环境污染物诱导细胞膜损伤的研究进展
Research Progress on Cell Membrane Damage Induced by Environmental Pollutants
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摘要: 细胞膜是保护细胞免受外源性污染物破坏的屏障，在细胞与外界进行物质运输、能量转换、信息传递等活动中具有不可替代的作用。当前针对环境污染物诱导细胞膜损伤的研究相对零散，未成体系。本文聚焦近5年国内外环境污染物诱导细胞膜损伤的相关研究并对其进行归纳总结，主要从脂质双分子层、膜蛋白、细胞骨架以及膜微结构域4个方面分析了环境污染物对细胞膜的损伤影响。从脂质双分子层结构变化、脂质过氧化以及膜通透性和完整性等方面描述了环境污染物对脂质双分子层的损伤；从膜蛋白活性、蛋白表达等方面阐述了环境污染物对细胞膜蛋白的损伤；从微管、微丝和中间纤维的变化归纳了环境污染物对细胞骨架的损伤；从脂筏和小窝的变化概括了环境污染物对细胞膜微结构域的损伤。最后，对环境污染物诱导细胞膜损伤的研究现状进行了思考，并对未来研究趋势进行了展望。Abstract: The cell membrane is the barrier that can protect cells from external pollutants, and plays an important role in material transportation, energy conversion, information transmission, and other activities between cell and its outside. At present, the researches on cell membrane damage induced by environmental pollutants are relatively scattered and not systematic. In this paper, we summarized the relevant researches in the past five years and analyzed the effects of environmental pollutants on cell membrane damage from four aspects:lipid bilayers, membrane proteins, cytoskeletons and membrane microdomains. Firstly, the damage of environmental pollutants to lipid bilayers was elaborated from structural changes of lipid bilayers, lipid peroxidation, as well as permeability and integrity of lipid bilayers. Secondly, From the membrane protein activity and protein expression, the damage of environmental pollutants to membrane protein was described. Subsequently, the damage of environmental pollutants to cytoskeletons was elucidated from changes of microtubules, microfilaments and intermediate fibers. Finally, the damage of environmental pollutants to membrane microdomains was summarized from changes in lipid rafts and caveolae. In addition, we considered the current research status and prospected future research trends of cell membrane damage induced by environmental pollutants at the end of this review.
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陆贻通,朱江,余佳丽.环境污染物的分子毒理机制研究进展[J].环境污染与防治,2004,26(3):185-188 ,242-243Lu Y T,Zhu J,Yu J L.Study progress on molecular toxicity mechanism of the environmental pollutant[J].Environmental Pollution&Control,2004,26(3):185-188,242-243(in Chinese)
董芳,李芳芳,祁晓霞,等.环境毒理学研究进展[J].生态毒理学报,2011,6(1):9-17 Dong F,Li F F,Qi X X,et al.Advances in environmentaltoxicology researches[J].Asian Journal of Ecotoxicology,2011,6(1):9-17(in Chinese)
Yang S,Chen Z Z,Cheng Y P,et al.Environmental toxicology wars:Organ-on-a-chip for assessing the toxicity of environmental pollutants[J].Environmental Pollution,2021,268(Pt B):115861 孟紫强.关于生态毒理学与环境毒理学几个基本概念的见解[J].生态毒理学报,2006,1(2):97-104 Meng Z Q.Discussion on some essential concepts of ecotoxicology and environmental toxicology[J].Asian Journal of Ecotoxicology,2006,1(2):97-104(in Chinese)
Shahid M,Manoharadas S,Altaf M,et al.Organochlorinepesticides negatively influenced the cellular growth,morphostructure,cell viability,and biofilm-formation andphosphate-solubilization activities of Enterobacter cloacaestrain EAM 35[J].ACS Omega,2021,6(8):5548-5559 Borisova T.Nervous system injury in response to contact with environmental,engineered and planetary micro-andnano-sized particles[J].Frontiers in Physiology,2018,9:728 Shen M C,Zhang Y X,Zhu Y,et al.Recent advances intoxicological research of nanoplastics in the environment:A review[J].Environmental Pollution,2019,252(Pt A):511-521 Zong L,Xing J P,Liu S,et al.Cell metabolomics revealsthe neurotoxicity mechanism of cadmium in PC12 cells[J].Ecotoxicology and Environmental Safety,2018,147:26-33 Filice F P,Li M S M,Wong J M,et al.The effects of long duration chronic exposure to hexavalentchromium onsingle live cells interrogated by scanning electrochemicalmicroscopy[J].Journal of Inorganic Biochemistry,2018,182:222-229 荆晶,于淼,汝少国.环境污染物对精子损伤机制的研究进展[J].生态毒理学报,2019,14(6):11-22 Jing J,Yu M,Ru S G.Research progress on the mechanisms of sperm damage caused by environmental pollutants[J].Asian Journal of Ecotoxicology,2019,14(6):11-22(in Chinese)
