摘要:
铜锈环棱螺作为一种新兴的潜在生态毒理学模式生物,适宜实验条件是开展其室内毒理学研究的基础。在人为控制条件下,初步探讨了饵料、水深、底质、密度和钙离子浓度养殖条件对铜锈环棱螺成体生长的影响,并进一步对麻醉条件进行了筛选(MgCl2·2H2O、乙醇、丁香酚、盐酸普鲁卡因和MS-222)。结果表明:当投喂冰鲜小球藻、密度6个·L-1水体积、水深10~15cm、泥土底质并且水中钙离子浓度30 mg·L-1时,铜锈环棱螺的体重增长最为明显。麻醉效果表明,MgCl2·2H2O对成螺的麻醉时间短并且伤害小,可作为实验用麻醉剂首选。以上研究结果为发展以铜锈环棱螺为模式生物开展环境(生态)毒理学研究奠定了基础。
Abstract:
Bellamya aeruginosa could be used as a potential ecotoxicological model organism; however, the first requirement for future research is to optimize laboratory conditions. The present study optimized food, water depth, bottom materials, density, and calcium ion concentration for Bellamya aeruginosa cultured under artificial conditions using weight gain ratio as the endpoint, and then followed by evaluation of different anesthetics including MgCl2·2H2 O, ethanol, eugenol, procaine hydrochloride and MS-222. Results indicated that growth was optimal with the following conditions:feeding fresh C. vulgaris, culturing at a density of 6 number·L-1, keeping the water depth at 10~15 cm, using fine soil as the bottom material and keeping the calcium ion concentration at 30 mg·L-1. Furthermore, results showed that MgCl2·2H2 O should be the primary choice of anesthetic for this organism. These conditions will result in proper rearing and maintenance of Bellamya aeruginosa for use as a model organism in future.
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