多环芳烃类污染物对斑马鱼胁迫效应的研究进展
Advances in Research on Stress Effects of Polycyclic Aromatic Hydrocarbons on Zebrafish
-
摘要: 伴随着现代工业的飞速发展，不同环境介质中污染物的种类、污染范围和污染强度也在不断改变。作为一种强生物损伤性物质，多环芳烃类污染物备受关注。鉴于生态环境中多环芳烃的污染问题日益凸显，对其毒性效应机制研究的概括和总结尤为重要。因此笔者综述了多环芳烃类污染物对斑马鱼不同生物结构层次的毒性效应研究进展，总结并比较了不同多环芳烃类污染物的毒性效应机制和毒性强度。同时就目前斑马鱼在水环境污染评价、水质综合毒性测定方面的应用进行了概述和展望。研究多环芳烃类污染物对斑马鱼的急性毒性作用和富集作用及其分子机制将对开展水环境中突发有机污染的早期预警及水环境安全评估、人体健康评估具有重要意义。
-
关键词:
- 多环芳烃(PAHs) /
- 斑马鱼 /
- 致毒机制 /
- 水环境安全评估
Abstract: With the rapid development of modern industry, the types, scope, and intensity of pollutants in different environmental media are also constantly changing. Polycyclic aromatic hydrocarbons (PAHs) contained in pollutants have attracted much attention as a type of strong biologically damaging substance. PAHs mainly exist in air and water, easily entering into the human body. Because of their fat-soluble properties, they are difficult to be eliminated after entering into the human body, which causes great harm to human body. A certain amount of PAHs usually exists in natural environment such as atmospheric environment, water environment, and soil environment. The existence of PAHs is more obvious in some areas with dense populations, developed industries, and frequent traffic. Since the pollution of PAHs in the ecological environment is becoming increasingly prominent, the research on the mechanism of their toxicity is particularly important. In recent years, with the frequent appearance of health problems caused by PAHs, the toxic mechanism of PAHs has attracted more and more attention. The morphology of zebrafish during its growth and development is quite similar to that of human, and the signal pathways and genes involved at the cellular and molecular levels generally have a high degree of homology. With the deepening and specificity of water pollution research, the contribution of zebrafish, as a model organism, is increasing, and its applications are becoming more and more extensive as well. This paper reviews the research progress of the toxic effects of PAHs on zebrafish at different biological structure levels, summarizes the acute toxic effects of different PAHs on adult zebrafish and their embryos, so as to compare the mechanisms of toxic effects and toxic intensities of different PAHs pollutants. In this paper, we will focus on the research progress in the areas of behavior, physiology, biochemistry, and molecular mechanisms of zebrafish exposed to PAHs pollutants. At the same time, the application of zebrafish in the evaluation of water environment pollution and the determination of comprehensive water quality toxicity is summarized and prospected. PAHs in the water environment will enter aquatic organisms through bioaccumulation and continue to accumulate, thus changing the biological effects of organisms and causing harm to organisms. The study of the acute toxicity and enrichment of PAHs on zebrafish as well as its molecular mechanism will be of great significance to the early warning of sudden organic pollution in water environment, and the assessment of water environment safety and human health. -
-
吴明红, 陈镠璐, 陈祖怡, 等. 多环芳烃在上海近郊大气颗粒物(PM2.5和PM10)中的污染特征、来源及其健康风险评估[J]. 上海大学学报: 自然科学版, 2014, 20(4): 521-530 Wu M H, Chen L L, Chen Z Y, et al. Polycyclic aromatic hydrocarbons (PAHs) in atmospheric particulate matter (PM2.5 and PM10) from a suburban area in Shanghai: Levels, sources and risk assessment [J]. Journal of Shanghai University: Natural Science Edition, 2014, 20(4): 521-530 (in Chinese)