李喜艳,王加启,卜登攀,等.多不饱和脂肪酸对细胞膜功能影响的研究进展[J].生物技术通报,2009,25(12):22-26 Li X Y,Wang J Q,Bu D P,et al.Advanced research of effect of polyunsaturated fatty acids on cell membranefunction[J].Biotechnology Bulletin,2009,25(12):22-26(in Chinese)
Zhang X,Zhang J,Wu Y,et al.Dynamic recovery after acute single fine particulate matter exposure in male mice:Effect on lipid deregulation and cardiovascular alterations[J].Journal of Hazardous Materials,2021,414:125504 Shettihalli A K,Palanirajan S K,Gummadi S N.Are cysteine residues of human phospholipid scramblase 1 essential for Pb2+ and Hg2+ binding-induced scrambling of phospholipids?[J].European Biophysics Journal:EBJ,2021,50(5):745-757 Palanirajan S K,Gummadi S N.Heavy-metals-mediatedphospholipids scrambling by human phospholipid scramblase 3:A probable role in mitochondrial apoptosis[J].Chemical Research in Toxicology,2020,33(2):553-564 Zhang B,Pan N,Fan X Y,et al.Real-time effects of Cd (Ⅱ) on the cellular membrane permeability[J].Analyst,2021,146(19):5973-5979 张标.扫描电化学显微镜技术研究重金属镉对细胞膜通透性的影响[D].北京:北京工业大学,2021:21-28Zhang B.Scanning electrochemical microscopy to studythe effect of heavy metal cadmium on cell membrane permeability[D].Beijing:Beijing University of Technology,2021:21 -28(in Chinese)
Dai J W,Sun C X,Yao Z,et al.Exposure to concentratedambient fine particulate matter disrupts vascular endothelial cell barrier function via the IL-6/HIF-1α signalingpathway[J].FEBS Open Bio,2016,6(7):720-728 Wang L J,Bao S Y,Liu X L,et al.Low-dose exposure toblack carbon significantly increase lung injury of cadmium by promoting cellular apoptosis[J].Ecotoxicologyand Environmental Safety,2021,224:112703 Liu Y Q,Pang X Y,Song J R,et al.Exploring the membrane toxicity of decabromodiphenyl ethane (DBDPE):Based on cell membranes and lipid membranes model[J].Chemosphere,2019,216:524-532 Jarosiewicz M,Duchnowicz P,Jarosiewicz P,et al.An invitro comparative study of the effects of tetrabromobisphenol A and tetrabromobisphenol S on human erythrocyte membranes-changes in ATP level,perturbations inmembrane fluidity,alterations in conformational state anddamage to proteins[J].International Journal of MolecularSciences,2021,22(17):9443 Kim J Y,Lee S Y,Jung S H,et al.Protective effect of Lactobacillus casei HY2782 against particulate matter toxicity in human intestinal CCD-18Co cells and Caenorhabditis elegans[J].Biotechnology Letters,2020,42(4):519-528 袁圣武,黄超,季晓亚,等.环境污染物导致氧化应激的关键信号通路及其检测方法[J].生态毒理学报,2017,12(1):25-37 Yuan S W,Huang C,Ji X Y,et al.Main signaling pathways and detection methods of oxidative stress caused byenvironmental pollutants[J].Asian Journal of Ecotoxicology,2017,12(1):25-37(in Chinese)
Matias A C,Villa dos Santos N,Chelegão R,et al.Cu (GlyGlyHis) effects on MCF7 cells:Copper uptake,reactive oxygen species generation and membrane topographychanges[J].Journal of Inorganic Biochemistry,2012,116:172-179 Ahmad S,Tufail N,Parveen N,et al.Attenuation of Hg (Ⅱ)-induced cellular and DNA damage in humanblood cells by uric acid[J].Biochimie et Biologie Cellulaire,2022,100(1):45-58 Annabi E,Ben Salem I,Abid-Essefi S.Acetamiprid,aneonicotinoid insecticide,induced cytotoxicity and genotoxicity in PC12 cells[J].Toxicology Mechanisms andMethods,2019,29(8):580-586 Sun C Y,Zhang W C,Ding R R,et al.Mechanism of low concentrations of polystyrene microplastics influence thecytotoxicity of Ag ions to Escherichia coli[J].Chemosphere,2020,253:126705 Wang Q Q,Bai J L,Ning B A,et al.Effects of bisphenolA and nanoscale and microscale polystyrene plastic exposure on particle uptake and toxicity in human Caco-2 cells[J].Chemosphere,2020,254:126788 Wang T C,Ma M M,Chen C,et al.Three widely usedpesticides and their mixtures induced cytotoxicity and apoptosis through the ROS-related caspase pathway inHepG2 