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不同碳氮比（C/N）条件下驯化微生物的反硝化特性

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孙洪伟, 郭英, 尤永军, 宋相蕊, 孟庆龙, 郝火凡. 不同碳氮比（C/N）条件下驯化微生物的反硝化特性[J]. 环境化学, 2014, 33(5): 770-775. doi: 10.7524/j.issn.0254-6108.2014.05.001
引用本文: 孙洪伟, 郭英, 尤永军, 宋相蕊, 孟庆龙, 郝火凡. 不同碳氮比（C/N）条件下驯化微生物的反硝化特性[J]. 环境化学, 2014, 33(5): 770-775. doi: 10.7524/j.issn.0254-6108.2014.05.001
shu
SUN Hongwei, GUO Ying, YOU Yongjun, SONG Xiangrui, MENG Qinglong, HAO Huofan. Denitrification characteristic of microbial population tamed at different C/N ratios[J]. Environmental Chemistry, 2014, 33(5): 770-775. doi: 10.7524/j.issn.0254-6108.2014.05.001
Citation: SUN Hongwei, GUO Ying, YOU Yongjun, SONG Xiangrui, MENG Qinglong, HAO Huofan. Denitrification characteristic of microbial population tamed at different C/N ratios[J]. Environmental Chemistry, 2014, 33(5): 770-775. doi: 10.7524/j.issn.0254-6108.2014.05.001
shu

不同碳氮比（C/N）条件下驯化微生物的反硝化特性

  • 1.

    兰州交通大学环境与市政工程学院, 兰州, 730070;

  • 2.

    内蒙古自治区水利水电勘测设计院, 呼和浩特, 010020

  • 基金项目:

    国家自然科学基金项目（51168028）；甘肃省省青年基金计划项目（1107RJYA279）；兰州交通大学大学生创新计划项目（201078）资助.

Denitrification characteristic of microbial population tamed at different C/N ratios

  • 1.

    School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou, 730070, China;

  • 2.

    Inner Mongolia Water Resources and Hydropower Survey and Design Institute, Hohhot, 010020, China

  • Fund Project:

