高级搜索

四溴双酚A和四氯双酚A对非洲爪蛙蝌蚪的毒性效应

,  ,  , 

downloadPDF

上一篇

下一篇

牛玥, 朱敏, 刘芃岩, 秦占芬. 四溴双酚A和四氯双酚A对非洲爪蛙蝌蚪的毒性效应[J]. 生态毒理学报, 2020, 15(6): 115-122. doi: 10.7524/AJE.1673-5897.20190529001
引用本文: 牛玥, 朱敏, 刘芃岩, 秦占芬. 四溴双酚A和四氯双酚A对非洲爪蛙蝌蚪的毒性效应[J]. 生态毒理学报, 2020, 15(6): 115-122. doi: 10.7524/AJE.1673-5897.20190529001
shu
Niu Yue, Zhu Min, Liu Pengyan, Qin Zhanfen. Toxic Effects of Tetrabromobisphenol A and Tetrachlorobisphenol A on Xenopus laevis Tadpoles[J]. Asian Journal of Ecotoxicology, 2020, 15(6): 115-122. doi: 10.7524/AJE.1673-5897.20190529001
Citation: Niu Yue, Zhu Min, Liu Pengyan, Qin Zhanfen. Toxic Effects of Tetrabromobisphenol A and Tetrachlorobisphenol A on Xenopus laevis Tadpoles[J]. Asian Journal of Ecotoxicology, 2020, 15(6): 115-122. doi: 10.7524/AJE.1673-5897.20190529001
shu

四溴双酚A和四氯双酚A对非洲爪蛙蝌蚪的毒性效应

  • 1. 中国科学院生态环境研究中心, 环境化学与生态毒理学国家重点实验室, 北京 100085;

  • 2. 河北大学化学与环境科学学院, 保定 071002;

  • 3. 中国科学院大学, 北京 100049

    • 作者简介: 牛玥(1995-),女,硕士,研究方向为毒理学,E-mail:296046855@qq.com
    通讯作者: 秦占芬, E-mail: qinzhanfen@rcees.ac.cn
  • 基金项目:

    国家重点研发计划资助项目（2017YFF0211203）；国家自然科学基金面上项目（21876196）

  • 中图分类号: X171.5

Toxic Effects of Tetrabromobisphenol A and Tetrachlorobisphenol A on Xenopus laevis Tadpoles

  • 1. State Key Laboratory of Environmental Chemistry and Ecological Toxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;

  • 2. School of Chemistry and Environmental Sciences, Hebei University, Baoding 071002, China;

  • 3. University of Chinese Academy of Sciences, Beijing 100049, China

    • Corresponding author: Qin Zhanfen, qinzhanfen@rcees.ac.cn
  • Fund Project:

  • 摘要: 四溴双酚A（tetrabromobisphenol A，TBBPA）和四氯双酚A（tetrachlorobisphenol A，TCBPA）作为阻燃剂被大量生产和使用，其毒性效应受到关注。相对鱼类和哺乳类动物，TBBPA和TCBPA对两栖动物的毒性数据还比较缺乏。本文研究了TBBPA和TCBPA对非洲爪蛙蝌蚪的急性毒性，并比较了二者对氧化应激标记基因和凝血相关基因转录水平的影响。结果表明，TBBPA和TCBPA的48 h半致死浓度分别为4.31 mg·L-1和3.99 mg·L-1；在无蝌蚪死亡浓度下，TBBPA和TCBPA均能显著影响蝌蚪体内典型氧化应激标记基因的表达，其中，对谷胱甘肽转移酶（glutathione transferase）gst基因的影响最显著，TBBPA和TCBPA的效应相近；TBBPA和TCBPA对蝌蚪体内热休克蛋白的转录影响不明显；另外发现，TBBPA和TCBPA可导致蝌蚪产生凝血现象，同时凝血相关基因的转录水平被上调。综上可知，TBBPA和TCBPA对蝌蚪的急性毒性都为中毒，氧化应激效应也接近，但不引起热休克蛋白转录的变化。
    Abstract: Tetrabromobisphenol A (TBBPA) and tetrachlorobisphenol A (TCBPA), as flame retardants, are extensively produced and used, and their toxic effects have raised more concerns. Little is known about their toxic effects on amphibians relative to fish and mammals. In this study, we investigated their acute toxicities and effects on oxidative stress markers in Xenopus laevis tadpoles. As a result, 48 h median lethal concentration values of TBBPA and TCBPA were 4.31 mg·L-1 and 3.99 mg·L-1, respectively. At no death concentrations, TBBPA and TCBPA affected transcriptional levels of typical marker genes for oxidative stress, with the most dramatic effect on glutathione transferase gene gst. TBBPA and TCBPA had no significant effects on heat shock protein (HSP) gene expression. Unexpectedly, we found that TBBPA and TCBPA resulted in coagulation in hearts and tails, coupled with the transcriptional up-regulation of coagulation genes. The results show that TBBPA and TCBPA had the medium toxicity to tadpoles, with similar effects on oxidative stress and no effects on HSP gene transcription.
  • 加载中
  • Covaci A, Voorspoels S, Abdallah M A, et al. Analytical and environmental aspects of the flame retardant tetrabromobisphenol-A and its derivatives[J]. Journal of Chromatography A, 2009, 1216(3):346-363
    Liu K, Li J, Yan S J, et al. A review of status of tetrabromobisphenol A (TBBPA) in China[J]. Chemosphere, 2016, 148:8-20
    Birnbaum L S, Staskal D F. Brominated flame retardants:Cause for concern?[J]. Environmental Health Perspectives, 2004, 112(1):9-17
    Kim U J, Oh J E. Tetrabromobisphenol A and hexabromocyclododecane flame retardants in infant-mother paired serum samples, and their relationships with thyroid hormones and environmental factors[J]. Environmental Pollution, 2014, 184:193-200
    Malkoske T, Tang Y L, Xu W Y, et al. A review of the environmental distribution, fate, and control of tetrabromobisphenol A released from sources[J]. The Science of the Total Environment, 2016, 569-570:1608-1617
    Jakobsson K, Thuresson K, Rylander L, et al. Exposure to polybrominated diphenyl ethers and tetrabromobisphenol A among computer technicians[J]. Chemosphere, 2002, 46(5):709-716
    Morris S, Allchin C R, Zegers B N, et al. Distribution and fate of HBCD and TBBPA brominated flame retardants in North Sea estuaries and aquatic food webs[J]. Environmental Science & Technology, 2004, 38(21):5497-5504
    Rodríguez-Gómez R, Jiménez-Díaz I, Zafra-Gómez A, et al. A multiresidue method for the determination of selected endocrine disrupting chemicals in human breast milk based on a simple extraction procedure[J]. Talanta, 2014, 130:561-570
    Chu S G, Haffner G D, Letcher R J. Simultaneous determination of tetrabromobisphenol A, tetrachlorobisphenol A, bisphenol A and other halogenated analogues in sediment and sludge by high performance liquid chromatography-electrospray tandem mass spectrometry[J]. Journal of Chromatography A, 2005, 1097(1-2):25-32
    Liu K, Li J, Yan S J, et al. A review of status of tetrabromobisphenol A (TBBPA) in China[J]. Chemosphere, 2016, 148:8-20
    Yang S W, Wang S R, Liu H L, et al. Tetrabromobisphenol A:Tissue distribution in fish, and seasonal variation in water and sediment of Lake Chaohu, China[J]. Environmental Science and Pollution Research International, 2012, 19(9):4090-4096
    Wang X M, Liu J Y, Liu A F, et al. Preparation and evaluation of mesoporous cellular foams coating of solid-phase microextraction fibers by determination of tetrabromobisphenol A, tetrabromobisphenol S and related compounds[J]. Analytica Chimica Acta, 2012, 753:1-7
    Fan Z L, Hu J Y, An W, et al. Detection and occurrence of chlorinated byproducts of bisphenol A, nonylphenol, and estrogens in drinking water of China:Comparison to the parent compounds[J]. Environmental Science & Technology, 2013, 47(19):10841-10850
    US National Institute of Health (US NIH). National Library of Medicine and Toxicology Data Network, 4,4'-Methylenebis(phenol). (2014-04-28). http://chem.sis.nlm.nih.gov/chemid-plus/ProxyServlet?ObjectHandle=DBMaint&actionHandle=default&nextPage=jsp/chemidlite/Result-Screen.jsp&TXTS-UPERLISTID=0000620928
    Hallgren S, Sinjari T, Håkansson H, et al. Effects of polybrominated diphenyl ethers (PBDEs) and polychlorinated biphenyls (PCBs) on thyroid hormone and vitamin A levels in rats and mice[J]. Archives of Toxicology, 2001, 75(4):200-208
    Chan W K, Chan K M. Disruption of the hypothalamic-pituitary-thyroid axis in zebrafish embryo-larvae following waterborne exposure to BDE-47, TBBPA and BPA[J]. Aquatic Toxicology, 2012, 108:106-111
    巩文静, 朱丽岩, 郝雅, 等. 四溴双酚A对太平洋真宽水蚤的急性毒性及氧化胁迫[J]. 生态毒理学报, 2016, 11(4):232-238