cells[J].Food and Chemical Toxicology:An International Journal Published for the British Industrial Biological Research Association,2021,152:112162 Zhang Y,Chen H,Fan Y Y,et al.Cytotoxic effects of bio-pesticide spinosad on human lung A549 cells[J].Chemosphere,2019,230:182-189 Zhang Y,Hu B,Qian X L,et al.Transcriptomics-based analysis of co-exposure of cadmium (Cd) and 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) indicates mitochondrial dysfunction induces NLRP3 inflammasome and inflammatory cell death in renal tubular epithelial cells[J].Ecotoxicology and Environmental Safety,2022,241:113790 李剑勇,满亚辉,陈倩,等.膜蛋白的表达:在无细胞体系中实现功能性表达、折叠和组装[J].生物化学与生物物理进展,2018,45(4):389-400 Li J Y,Man Y H,Chen Q,et al.Membrane protein expression:Functional expression,folding and assemblywithin cell free system[J].Progress in Biochemistry andBiophysics,2018,45(4):389-400(in Chinese)
Zeng L,Zhou J Z,Zhang Y W,et al.Differential expression profiles and potential intergenerational functions of tRNA-derived small RNAs in mice after cadmium exposure[J].Frontiers in Cell and Developmental Biology,2021,9:791784 Sule K,Umbsaar J,Prenner E J.Mechanisms of Co,Ni,and Mn toxicity:From exposure and homeostasis to theirinteractions with and impact on lipids and biomembranes[J].Biochimica et Biophysica Acta Biomembranes,2020,1862(8):183250 刘艳青,赵永芳.ABC转运蛋白结构与转运机制的研究进展[J].生命科学,2017,29(3):223-229 Liu Y Q,Zhao Y F.Structure and mechanism of ABCtransporter[J].Chinese Bulletin of Life Sciences,2017,29(3):223-229(in Chinese)
Wu B,Wu X M,Liu S,et al.Size-dependent effects of polystyrene microplastics on cytotoxicity and efflux pumpinhibition in human Caco-2 cells[J].Chemosphere,2019,221:333-341 Yu Y Y,Yin H,Peng H,et al.Proteomic mechanism of decabromodiphenyl ether (BDE-209) biodegradation byMicrobacterium Y2 and its potential in remediation of BDE-209 contaminated water-sediment system[J].Journal of Hazardous Materials,2020,387:121708 Zhou C N,Li X Y.Cytotoxicity of chlorpyrifos to humanliver hepatocellular carcinoma cells:Effects on mitochondrial membrane potential and intracellular free Ca2+[J].Toxin Reviews,2018,37(4):259-268 Ajilore B S,Alli A A,Oluwadairo T O.Effects of magnesium chloride on in vitro cholinesterase and ATPase poisoning by organophosphate (chlorpyrifos)[J].Pharmacology Research&Perspectives,2018,6(3):e00401 Pullarkat P,Fernandez P,Ott A.Rheological properties of the eukaryotic cell cytoskeleton[J].Physics Reports,2007,449(1/3):29-53 MacTaggart B,Kashina A.Posttranslational modifications of the cytoskeleton[J].Cytoskeleton,2021,78(4):142-173 Karki P,Meliton A,Sitikov A,et al.Microtubule destabilization caused by particulate matter contributes to lungendothelial barrier dysfunction and inflammation[J].Cellular Signalling,2019,53:246-255 Pizzaia D,Nogueira M L,Mondin M,et al.Cadmiumtoxicity and its relationship with disturbances in the cytoskeleton,cell cycle and chromosome stability[J].Ecotoxicology,2019,28(9):1046-1055 Ranieri M,Di Mise A,Difonzo G,et al.Green olive leafextract (OLE) provides cytoprotection in renal cells exposed to low doses of cadmium[J].PLoS One,2019,14(3):e0214159 Piscopo M,Notariale R,Tortora F,et al.Novel insightsinto mercury effects on hemoglobin and membrane proteins in human erythrocytes[J].Molecules,2020,25(14):3278 Parisi M G,Pirrera J,la Corte C,et al.Effects of organicmercury on Mytilus galloprovincialis hemocyte functionand morphology[J].Journal of Comparative PhysiologyB,Biochemical,Systemic,and Environmental Physiology,2021,191(1):143-158 Hamdi H,Abid-Essefi S,Eyer J.Neuroprotective effects of myricetin on epoxiconazole-induced toxicity in F98cells[J].Free Radical Biology&Medicine,2021,164:154-163 Hamdi H,Abid-Essefi S,Eyer J.Cytotoxic and genotoxiceffects of epoxiconazole on F98 glioma cells[J].Chemosphere,2019,229:314-323 Hamdi H,Rjiba-Touati K,Ayed-Boussema I,et al.Epoxiconazole caused oxidative stress related DNA damage andapoptosis in PC12 rat pheochromocytoma[J].Neurotoxicology,2022,89:184-190 褚克平.50Hz工频磁场对成纤维细胞微丝骨架装配的影响[D].上海:华东师范大学,2007:2-5Chu K P.Effect of 50Hz power frequency magnetic fieldon microfilament cytoskeleton assembly of fibroblasts[D].Shanghai:East China Normal University,2007 :2-5(in Chinese)