  • 摘要: 为了研究不同C/N条件下驯化的微生物的反硝化特性，采用SBR 反应器在4种C/N比（0、5、10、15）条件下对微生物进行驯化.在此基础上，通过批次试验分别考察以NO2--N、NO3--N及NO3--N+NO2--N作为电子受体时，驯化的微生物的反硝化特性.试验结果表明，对于4种C/N比条件下驯化后的微生物，NO2--N和NO3--N两种电子受体均可以被完全还原，但还原速率差异明显.当以NO2--N为电子受体时，4种C/N条件下驯化的微生物的还原速率分别为：0.67、1.10、1.66、0.24 mgN·L-1·min-1.当以NO3--N为电子受体时，随着C/N的增加，4种C/N条件下驯化的微生物的还原速率逐渐增大，分别为：0.31、0.61、0.82 mgN·L-1·min-1和1.01 mgN·L-1·min-1.然而以NO3--N+NO2--N为电子受体时，在C/N为0和5条件下驯化的微生物还原NO2--N的速率高于还原NO3--N的速率，而在C/N为10和15条件下却相反.此外，在初始NOx--N浓度及pH值相同条件下，NO2--N单独还原过程引起的pH增量高于NO3--N单独还原过程，而较低C/N条件下驯化的微生物同步还原NO3--N和NO2--N时，系统内pH的增量较高.
    Abstract: In order to investigate the denitrification characteristic of microbial population tamed with different C/N ratios, microbial population was tamed in sequencing batch reactor (SBRs) with C/N ratios of 0, 5,10 and 15, respectively, and then the denitrification capability of the tamed microbial population was investigated. The effects of different electron acceptors (nitrite, nitrate, nitrite+nitirate) on the reduction capability of tamed microbial population were studied using batch tests. Results showed that both nitrite and nitrate were reduced by the tamed microbial population, but a significant difference in denitrification rate was observed. When nitrite was used as an electron acceptor, the nitrite reduction rates were 0.67, 1.10, 1.66 and 0.24 mgN·L-1·min-1, respectively, for the tamed microbial population with four C/N ratios. The nitrate reduction rates were 0.31, 0.61, 0.82 and 1.01 mgN·L-1·min-1, respectively, using nitrate as an electron acceptor. When nitrite and nitrate co-existed in the system, nitrite reduction rate was higher than those of nitrate for the tamed microbial population with C/N ratios of 0 and 5, while nitrite reduction rate was lower for the tamed microbial population with C/N ratios of 10 and 15. It was also found that pH increased more in nitrite reduction than in nitrate reduction. Significant increase of pH was obtained in co-existing system of nitrite and nitrate for tamed microbial population with C/N ratios of 0 and 5.
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  • 收稿日期:  2013-03-06
孙洪伟, 郭英, 尤永军, 宋相蕊, 孟庆龙, 郝火凡. 不同碳氮比（C/N）条件下驯化微生物的反硝化特性[J]. 环境化学, 2014, 33(5): 770-775. doi: 10.7524/j.issn.0254-6108.2014.05.001
引用本文: 孙洪伟, 郭英, 尤永军, 宋相蕊, 孟庆龙, 郝火凡. 不同碳氮比（C/N）条件下驯化微生物的反硝化特性[J]. 环境化学, 2014, 33(5): 770-775. doi: 10.7524/j.issn.0254-6108.2014.05.001
shu
SUN Hongwei, GUO Ying, YOU Yongjun, SONG Xiangrui, MENG Qinglong, HAO Huofan. Denitrification characteristic of microbial population tamed at different C/N ratios[J]. Environmental Chemistry, 2014, 33(5): 770-775. doi: 10.7524/j.issn.0254-6108.2014.05.001
Citation: SUN Hongwei, GUO Ying, YOU Yongjun, SONG Xiangrui, MENG Qinglong, HAO Huofan. Denitrification characteristic of microbial population tamed at different C/N ratios[J]. Environmental Chemistry, 2014, 33(5): 770-775. doi: 10.7524/j.issn.0254-6108.2014.05.001
shu

不同碳氮比（C/N）条件下驯化微生物的反硝化特性

  • 1.  兰州交通大学环境与市政工程学院, 兰州, 730070;
  • 2.  内蒙古自治区水利水电勘测设计院, 呼和浩特, 010020
  • 收稿日期:  2013-03-06
基金项目:

国家自然科学基金项目（51168028）；甘肃省省青年基金计划项目（1107RJYA279）；兰州交通大学大学生创新计划项目（201078）资助.

摘要: 为了研究不同C/N条件下驯化的微生物的反硝化特性，采用SBR 反应器在4种C/N比（0、5、10、15）条件下对微生物进行驯化.在此基础上，通过批次试验分别考察以NO2--N、NO3--N及NO3--N+NO2--N作为电子受体时，驯化的微生物的反硝化特性.试验结果表明，对于4种C/N比条件下驯化后的微生物，NO2--N和NO3--N两种电子受体均可以被完全还原，但还原速率差异明显.当以NO2--N为电子受体时，4种C/N条件下驯化的微生物的还原速率分别为：0.67、1.10、1.66、0.24 mgN·L-1·min-1.当以NO3--N为电子受体时，随着C/N的增加，4种C/N条件下驯化的微生物的还原速率逐渐增大，分别为：0.31、0.61、0.82 mgN·L-1·min-1和1.01 mgN·L-1·min-1.然而以NO3--N+NO2--N为电子受体时，在C/N为0和5条件下驯化的微生物还原NO2--N的速率高于还原NO3--N的速率，而在C/N为10和15条件下却相反.此外，在初始NOx--N浓度及pH值相同条件下，NO2--N单独还原过程引起的pH增量高于NO3--N单独还原过程，而较低C/N条件下驯化的微生物同步还原NO3--N和NO2--N时，系统内pH的增量较高.

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