研究[J]. 生态毒理学报, 2018, 13(4):161-169 Zhu Y, Hu S Q, Shen G X, et al. The study of acute toxicity effects on aquatic organisms and acute ecological threshold (PNECs) of two typical household disinfectants[J]. Asian Journal of Ecotoxicology, 2018, 13(4):161-169(in Chinese)
王作铭, 陈军, 陈静, 等. 地表水中抗生素复合残留对水生生物的毒性及其生态风险评价[J]. 生态毒理学报, 2018, 13(4):149-160 Wang Z M, Chen J, Chen J, et al. Toxicity to aquatic organisms and ecological risk assessment of antibiotic compound residues in the surface water[J]. Asian Journal of Ecotoxicology, 2018, 13(4):149-160(in Chinese)
丁婷婷, 董欣琪, 张瑾, 等. 3种氨基糖苷类抗生素对水生生物的时间依赖联合毒性作用比较[J]. 生态毒理学报, 2018, 13(1):126-137 Ding T T, Dong X Q, Zhang J, et al. Comparison of time-dependent joint toxicity interaction of three aminoglycosides antibiotics between two aquatic organisms[J]. Asian Journal of Ecotoxicology, 2018, 13(1):126-137(in Chinese)
Chapman P M. Whole effluent toxicity testing-Usefulness, level of protection, and risk assessment[J]. Environmental Toxicology and Chemistry, 2000, 19(1):3-13 王颖, 廖訚彧, 欧阳莎莉, 等. 发光细菌在线监测水体污染研究进展[J]. 净水技术, 2020, 39(9):10-16 Wang Y, Liao Y Y, Ouyang S L, et al. Research progress of on-line monitoring technology of water pollution by using luminescent bacteria[J]. Water Purification Technology, 2020, 39(9):10-16(in Chinese)
Chen Z L, Zhu Z H, Song J Y, et al. Linking biological toxicity and the spectral characteristics of contamination in seriously polluted urban rivers[J]. Environmental Sciences Europe, 2019, 31(1):1-10 韩丽君, 孙玉娟, 杨树平, 等. 发光细菌法测定SO2气体生物毒性的研究[J]. 农业与技术, 2020, 40(2):9-11 许春燕, 安晓雯, 王乐雪, 等. 发光细菌在环境生物毒性检测中的应用[J]. 辽宁化工, 2018, 47(3):256-258 Xu C Y, An X W, Wang L X, et al. Application of luminescent bacteria in the detection of environmental biological toxicity[J]. Liaoning Chemical Industry, 2018, 47(3):256-258(in Chinese)
朱文杰, 郑天凌, 李伟民. 发光细菌与环境毒性检测[M]. 北京:中国轻工业出版社, 2009:10-30 赵维超, 胡南, 丁德馨, 等. 低剂量γ辐射对青海弧菌Q67的毒性研究[J]. 辐射防护, 2017, 37(2):138-144 Zhao W C, Hu N, Ding D X, et al. Study on toxicity of low dose γ radiation to Qinghai Vibrio Q67[J]. Radiation Protection, 2017, 37(2):138-144(in Chinese)
江伟, 肖昕, 王昆, 等. 费氏弧菌检测土壤联合毒性模型分析[J]. 华侨大学学报:自然科学版, 2021, 42(6):809-816 Jiang W, Xiao X, Wang K, et al. Combined toxicity model analysis of soil detection with Vibrio fischeri[J]. Journal of Huaqiao University:Natural Science, 2021, 42(6):809-816(in Chinese)
彭良琼, 隆汶君, 何发明, 等. 发光细菌法评价坯革的生态毒性[J]. 皮革科学与工程, 2021, 31(6):1-7 Peng L Q, Long W J, He F M, et al. Evaluating ecotoxicity of leather by luminescent bacteria[J]. Leather Science and Engineering, 2021, 31(6):1-7(in Chinese)
何发明, 彭良琼, 林诗雨, 等. 发光细菌法评价常用有机鞣剂的生态毒性[J]. 皮革科学与工程, 2021, 31(3):14-18 , 29 He F M, Peng L Q, Lin S Y, et al. Assessment of ecotoxicity of common organic tanning agents by luminescent bacteria[J]. Leather Science and Engineering, 2021, 31(3):14-18, 29(in Chinese)