Lu J,Tang M M,Liu Y,et al.Comparative proteomics of chromium-transformed beas-2B cells by 2D-DIGE andMALDI-TOF/TOF MS[J].Biological Trace Element Research,2018,185(1):78-88 Xu D,Liu T,Lin L M,et al.Exposure to endosulfan increases endothelial permeability by transcellular and paracellular pathways in relation to cardiovascular diseases[J].Environmental Pollution,2017,223:111-119 Shi X L,Zha J M,Wen B,et al.Diastereoisomer-specificneurotoxicity of hexabromocyclododecane in human SHSY5Y neuroblastoma cells[J].The Science of the TotalEnvironment,2019,686:893-902 Shi X L,Wen B,Huang H L,et al.Cytotoxicity of hexabromocyclododecane,1,2-dibromo-4-(1,2-dibromoethyl) cyclohexane and 1,2,5,6-tetrabromocyclooctane inhuman SH-SY5Y neuroblastoma cells[J].The Science of the Total Environment,2020,739:139650 Majkova Z,Toborek M,Hennig B.The role of caveolaein endothelial cell dysfunction with a focus on nutritionand environmental toxicants[J].Journal of Cellular andMolecular Medicine,2010,14(10):2359-2370 陈岚,许彩民,袁建刚,等.脂筏的结构与功能[J].生物化学与生物物理进展,2003,30(1):54-59 Chen L,Xu C M,Yuan J G,et al.Structure and function of lipid rafts[J].Progress in Biochemistry and Biophysics,2003,30(1):54-59(in Chinese)
Cacciottolo M,Morgan T E,Saffari A A,et al.Traffic-related air pollutants (TRAP-PM) promote neuronal amyloidogenesis through oxidative damage to lipid rafts[J].FreeRadical Biology&Medicine,2020,147:242-251 Rossi M,Taddei A R,Fasciani I,et al.The cell biology of the thyroid-disrupting mechanism of dichlorodiphenyltrichloroethane (DDT)[J].Journal of Endocrinological Investigation,2018,41(1):67-73 de Gregorio F,Pellegrino M,Picchietti S,et al.The insecticide 1,1,1-trichloro-2,2-bis (p-chlorophenyl) ethane (DDT) alters the membrane raft location of the TSH receptor stably expressed in Chinese hamster ovary cells[J].Toxicology and Applied Pharmacology,2011,253(2):121-129 Rossi M,Scarselli M,Fasciani I,et al.Dichlorodiphenyltrichloroethane (DDT) induced extracellular vesicle formation:A potential role in organochlorine increased risk of Parkinson's disease[J].Acta Neurobiologiae Experimentalis,2017,77(2):113-117 祝妍,屈超,邹伟.小窝蛋白-1在免疫调节中的作用[J].中国生物化学与分子生物学报,2016,32(3):267-273 Zhu Y,Qu C,Zou W.Role of caveolin-1 in regulation of immunity[J].Chinese Journal of Biochemistry and Molecular Biology,2016,32(3):267-273(in Chinese)
Ding Y F,Zhang R Q,Li B Q,et al.Tissue distribution of polystyrene nanoplastics in mice and their entry,transport,and cytotoxicity to GES-1 cells[J].Environmental Pollution,2021,280:116974 Nie J H,Shen Y,Roshdy M,et al.Polystyrene nanoplastics exposure caused defective neural tube morphogenesisthrough caveolae-mediated endocytosis and faulty apoptosis[J].Nanotoxicology,2021,15(7):885-904 Su R J,Jin X T,Li H Q,et al.The mechanisms of PM2.5and its main components penetrate into HUVEC cells andeffects on cell organelles[J].Chemosphere,2020,241:125127 Wei W,Wang Y,Li M,et al.Water-soluble fraction of particulate matter <2.5μm promoted lung epithelia cellsapoptosis by regulating the expression of caveolin-1 andKrüppel-like factor 5[J].Journal of Applied Toxicology,2020,41(3):410-420 Su L,Han L,Ge F,et al.The effect of novel magneticnanoparticles on vascular endothelial cell function in vitroand in vivo[J].Journal of Hazardous Materials,2012,235-236:316-325 Huang Y,He Q.Inhibition of c-Src protects paraquat induced microvascular endothelial injury by modulatingcaveolin-1 phosphorylation and caveolae mediated transcellular permeability[J].Environmental Toxicology andPharmacology,2017,52:62-68 -
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