3种氟喹诺酮联合暴露对铜锈环棱螺的急性致死效应
Acute Lethal Effects of Combined Exposure to 3 Fluoroquinolones on Bellamya aeruginosa
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摘要: 氟喹诺酮类抗生素(fluoroquinolones, FQs)的大量使用导致其不可避免地进入水环境中，并对水生生物产生毒性作用。当前关于FQs生态毒理的研究大多是基于单独的某种FQs，而关于不同FQs间联合作用的研究较少。本文以铜锈环棱螺(Bellamya aeruginosa)幼体为受试生物，首先分别研究了环丙沙星(ciprofloxacin, CIP)、诺氟沙星(norfloxacin, NOR)和左氧氟沙星(levofloxacin, LEVO)3种FQs的急性致死效应，随后考察了3种FQs的联合毒性作用。结果表明，单一FQ暴露下随着暴露浓度升高和时间的延长，死亡率均呈现上升趋势，3种FQs对铜锈环棱螺的急性毒性作用依次为NOR(LC50: 59.212 mg·L-1)>CIP(LC50: 114.255 mg·L-1)>LEVO(LC50: 123.706 mg·L-1)。铜锈环棱螺对FQs暴露较为敏感，在FQs生态风险评价中有较大应用潜力。联合暴露结果表明，二元系统及三元系统联合毒性作用有所区别，与FQs种类、浓度和暴露时间长短有关，但最终都趋向于协同作用。本研究结果指示虽然FQs单一暴露毒性较弱，但不同FQs的联合作用导致毒性有所增强，应当予以重视并开展深入研究。Abstract: The massive use of fluoroquinolones (FQs) has led to their inevitable introduction into the aquatic environment and their toxic effects on aquatic organisms. Most of the current research on the ecotoxicology of FQs is based on individual FQs, and there are fewer studies on the combined effects of different FQs. In this study, the acute lethal effects of three FQs, ciprofloxacin (CIP), norfloxacin (NOR), and levofloxacin (LEVO), were investigated separately using Bellamya aeruginosa larvae as test organisms. And the combined toxic effects of the three FQs were investigated. The results showed that the mortality enhanced with the increased of exposure concentration and time. The acute lethal effect of the three FQs on Bellamya aeruginosa were in the order of NOR (LC50: 59.212 mg·L-1)>CIP (LC50: 114.255 mg·L-1)>LEVO (LC50: 123.706 mg·L-1). Bellamya aeruginosa is sensitive to FQs exposure and has great application potential in FQs ecological risk assessment. The combined exposure results showed that the joint toxicity effects of binary and ternary system differed and were related to the type, concentration and duration of exposure to FQs, but eventually tended to be synergistic. The results of this study indicate that although the toxicity of a single FQs is relatively weak, but the combined action of different FQs resulted in enhanced toxicity, which should be paid more attention to and carried out further research.
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Key words:
- fluoroquinolones /
- Bellamya aeruginosa /
- acute lethal effect /
- combined toxicity
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