于鑫娅, 蒋鹏, 张华, 等. 石油烃污染场地的微波修复土壤急性毒性及生态风险评价[J]. 环境化学, 2021, 40(11):3413-3420 Yu X Y, Jiang P, Zhang H, et al. Assessment of acute soil toxicity and ecological risk in microwave remediation of petroleum hydrocarbon contaminated sites[J]. Environmental Chemistry, 2021, 40(11):3413-3420(in Chinese)
徐劲草, 张超萍. 基于微生物燃料电池(MFC)的水质生物毒性监测设备设计[J]. 低碳世界, 2021, 11(9):61-62 黄国兰, 戴树桂, 孙红文, 等. 有机污染物对藻类毒性的测定[J]. 环境化学, 1994, 13(3):259-262 Huang G L, Dai S G, Sun H W, et al. Determination of the toxicity of organic pollutants to algae[J]. Environmental Chemistry, 1994, 13(3):259-262(in Chinese)
胡丽, 陈敏, 殷高方, 等. 基于藻类光合作用抑制效应的敌草隆毒性响应参数研究[J]. 光谱学与光谱分析, 2021, 41(5):1519-1524 Hu L, Chen M, Yin G F, et al. Toxicity response parameters of diaquilone based on photosynthetic inhibition effect of algae[J]. Spectroscopy and Spectral Analysis, 2021, 41(5):1519-1524(in Chinese)
曾佳敏, 钟仕花, 钱伟, 等. 水环境中有机磷酸酯的污染现状及其生物毒性[J]. 中国环境科学, 2021, 41(9):4388-4401 Zeng J M, Zhong S H, Qian W, et al. Pollution status and ecotoxicity of organophosphate esters (OPEs) in aquatic environment[J]. China Environmental Science, 2021, 41(9):4388-4401(in Chinese)
林艳, 罗菲尔, 郭蔚华, 等. 藻红外技术测试多种卫生指标下的饮用水生物毒性[J]. 中国给水排水, 2019, 35(19):57-65 Lin Y, Luo F E, Guo W H, et al. Detection of biotoxicity for drinking water under multiple indexes by algae infrared technology[J]. China Water & Wastewater, 2019, 35(19):57-65(in Chinese)
欧泽奎, 刘东超, 谢恩义, 等. 海水环境中Hg2+、Cd2+、Pb2+对张氏马尾藻幼孢子体的生物毒性研究[J]. 南方水产科学, 2016, 12(5):43-52 Ou Z K, Liu D C, Xie E Y, et al. Biotoxcity of Hg2+, Cd2+ and Pb2+ in germlings of Sargassum zhangii Tseng et Lu in seawater[J]. South China Fisheries Science, 2016, 12(5):43-52(in Chinese)
宋大祥. 大型溞(Daphnia magna Straus)的初步培养研究[J]. 动物学报, 1962, 8(1):49-62 Song D X. Studies on the culturing of Daphnia magna Straus (Crustacea, Cladocera)[J]. Acta Zoologica Sinica, 1962, 8(1):49-62(in Chinese)
Bownik A. Effects of ectoine on behavioral, physiological and biochemical parameters of Daphnia magna exposed to dimethyl sulfoxide[J]. Science of the Total Environment, 2019, 683:193-201 尹东鹏, 赵文. 重金属对大型溞生态毒理学研究进展[J]. 大连海洋大学学报, 2020, 35(4):631-636 Yin D P, Zhao W. Ecotoxicological effects of heavy metals on water fleas Daphnia magna:A review[J]. Journal of Dalian Ocean University, 2020, 35(4):631-636(in Chinese)
Kim H Y, Lee M J, Yu S H, et al. The individual and population effects of tetracycline on Daphnia magna in multigenerational exposure[J]. Ecotoxicology, 2012, 21(4):993-1002 王京, 郑磊, 闫振广, 等. 代表性鲤科鱼类青鱼的物种敏感性评价[J]. 生态毒理学报, 2021, 16(2):270-276 Wang J, Zheng L, Yan Z G, et al. Species sensitivity evaluation of a Chinese representative cyprinid Mylopharyngodon piceus[J]. Asian Journal of Ecotoxicology, 2021, 16(2):270-276(in Chinese)
田华, 江国斌, 赵飞. 环境污染物对鱼类糖代谢的影响研究进展[J]. 海洋湖沼通报, 2021, 43(2):118-123 Tian H, Jiang G B, Zhao F. Research progresses in effects of environmental pollutants on glucose metabolism of fish species[J]. Transactions of Oceanology and Limnology, 2021, 43(2):118-123(in Chinese)
曾彬, 张存艳, 陈凯. 模式生物斑马鱼在药物毒性评价中的应用及研究现状[J]. 天然产物研究与开发, 2020, 32(10):1794-1801 Zeng B, Zhang C Y, Chen K. Application and research progress of model biological zebrafish in drug toxicity evaluation[J]. Natural Product Research and Development, 2020, 32(10):1794-1801(in Chinese)
Barbazuk W B, Korf I, Kadavi C, et al. The syntenic relationship of the zebrafish and human genomes[J]. Genome Research, 2000, 10(9):1351-1358 Howe K, Clark M D, Torroja C F, et al. The zebrafish reference genome sequence and its relationship to the human genome[J]. Nature, 2013, 496(7446):498-503 Embry M R, Belanger S E, Braunbeck T A, et al. The fish embryo toxicity test as an animal alternative method in hazard and risk assessment and scientific research[J]. Aquatic Toxicology, 2010, 97(2):79-87 He D F, Chen R R, Zhu E H, et al. Toxicity bioassays for water from black-odor rivers in Wenzhou, China[J]. Environmental Science and Pollution Research, 2015, 22(3):1731-1741 于彩虹, 周珂, 刘芸, 等. 城镇污水处理厂进出水对日本青鳉早期阶段亚慢性毒性效应[J]. 生态毒理学报, 2014, 9(1):56-62 Yu C H, Zhou K, Liu Y, et al. Subchronic toxicity effect of the wastewater and effluent from municipal sewage treatment plants on early life stage Japanese medaka (Oryzias latipes)[J]. Asian Journal of Ecotoxicology, 2014, 9(1):56-62(in Chinese)
刘存歧, 袁雅心, 王孟颖, 等. 城市污水处理厂尾水生物毒性研究[J]. 安全与环境学报, 2018, 18(6):2383-2389 Liu C Q, Yuan Y X, Wang M Y, et al. On the biological eco-toxicity of the effluent from the municipal sewage-treating plant[J]. Journal of Safety and Environment, 2018, 18(6):2383-2389(in Chinese)
李磊, 刘金殿, 黄士林, 等. 基于综合生物标志物响应评价苯并芘对脊尾白虾(Exopalaemon carinicauda)的毒性效应[J]. 海洋与湖沼, 2018, 49(2):444-451 Li L, Liu J D, Huang S L, et al. Integrated assessment of biomarker responses in Exopalaemon carinicauda exposed to benzo[a] pyrene[J]. Oceanologia et Limnologia Sinica, 2018, 49(2):444-451(in Chinese)
刘祥, 王敏, 陈求稳, 等. 典型重金属胁迫对日本沼虾的氧化损伤及交互作用[J]. 生态毒理学报, 2017, 12(6):116-126 Liu X, Wang M, Chen Q W, et al. Oxidative damage and interaction induced by typical heavy metals in waters on the Macrobranchium nipponense[J]. Asian Journal of Ecotoxicology, 2017, 12(6):116-126(in Chinese)
张颖, 余维初, 李嘉, 等. 非常规油气开发用滑溜水压裂液体系生物毒性评价实验研究[J]. 钻采工艺, 2020, 43(5):106-109 , 11 Zhang Y, Yu W C, Li J, et al. Experimental study on biological toxicity evaluation of slick water fracturing fluid system for unconventional oil and gas development[J]. Drilling & Production Technology, 2020, 43(5):106-109, 11(in Chinese)