Vascotto S G, Beckham Y, Kelly G M. The zebrafish’s swim to fame as an experimental model in biology [J]. Biochemistry and Cell Biology, 1997, 75(5): 479-485 赵文昌, 程金平, 谢海, 等. 环境中多环芳烃(PAHs)的来源与监测分析方法[J]. 环境科学与技术, 2006, 29(3): 105-107 , 121 Zhao W C, Cheng J P, Xie H, et al. PAHs: Sources, pathway and their monitoring and analysis [J]. Environmental Science & Technology, 2006, 29(3): 105-107, 121 (in Chinese)
黄东龙, 周勤. 水体突发性重金属污染胁迫下斑马鱼的行为反应分析[J]. 环境监测管理与技术, 2011, 23(4): 27-31 Huang D L, Zhou Q. Behavior response analysis of zebrafish Danio rerio under sudden heavy metal stress [J]. The Administration and Technique of Environmental Monitoring, 2011, 23(4): 27-31 (in Chinese)
Mohanty R, Das S K, Singh N R, et al. Withania somnifera leaf extract ameliorates benzopyrene-induced behavioral and neuromorphological alterations by improving brain antioxidant status in zebrafish (Danio rerio) [J]. Zebrafish, 2016, 13(3): 188-196 Das S K, Aparna S, Patri M. Chronic waterborne exposure to benzopyrene induces locomotor dysfunction and development of neurodegenerative phenotypes in zebrafish [J]. Neuroscience Letters, 2020, 716: 134646 Vignet C, le Menach K, Lyphout L, et al. Chronic dietary exposure to pyrolytic and petrogenic mixtures of PAHs causes physiological disruption in zebrafish: Part Ⅱ: Behavior [J]. Environmental Science and Pollution Research International, 2014, 21(24): 13818-13832 Aparna S, Patri M. Benzopyrene exposure and overcrowding stress impacts anxiety-like behavior and impairs learning and memory in adult zebrafish, Danio rerio [J]. Environmental Toxicology, 2020, 36(3): 352-361 王明亮, 丁永芳, 印鑫, 等. 斑马鱼在毒理学中的应用研究进展[J]. 中国实验动物学报, 2020, 28(3): 390-396 Wang M L, Ding Y F, Yin X, et al. Research progress regarding the application of zebrafish in toxicology [J]. Acta Laboratorium Animalis Scientia Sinica, 2020, 28(3): 390-396 (in Chinese)
Alves R N, Mariz C F Jr, de Paulo D V, et al. Toxicity of effluents from gasoline stations oil-water separators to early life stages of zebrafish Danio rerio [J]. Chemosphere, 2017, 178: 224-230 Perrichon P, le Menach K, Akcha F, et al. Toxicity assessment of water-accommodated fractions from two different oils using a zebrafish (Danio rerio) embryo-larval bioassay with a multilevel approach [J]. Science of the Total Environment, 2016, 568: 952-966 Li X S, Ding G H, Xiong Y J, et al. Toxicity of water-accommodated fractions (WAF), chemically enhanced WAF (CEWAF) of Oman crude oil and dispersant to early-life stages of zebrafish (Danio rerio) [J]. Bulletin of Environmental Contamination and Toxicology, 2018, 101(3): 314-319 蔡吉榛, 罗显桥, 林红英, 等. 湛江近海域沉积物含氯多环芳烃对斑马鱼胚胎的毒性研究[J]. 广东海洋大学学报, 2013, 33(1): 72-76 Cai J Z, Luo X Q, Lin H Y, et al. Toxicity of chlorinated polycyclic aromatic hydrocarbons extracted from Zhanjiang coastal sediment to zebrafish embryo [J]. Journal of Guangdong Ocean University, 2013, 33(1): 72-76 (in Chinese)
Willis A M, Oris J T. Acute photo-induced toxicity and toxicokinetics of single compounds and mixtures of polycyclic aromatic hydrocarbons in zebrafish [J]. Environmental Toxicology and Chemistry, 2014, 33(9): 2028-2037 González-Doncel M, González L, Fernández-Torija C, et al. Toxic effects of an oil spill on fish early life stages may not be exclusively associated to PAHs: Studies with Prestige oil and medaka (Oryzias latipes) [J]. Aquatic Toxicology, 2008, 87(4): 280-288 Diamante G, do Amaral e Silva Müller G, Menjivar-Cervantes N, et al. Developmental toxicity of hydroxylated chrysene metabolites in zebrafish embryos [J]. Aquatic Toxicology, 2017, 189: 77-86 Zhai Y W, Xia X H, Xiong X Y, et