    Gong W J, Zhu L Y, Hao Y, et al. Acute toxicity and oxidative stress of tetrabromobis-phenol A to Eurytemora pacifica[J]. Asian Journal of Ecotoxicology, 2016, 11(4):232-238(in Chinese)

    Hu J, Liang Y, Chen M J, et al. Assessing the toxicity of TBBPA and HBCD by zebrafish embryo toxicity assay and biomarker analysis[J]. Environmental Toxicology, 2009, 24(4):334-342
    Wu S M, Ji G X, Liu J N, et al. TBBPA induces developmental toxicity, oxidative stress, and apoptosis in embryos and zebrafish larvae (Danio rerio)[J]. Environmental Toxicology, 2016, 31(10):1241-1249
    Finkel T, Holbrook N J. Oxidants, oxidative stress and the biology of ageing[J]. Nature, 2000, 408(6809):239-247
    Lou Q Q, Cao S, Xu W, et al. Molecular characterization and mRNA expression of ribosomal protein L8 in Rana nigromaculata during development and under exposure to hormones[J]. Journal of Environmental Sciences, 2014, 26(11):2331-2339
    Festing M F, Altman D G. Guidelines for the design and statistical analysis of experiments using laboratory animals[J]. ILAR Journal, 2002, 43(4):244-258
    Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method[J]. Methods, 2001, 25(4):402-408
    中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会. 危险化学品鱼类急性毒性分级试验方法:GB/T 21281-2007[S]. 北京:中国标准出版社, 2008
    Song M Y, Liang D, Liang Y, et al. Assessing developmental toxicity and estrogenic activity of halogenated bisphenol A on zebrafish (Danio rerio)[J]. Chemosphere, 2014, 112:275-281
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  1970
  • HTML全文浏览数:  1970
  • PDF下载数:  70
  • 施引文献:  0
出版历程
  • 收稿日期:  2019-05-29

四溴双酚A和四氯双酚A对非洲爪蛙蝌蚪的毒性效应

    通讯作者: 秦占芬, E-mail: qinzhanfen@rcees.ac.cn
    作者简介: 牛玥(1995-),女,硕士,研究方向为毒理学,E-mail:296046855@qq.com
  • 1. 中国科学院生态环境研究中心, 环境化学与生态毒理学国家重点实验室, 北京 100085;
  • 2. 河北大学化学与环境科学学院, 保定 071002;
  • 3. 中国科学院大学, 北京 100049
  • 收稿日期:  2019-05-29
基金项目:

国家重点研发计划资助项目（2017YFF0211203）；国家自然科学基金面上项目（21876196）

摘要: 四溴双酚A（tetrabromobisphenol A，TBBPA）和四氯双酚A（tetrachlorobisphenol A，TCBPA）作为阻燃剂被大量生产和使用，其毒性效应受到关注。相对鱼类和哺乳类动物，TBBPA和TCBPA对两栖动物的毒性数据还比较缺乏。本文研究了TBBPA和TCBPA对非洲爪蛙蝌蚪的急性毒性，并比较了二者对氧化应激标记基因和凝血相关基因转录水平的影响。结果表明，TBBPA和TCBPA的48 h半致死浓度分别为4.31 mg·L-1和3.99 mg·L-1；在无蝌蚪死亡浓度下，TBBPA和TCBPA均能显著影响蝌蚪体内典型氧化应激标记基因的表达，其中，对谷胱甘肽转移酶（glutathione transferase）gst基因的影响最显著，TBBPA和TCBPA的效应相近；TBBPA和TCBPA对蝌蚪体内热休克蛋白的转录影响不明显；另外发现，TBBPA和TCBPA可导致蝌蚪产生凝血现象，同时凝血相关基因的转录水平被上调。综上可知，TBBPA和TCBPA对蝌蚪的急性毒性都为中毒，氧化应激效应也接近，但不引起热休克蛋白转录的变化。

English Abstract

    全文HTML

参考文献 (25)

目录

/

返回文章
返回

Copyright © 2019 中国科学院生态环境研究中心

京ICP备05002858号-9