周名江, 颜天, 李钧, 等. 黑褐新糠虾的急性毒性测试方法及在钻井液毒性评价中的作用[J]. 海洋环境科学, 2001, 20(3):1-4 Zhou M J, Yan T, Li J, et al. Acute toxicity test method using Neomysis awatschensis and its application in toxicity evaluation of drilling fluid[J]. Marine Environmental Science, 2001, 20(3):1-4(in Chinese)
Jia K, Ionescu R E. Measurement of bacterial bioluminescence intensity and spectrum:Current physical techniques and principles[J]. Advances in Biochemical Engineering/Biotechnology, 2016, 154:19-45 朱丽娜. 基于发光细菌法的水质综合毒性研究[D]. 北京:中央民族大学, 2012:1-12, 40-42 田程. 藻类荧光对重金属毒性响应规律的研究[D]. 石家庄:河北科技大学, 2010:6-13 Tian C. Study on the discipline of algal fluorescence response to heavy metal toxicity[D]. Shijiazhuang:Hebei University of Science and Technology, 2010:6 -13(in Chinese)
刘璐, 闫浩, 李诚, 等. 重金属铅与两种淡水藻的相互作用[J]. 生态学报, 2014, 34(7):1690-1697 Liu L, Yan H, Li C, et al. Interactions between heavy metal lead and two freshwater algae[J]. Acta Ecologica Sinica, 2014, 34(7):1690-1697(in Chinese)
Wu Y G, Lin C X, Yuan L. Characteristics of six cladocerans in relation to ecotoxicity testing[J]. Ecological Indicators, 2007, 7(4):768-775 李婧. 塑化剂邻苯二甲酸丁苄酯(BBP)对大型溞生物毒性的研究[D]. 保定:河北大学, 2020:1-19 Li J. Research on the biotoxicity of butyl benzyl phthalate (BBP)to Daphnia magna[D]. Baoding:Hebei University, 2020:1 -19(in Chinese)
刘祥, 王敏, 陈求稳, 等. 典型重金属胁迫对日本沼虾的氧化损伤及交互作用[J]. 生态毒理学报, 2017, 12(6):116-126 Liu X, Wang M, Chen Q W, et al. Oxidative damage and interaction induced by typical heavy metals in waters on the Macrobranchium nipponense[J]. Asian Journal of Ecotoxicology, 2017, 12(6):116-126(in Chinese)
吴满昌, 王嘎利, 胡学伟. 环境、健康与法律[M]. 北京:知识产权出版社, 2017:17-125 徐英江, 刘欢, 彭凯秀, 等. 水产品中三种常见重金属污染物的毒性及风险评估进展[J]. 现代食品科技, 2020, 36(9):314-324 Xu Y J, Liu H, Peng K X, et al. Progress in toxicity and risk assessment of three common heavy metal pollutants in aquatic products[J]. Modern Food Science and Technology, 2020, 36(9):314-324(in Chinese)
陈速敏, 张元勋, 尚晶, 等. 基于青海弧菌Q67的镀锌废水土壤污染的生物毒性效应[J]. 法医学杂志, 2020, 36(4):445-452 Chen S M, Zhang Y X, Shang J, et al. Biological toxicity effects of soil pollution caused by galvanized wastewater based on Vibrio qinghaiensis sp.-Q67[J]. Journal of Forensic Medicine, 2020, 36(4):445-452(in Chinese)
胡冰, 王华, 张冬冬, 等. 纳米TiO2和Cu(Ⅱ)、Zn(Ⅱ)对小球藻和新月菱形藻的毒性研究[J]. 大连海洋大学学报, 2015, 30(5):489-493 Hu B, Wang H, Zhang D D, et al. Toxicity of TiO2 nanoparticles, Cu(Ⅱ) and Zn(Ⅱ) to Chlorella sp. and Nitzschia closterium[J]. Journal of Dalian Ocean University, 2015, 30(5):489-493(in Chinese)
王宇, 黄宜宁, 刘子陶, 等. 模式生物斑马鱼在重金属神经发育毒性的应用进展[J]. 毒理学杂志, 2021, 35(4):340-345 熊小琴, 罗思, 吴本丽, 等. 不同硬度条件下Cd2+和Cu2+对稀有鮈鲫的急性毒性[J]. 生态毒理学报, 2016, 11(3):316-322 Xiong X Q, Luo S, Wu B L, et al. Acute toxicity of cadmium and copper to Gobiocypris rarus under different water hardness[J]. Asian Journal of Ecotoxicology, 2016, 11(3):316-322(in Chinese)