al. Role of fluoranthene and pyrene associated with suspended particles in their bioaccumulation by zebrafish (Danio rerio) [J]. Ecotoxicology and Environmental Safety, 2018, 157: 89-94 Xie S L, Zhou A G, Xu N, et al. Benzopyrene induces microbiome dysbiosis and inflammation in the intestinal tracts of western mosquitofish (Gambusia affinis) and zebrafish (Danio rerio) [J]. Fish & Shellfish Immunology, 2020, 105: 24-34 Vignet C, Larcher T, Davail B, et al. Fish reproduction is disrupted upon lifelong exposure to environmental PAHsfractions revealing different modes of action [J]. Toxics, 2016, 4(4): 26 Hicken C E, Linbo T L, Baldwin D H, et al. Sublethal exposure to crude oil during embryonic development alters cardiac morphology and reduces aerobic capacity in adult fish [J]. Proceedings of the National Academy of Sciences of the United States of America, 2011, 108(17): 7086-7090 Wang H T, Li Y Y, Xia X H, et al. Relationship between metabolic enzyme activities and bioaccumulation kinetics of PAHs in zebrafish (Danio rerio) [J]. Journal of Environmental Sciences, 2018, 65: 43-52 Timme-Laragy A R, Cockman C J, Matson C W, et al. Synergistic induction of AHR regulated genes in developmental toxicity from co-exposure to two model PAHs in zebrafish [J]. Aquatic Toxicology, 2007, 85(4): 241-250 Wincent E, Jönsson M E, Bottai M, et al. Aryl hydrocarbon receptor activation and developmental toxicity in zebrafish in response to soil extracts containing unsubstituted and oxygenated PAHs[J]. Environmental Science & Technology, 2015, 49(6): 3869-3877 Kim S, Sohn J H, Ha S Y, et al. Thyroid hormone disruption by water-accommodated fractions of crude oil and sediments affected by the Hebei spirit oil spill in zebrafish and GH3 cells [J]. Environmental Science & Technology, 2016, 50(11): 5972-5980 谭敏卿. 菲和高氯酸钠对斑马鱼的毒性效应[D]. 哈尔滨: 哈尔滨工业大学, 2011: 27-32 Tan M Q. The toxicity of phenanthrene and sodium percolorate to zebrafish [D]. Harbin: Harbin Institute of Technology, 2011: 27 -32 (in Chinese)
Mai Y Z, Peng S Y, Li H Y, et al. Histological, biochemical and transcriptomic analyses reveal liver damage in zebrafish (Danio rerio) exposed to phenanthrene [J]. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 2019, 225: 108582 Ning X, Wang Y, Zhu N, et al. Risk assessment of the lipid metabolism-disrupting effects of nitro-PAHs[J]. Journal of Hazardous Materials, 2020, 396: 122611 Wegermann K, Hyun J, Diehl A M. Molecular mechanisms linking nonalcoholic steatohepatitis to cancer [J]. Clinical Liver Disease, 2021, 17(1): 6-10 宋静文, 靳亚茹, 刘红玲. 典型酚类污染物内分泌干扰效应研究: 对斑马鱼发育及核受体介导基因调控的分子影响[J]. 中国环境科学, 2020, 40(9): 4065-4076 Song J W, Jin Y R, Liu H L. Research on the endocrine disruption effect of typical phenolic pollutants: The embryonic development effects and molecule effects of gene regulation mediated by nuclear receptor onzebrafish [J]. China Environmental Science, 2020, 40(9): 4065-4076 (in Chinese)
Bui A, Xiao R, Perveen Z, et al. Zebrafish embryos sequester and retain petrochemical combustion products: Developmental and transcriptome consequences [J]. Aquatic Toxicology, 2012, 108: 23-32 Shankar P, Geier M C, Truong L, et al. Coupling genome-wide transcriptomics and developmental toxicity profiles in zebrafish to characterize polycyclic aromatic hydrocarbon (PAH) hazard [J]. International Journal of Molecular Sciences, 2019, 20(10): 2570 Garcia G R, Shankar P, Dunham C L, et al. Signaling events downstream of AHR activation that contribute to toxic responses: The functional role of an AHR-dependent long noncoding RNA (slincR) using the zebrafish model [J]. Environmental Health Perspectives, 2018, 126(11): 117002 McIntyre J K, Edmunds R C, Redig M G, et al. Confirmation of stormwater bioretention treatment effectiveness using molecular indicators of cardiovascular toxicity in developing fish [J]. Environmental Science & Technology, 2016, 50(3): 1561-1569 Fang X F, Corrales J, Thornton C, et al. Transcriptomic changes in zebrafish embryos and larvae following benzopyrene exposure [J]. Toxicological Sciences, 2015, 146(2): 395-411 Yun Y, Zhang Y J, Li G K, et al. Embryonic exposure to oxy-polycyclic aromatic hydrocarbon interfere with pancreatic β-cell development in zebrafish via altering DNA methylation and gene expression [J]. Science of the Total Environment, 2019, 660: 1602-1609 Elie M R, Choi J, Nkrumah-Elie Y M, et al. Metabolomic analysis to define and compare the effects of PAHs and oxygenated PAHs in developing zebrafish [J]. Environmental Research, 2015, 140: 502-510 Sogbanmu T O, Nagy E, Phillips D H, et al. Lagos lagoon sediment organic extracts and polycyclic aromatic hydrocarbons induce embryotoxic, teratogenic and genotoxic effects in Danio rerio (zebrafish) embryos [J]. Environmental Science and Pollution Research International, 2016, 23(14): 14489-14501 Fujita K K, Xia Z, Tomy G, et al. 1H NMR based metabolomic profiling of early life stage zebrafish (Danio rerio) exposed to a water-soluble fraction of weathered sediment-bound diluted bitumen [J]. Aquatic Toxicology, 2021, 232: 105766 陈宏姗, 盛连喜, 边红枫. 化合物萘对斑马鱼(Danio rerio)发育毒性及基因组DNA甲基化影响的研究[J]. 东北师大学报: 自然科学版, 2016, 48(3): 167-173 Chen H S, Sheng L X, Bian H F. Investigation of the effects of developmental toxicity and genomic DNA methylation upon naphthalene exposure in zebrafish (Danio rerio) [J]. Journal of Northeast Normal University : Natural Science Edition, 2016, 48(3): 167-173 (in Chinese)
张恂, 张楠, 朱丹. 斑马鱼在血液系统研究中的应用[J]. 中央民族大学学报: 自然科学版, 2020, 29(4): 67-72 Zhang X, Zhang N, Zhu D. Application of zebrafish in hematological system investigation [J]. Journal of Minzu University of China: Natural Sciences Edition, 2020, 29(4): 67-72 (in Chinese)
Koenig J A, Dao T L, Kan R K, et al. Zebrafish as a model for acetylcholinesterase-inhibiting organophosphorus agent exposure and oxime reactivation [J]. Annals of the New York Academy of Sciences, 2016, 1374(1): 68-77 张棋麟, 江宇航, 董志祥, 等. 汞暴露对斑马鱼肠道菌群结构和肝脏抗氧化指标的影响[J]. 生态环境学报, 2020, 29(8): 1645-1653 Zhang Q L, Jiang Y H, Dong Z X, et al. Effects of mercury exposure on intestinal flora structure and liver antioxidant ability in adult zebrafish [J]. Ecology and Environmental Sciences, 2020, 29(8): 1645-1653 (in Chinese)
Horzmann K A, Freeman J L. Making waves: New developments in toxicology with the zebrafish [J]. Toxicological Sciences: An Official Journal of the Society of Toxicology, 2018, 163(1): 5-12 Cachat J, Stewart A, Utterback E, et al. Three-dimensional neurophenotyping of adult zebrafish behavior [J]. PLoS One, 2011, 6(3): e17597 Singh S K, Sundaram C S, Shanbhag S, et al. Proteomic profile of zebrafish brain based on two-dimensional gel electrophoresis matrix-assisted laser desorption/ionization MS/MS analysis [J]. Zebrafish, 2010, 7(2): 169-177 Garcia G R, Noyes P D, Tanguay R L. Advancements in zebrafish applications for 21st Century toxicology [J]. Pharmacology & Therapeutics, 2016, 161: 11-21 Yang J, Meng Z J, Liu Q, et al. Performing DNA nanotechnology operations on a zebrafish [J]. Chemical Science, 2018, 9(36): 7271-7276 -
点击查看大图
计量
- 文章访问数: 3303
- HTML全文浏览数: 3303
- PDF下载数: 91
- 施引文献: 0
下载:
