5氟尿嘧啶对蛋白核小球藻和羊角月芽藻生长及叶绿素含量的影响

李威; 恽烨; 是怡芸; 张银龙

doi:10.7524/AJE.1673-5897.20150720001

1. 南京林业大学江苏省南方现代林业协同创新中心, 南京 210037;

2. 南京林业大学生物与环境学院, 南京 210037;

3. 镇江市计量检定测试中心, 镇江 212132

作者简介: 李威(1982-),女,博士,研究方向为污染生态化学,Email:uwliwei@163.com.

基金项目:

江苏省高校自然科学研究面上项目(15KJB610006)；江苏高校优势学科建设工程资助项目(PAPD)；南京林业大学高层次人才基金项目(163010675)

中图分类号: X171.5

Effects of 5-Fluorouracil on the Growth and Chlorophyll Content of Chlorella pyrenoidosa and Selenastrum capricornutum

1. Collaborative Innovation Center of Sustainable Forestry in Southern China of Jiangsu Province, Nanjing Forestry University, Nanjing 210037, China;

2. College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China;

3. Zhenjiang Metrological Verification and Testing Center, Zhenjiang 212132, China

Fund Project:

摘要: 近年来，抗癌药的环境污染特征及其生态风险引起了广泛关注。为获取典型抗癌药5氟尿嘧啶的基础生态毒性数据，以蛋白核小球藻(Chlorella pyrenoidosa)和羊角月芽藻(Selenastrum capricornutum)为受试生物，考察了5氟尿嘧啶对2种绿藻的生长和叶绿素含量的影响。结果表明，5氟尿嘧啶对蛋白核小球藻和羊角月芽藻的生长具有抑制作用，随着暴露浓度升高，细胞生长抑制率增强。5氟尿嘧啶对2种绿藻的96 h半数抑制浓度(EC₅₀)分别为450.36 mg·L^-1和692.30 mg·L^-1，属于低毒性物质。暴露96 h后，低浓度5氟尿嘧啶(<32 mg·L^-1)对蛋白核小球藻和羊角月芽藻叶绿素含量有一定的促进作用，高浓度5氟尿嘧啶(32~500 mg·L^-1)则抑制了2种绿藻的叶绿素含量，且两者具有明显的负相关关系。和叶绿素b相比，叶绿素a对5氟尿嘧啶胁迫更为敏感。

Abstract: The environmental occurrence and ecotoxicological risk of anticancer drugs (also called antineoplastic drugs) have attracted wide attentions. To obtain the basic ecotoxicology data of 5-fluorouracil, a typical anticancer drug, Chlorella pyrenoidosa and Selenastrum capricornutum were employed as target organisms, and effect of 5-fluorouracil on the growth and chlorophyll content of these two algae were examined. The results showed that the growth of C. pyrenoidosa and S. capricornutum were inhibited by 5-fluorouracil. The percentage of growth inhibition was enhanced with the increase of 5-fluorouracil concentration. The 96 h median effective concentration (EC₅₀) of 5-fluorouracil to C. pyrenoidosa and S. capricornutum was 450.36 mg·L^-1 and 692.30 mg·L^-1, respectively. 5-fluorouracil was low toxic to the two algae. After exposure to 5-fluorouracil for 96 h, higher chlorophyll content of C. pyrenoidosa and S. capricornutum was observed under low concentration of 5-fluorouracil (<32 mg·L^-1), while high concentration of 5-fluorouracil (32-500 mg·L^-1) inhibited chlorophyll a and b content of C. pyrenoidosa and S. capricornutum with a negative correlation. Chlorophyll a is more sensitive to 5-fluorouracil.

Key words:

5氟尿嘧啶对蛋白核小球藻和羊角月芽藻生长及叶绿素含量的影响

作者简介: 李威(1982-),女,博士,研究方向为污染生态化学,Email:uwliwei@163.com.
1. 南京林业大学江苏省南方现代林业协同创新中心, 南京 210037;

2. 南京林业大学生物与环境学院, 南京 210037;

3. 镇江市计量检定测试中心, 镇江 212132

收稿日期: 2015-07-20

基金项目:

江苏省高校自然科学研究面上项目(15KJB610006)；江苏高校优势学科建设工程资助项目(PAPD)；南京林业大学高层次人才基金项目(163010675)

关键词:

Effects of 5-Fluorouracil on the Growth and Chlorophyll Content of Chlorella pyrenoidosa and Selenastrum capricornutum

1. Collaborative Innovation Center of Sustainable Forestry in Southern China of Jiangsu Province, Nanjing Forestry University, Nanjing 210037, China;

2. College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China;

3. Zhenjiang Metrological Verification and Testing Center, Zhenjiang 212132, China

Received Date: 2015-07-20

Fund Project:

Keywords:

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