Bjerselius R, Winberg S, Winberg Y, et al. Ca2+ protects olfactory receptor function against acute Cu(Ⅱ) toxicity in Atlantic salmon[J]. Aquatic Toxicology, 1993, 25(1-2):125-137 Zhang J J, Jia J M, Ma Y H, et al. Emergent monitoring on some heavy metal and pesticide in drinking water using luminescent bacteria[J]. Journal of Preventive Medicine of Chinese People's Liberation Army, 2013, 31(1):9-11 仇爱锋, 王玉涛, 张树秋, 等. 克百威、镉和铜对费氏弧菌的联合毒性效应[J]. 农业环境科学学报, 2017, 36(5):869-875 Qiu A F, Wang Y T, Zhang S Q, et al. Joint toxic effects of carbofuran, Cd and Cu to Vibrio fischeri[J]. Journal of Agro-Environment Science, 2017, 36(5):869-875(in Chinese)
刘顺亮, 陶峰, 宋晓红, 等. 南方某铀矿山废水对生物的急性毒性研究[J]. 生态毒理学报, 2018, 13(4):203-208 Liu S L, Tao F, Song X H, et al. Study on the biological toxicity assessment of one southern uranium mine[J]. Asian Journal of Ecotoxicology, 2018, 13(4):203-208(in Chinese)
蔡卓平, 刘伟杰, 骆育敏, 等. 重金属镉和铅胁迫对海洋微藻的毒性效应研究[J]. 生态科学, 2019, 38(3):211-217 Cai Z P, Liu W J, Luo Y M, et al. Toxicity of Cd2+ and Pb2+ to the growth and physiology of marina microalga Karenia mikimotoi[J]. Ecological Science, 2019, 38(3):211-217(in Chinese)
胡丽, 殷高方, 赵南京, 等. 蛋白核小球藻光合荧光参数对Cu2+毒性响应特性研究[J]. 光学学报, 2020, 40(12):152-158 Hu L, Yin G F, Zhao N J, et al. Response of photosynthetic fluorescence parameters of Chlorella pyrenoidosa to Cu2+ toxicity[J]. Acta Optica Sinica, 2020, 40(12):152-158(in Chinese)
徐冬梅, 柯薇, 王彦华. 铜与毒死蜱复合污染对淡水绿藻的毒性效应[J]. 中国环境科学, 2018, 38(11):4348-4353 Xu D M, Ke W, Wang Y H. Toxic effects of the co-exposure to Cu and chlorpyrifos toward the freshwater green algae[J]. China Environmental Science, 2018, 38(11):4348-4353(in Chinese)
葸玉琴, 赖金霞, 张明旭, 等. Cr3+和Cd2+对普通小球藻生长及抗氧化酶活性的影响[J]. 微生物学报, 2021, 61(7):2091-2100 Xi Y Q, Lai J X, Zhang M X, et al. Effects of Cr3+ and Cd2+ on the growth and antioxidant enzyme activity of Chlorella vulgaris[J]. Acta Microbiologica Sinica, 2021, 61(7):2091-2100(in Chinese)
陈丽萍, 吴长兴, 苍涛, 等. 3种重金属离子对大型溞的急性毒性效应[J]. 浙江农业科学, 2018, 59(1):46-48 , 50
郭君秀, 唐珏, 赵文, 等. 重金属Hg2+、Cd2+对西藏拟溞的急性毒性研究[J]. 生物学杂志, 2012, 29(3):14-16 Guo J X, Tang J, Zhao W, et al. Study on the acute toxic effects of Hg2+ and Cd2+ on Daphniopsis tibetana Sars[J]. Journal of Biology, 2012, 29(3):14-16(in Chinese)
Xiong D W, Fang T, Yu L P, et al. Effects of nano-scale TiO2, ZnO and their bulk counterparts on zebrafish:Acute toxicity, oxidative stress and oxidative damage[J]. Science of the Total Environment, 2011, 409(8):1444-1452 廖伟, 刘大庆, 冯承莲, 等. 不同水体硬度条件下Cu2+对不同生长阶段斑马鱼的毒性[J]. 环境工程, 2019, 37(12):71-74 , 166 Liao W, Liu D Q, Feng C L, et al. Toxicity of copper(Cu2+) to zebrafish at different life stages under different water hardness[J]. Environmental Engineering, 2019, 37(12):71-74, 166(in Chinese)
