全氟辛烷磺酸(PFOS)和多壁碳纳米管(MWCNTs)复合对斑马鱼外周血红细胞DNA的损伤
DNA Damage in Peripheral Blood Red Cells of Zebrafish Induced by Cotreatment of Perfluorooctane Sulfonate(PFOS) and Multiwall Carbon Nanotubes(MWCNTs)
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摘要: 为了评价全氟辛烷磺酸(PFOS)和多壁碳纳米管(MWCNTs)对水环境及鱼类的影响,以斑马鱼为模式生物,研究了PFOS和MWCNTs复合对斑马鱼外周血红细胞的DNA损伤。将成年斑马鱼暴露于PFOS(0.2、0.4、0.8、1.6 mg·L-1)、MWCNTs(50 mg·L-1)、PFOS+MWCNTs(0.2+50、0.4+50、0.8+50、1.6+50 mg·L-1)和对照溶液中30 d后,断尾取血进行微核试验和彗星试验。结果表明:PFOS和MWCNTs均可造成斑马鱼外周血红细胞的DNA损伤。1.6 mg·L-1PFOS处理组的微核率、Olive尾矩及尾长分别为(36.3±0.25)‰、(87.91±14.90)μm和(250.49±34.71)μm。PFOS与MWCNTs复合后,斑马鱼外周血红细胞的DNA损伤效应明显降低。复合处理组斑马鱼外周血红细胞的微核率、Olive尾矩及尾长均低于PFOS单独处理相。1.6 mg·L-1复合处理组的微核率、Olive尾矩及尾长比PFOS单独处理组分别降低了24.7%、68.9%、52.4%。因此,在实验浓度范围内,MWCNTs可以降低PFOS对斑马鱼外周血红细胞的DNA损伤。Abstract: In order to assess the effect of perfluorooctane sulfonate (PFOS) and multiwall carbon nanotubes (MWCNTs) on aquatic environment and fish, zebrafish was chosen as a model animal, and then the DNA damage in peripheral blood red cells of zebrafish after exposing to PFOS and MWCNTs was studied. The adult zebrafish were exposed to PFOS (0.2、0.4、0.8、1.6 mg·L-1)、MWCNTs (50 mg·L-1)、PFOS + MWCNTs (0.2 +50、0.4 +50、0.8 +50、1.6 +50 mg·L-1) and the control for 30 days. Then the peripheral blood red cells of zebrafish were tested for micronucleus and comet assay. The results showed that both PFOS and MWCNTs induced DNA damage in peripheral blood red cells of zebrafish. Micronucleus frequency (MNF)、Olive tail moment (OTM) and tail length (TL) were (36.3 ±0.25) ‰、(87.91 ±14.90) μm and (250.49 ±34.71) μm in 1.6 mg·L-1 PFOS treatment group, respectively. DNA damage was reduced in peripheral blood red cells of zebrafish after co-exposure of PFOS and MWCNTs. The MNF, OTM and TL in PFOS co-exposure groups showed a significant reduction compared with that in PFOS single exposure. The MNF, OTM and TL in 1.6 mg·L-1 PFOS co-exposure were reduced 24.7%、68.9% and 52.4%, compared with that in 1.6 mg·L-1 PFOS, respectively. So MWCNTs could reduce DNA damage of PFOS in peripheral blood red cells of zebrafish at the experimental concentrations.
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