刘伟, 吴孝兵, 赵娟. 重金属Cu2+对锦鲫和日本沼虾的急性毒性研究[J]. 资源开发与市场, 2008, 24(10):868-870 Liu W, Wu X B, Zhao J. Study on acute toxicity of Cu2+ to crucian carp and Japan marsh shrimp[J]. Resource Development & Market, 2008, 24(10):868-870(in Chinese)
杨志艳, 薛雅芳, 徐永健. 4种重金属离子对海水青鳉胚胎发育及仔鱼的急性毒性研究[J]. 宁波大学学报:理工版, 2021, 34(5):9-15 Yang Z Y, Xue Y F, Xu Y J. Effects of four heavy metal ions on embryonic development and larval growth of Oryzias melastigma[J]. Journal of Ningbo University:Natural Science & Engineering Edition, 2021, 34(5):9-15(in Chinese)
宋维彦, 靳桂双, 毕伟伟, 等. 五种重金属离子对克氏原螯虾(Procambarus clarkiai)的急性毒性作用研究[J]. 激光生物学报, 2010, 19(2):206-211 Song W Y, Jin G S, Bi W W, et al. Studies on acute toxicity of five heavy metal ion on Porcambarus clarkia Girard[J]. Acta Laser Biology Sinica, 2010, 19(2):206-211(in Chinese)
于丰军. 铅和镉两种重金属对中华绒螯蟹的毒性效应研究[D]. 上海:华东师范大学, 2005:9-14 Yu F J. The study of cadmium and lead toxicity on the Chinese mitten-handed crab, Eriocheir sinensis[D]. Shanghai:East China Normal University, 2005:9 -14(in Chinese)
蒋瑞卿. 生物监测在水质预警中的应用:鱼类氰化物毒理试验[J]. 净水技术, 2018, 37(S1):39-42 Jiang R Q. Application of biological monitoring in water quality early warning:Toxicity test of cyanide on fish[J]. Water Purification Technology, 2018, 37(S1):39-42(in Chinese)
国家履行斯德哥尔摩公约工作协调组办公室. 中华人民共和国履行《关于持久性有机污染物的斯德哥尔摩公约》国家实施计划[M]. 北京:中国环境科学出版社, 2008:1-10 Wu D, Yan H Y, Shang M S, et al. Water eutrophication evaluation based on semi-supervised classification:A case study in Three Gorges Reservoir[J]. Ecological Indicators, 2017, 81:362-372 吕向立, 吴鹏, 朱艾嘉, 等. 城市污水厂污泥倾倒对海洋生物的毒性研究[J]. 生态毒理学报, 2017, 12(3):636-643 Lv X L, Wu P, Zhu A J, et al. Toxicity of municipal sewage sludge to marine organisms[J]. Asian Journal of Ecotoxicology, 2017, 12(3):636-643(in Chinese)
武毛妮, 葛媛, 朱艳芳. 生活污水的生物毒性检测及成因分析[J]. 环境科学与技术, 2021, 44(3):130-134 Wu M N, Ge Y, Zhu Y F. Biotoxicity test and cause analysis in domestic wastewater[J]. Environmental Science & Technology, 2021, 44(3):130-134(in Chinese)
孙晓怡, 王雅梅, 金福杰, 等. 用鱼类检测工业废水毒性的研究[J]. 辽宁城乡环境科技, 2001, 21(6):28-31 杨一鸣, 崔积山, 童莉, 等. 美国VOCs定义演变历程对我国VOCs环境管控的启示[J]. 环境科学研究, 2017, 30(3):368-379 Yang Y M, Cui J S, Tong L, et al. Evolution of the definition of volatile organic compounds in the United States and its implications for China[J]. Research of Environmental Sciences, 2017, 30(3):368-379(in Chinese)
环境保护部. 环境保护部公告2013年第31号关于发布《水泥工业污染防治技术政策》《钢铁工业污染防治技术政策》《硫酸工业污染防治技术政策》《挥发性有机物(VOCs)污染防治技术政策》等四项指导性文件的公告[EB/OL]. (2013-05-24 )[2021-11-12]. https://www.pkulaw.com/chl/166edee129781351bdfb.html
Wu X M, Fan Z H, Zhu X L, et al. Exposures to volatile organic compounds (VOCs) and associated health risks of socio-economically disadvantaged population in a "hot spot" in Camden, New Jersey[J]. Atmospheric Environment, 2012, 57:72-79 王丽琴, 李博伟, 黄宇, 等. 环境中挥发性有机物监测及分析方法[J]. 地球环境学报, 2016, 7(2):130-139 , 182 Wang L Q, Li B W, Huang Y, et al. The monitoring and analysis methods of volatile organic compounds in the ambient air[J]. Journal of Earth Environment, 2016, 7(2):130-139, 182(in Chinese)
杨永安, 张凯, 唐红军, 等. 水环境中挥发性有机物的监测方法[J]. 四川环境, 2014, 33(3):108-112 Yang Y A, Zhang K, Tang H J, et al. Monitoring methods of volatile organic compounds in the water environment[J]. Sichuan Environment, 2014, 33(3):108-112(in Chinese)
刘东红, 陶玉强, 周文佐. 持久性有机污染物在中国湖泊生物中分布与富集的研究进展[J]. 湖泊科学, 2018, 30(3):581-596 Liu D H, Tao Y Q, Zhou W Z. Distribution and accumulation of persistent organic pollutants in aquatic organisms of Chinese Lakes[J]. Journal of Lake Sciences, 2018, 30(3):581-596(in Chinese)
王梦圆, 张龙飞, 汤云瑜, 等. 几种水生模式生物在持久性有机污染物毒理学评价中的研究进展[J]. 环境化学, 2021, 40(5):1361-1378 Wang M Y, Zhang L F, Tang Y Y, et al. Research progress of several aquatic biological models in toxicological evaluation of persistent organic pollutants[J]. Environmental Chemistry, 2021, 40(5):1361-1378(in Chinese)
Hill A J, Teraoka H, Heideman W, et al. Zebrafish as a model vertebrate for investigating chemical toxicity[J]. Toxicological Sciences, 2005, 86(1):6-19 Xu H Y, Li C X, Suklai P, et al. Differential sensitivities to dioxin-like compounds PCB 126 and PeCDF between Tg (cyp1a:Gfp) transgenic medaka and zebrafish larvae[J]. Chemosphere, 2018, 192:24-30 Flaherty C M, Dodson S I. Effects of pharmaceuticals on Daphnia survival, growth, and reproduction[J]. Chemosphere, 2005, 61(2):200-207 刘冉, 曹志会, 赵月, 等. PFOA和PFOS对大型蚤急性毒性试验研究[J]. 安全与环境工程, 2015, 22(4):51-55 , 74 Liu R, Cao Z H, Zhao Y, et al. Experiment study on acute toxicity of PFOA and PFOS to Daphnia magna[J]. Safety and Environmental Engineering, 2015, 22(4):51-55, 74(in Chinese)
赵培强, 董四君. 卤虫属在水生生态毒理学中的研究进展[J]. 生态毒理学报, 2020, 15(4):33-44 Zhao P Q, Dong S J. Advances in the research on Artemia in aquatic ecotocicology[J]. Asian Journal of Ecotoxicology, 2020, 15(4):33-44(in Chinese)
黄月娥, 李朝品, 张楠. 5种重金属暴露对秀丽线虫生物毒性效应效应的Meta回归分析[J]. 中国血吸虫病防治杂志, 2016, 28(2):172-177 Huang Y E, Li C P, Zhang N. Meta regression analysis of five heavy metal biotoxicity effects on Caenorhabditis elegans[J]. Chinese Journal of Schistosomiasis Control, 2016, 28(2):172-177(in Chinese)
张汉文, 林家进, 黄月娥, 等. 基于秀丽线虫的5种重金属生物毒性评价[J]. 环境与健康杂志, 2018, 35(10):890-892 Zhang H W, Lin J J, Huang Y E, et al. Biotoxicity of five heavy metals to Caenorhabditis elegans[J]. Journal of Environment and Health, 2018, 35(10):890-892(in Chinese)
孙成华, 刘保献, 鹿海峰, 等. 细颗粒物(PM2.5)主要组分模拟溶液对发光细菌的光抑制分析[J]. 生态毒理学报, 2018, 13(3):241-252 Sun C H, Liu B X, Lu H F, et al. Luminescent inhibition test of luminous bacteria with the simulated solution of main components of PM2.5[J]. Asian Journal of Ecotoxicology, 2018, 13(3):241-252(in Chinese)
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