冬季新鲜果蔬中细菌分布特征及致病菌耐药性研究
Bacterial Profile and Antibiotic Resistance of Pathogenic Bacteria in Fresh Fruits and Vegetables in Winter
-
摘要: 抗生素在医疗卫生、畜牧养殖等领域发挥巨大作用的同时，其引发的细菌耐药性问题也受到社会各界的广泛关注。目前，食品安全问题日益突出，细菌感染性疾病逐渐增加，针对食品中细菌的分布特征及其对抗生素耐药性状况的研究显得尤为重要。基于微生物培养与抗生素敏感性实验研究了在华北地区冬季购买的6种新鲜果蔬中细菌的分布特征及其对抗生素的耐药性情况。结果表明，6种新鲜果蔬中共鉴定出131株细菌，其中检出益生菌15株，检出率为11.5%，检出条件致病菌80株，检出率为61.1%。检出条件致病菌类型主要包括蜡样芽孢杆菌（Bacillus cereus）、大肠杆菌（Escherichia coli）和恶臭假单胞菌（Pseudomonas putida）等。药敏实验分析表明，检出的条件致病菌对庆大霉素和环丙沙星均敏感，对磺胺甲恶唑和氨苄青霉素表现出高度耐药。本文揭示了新鲜果蔬中抗生素耐药菌的流行情况及肠道菌群中抗生素耐药菌的可能来源，有助于为后续耐药菌的防治及正确评估人体健康风险提供理论依据。Abstract: Antibiotics has brought great convenience to various fields, including medicine and animal husbandry. However, problems caused by its bacterial resistance has attracted wide attentions from every social community. Currently, food safety problems are increasingly prominent, and bacterial infectious diseases are gradually increasing. It is particularly important to study the distribution characteristics of bacteria in food and their resistance to antibiotics. Based on microbial culture enumeration and antibiotic susceptibility test, we detect the prevalence of bacteria and their resistance to antibiotics in 6 kinds of fresh fruits and vegetables collected in winter in North China. The results showed that a total of 131 strains of bacteria were detected. 15 strains of probiotics were detected, and the detection rate was 11.5%. 80 strains of opportunistic pathogens were detected, with a detection rate of 61.1%. The main types of opportunistic pathogen detected mainly include Bacillus cereus, Escherichia coli, Pseudomonas putida, etc. The antibiotic susceptibility test analysis showed that the opportunistic pathogens were sensitive to gentamicin and ciprofloxacin, and displayed high resistance to sulfamethoxazole and ampicillin. This study revealed the prevalence of antibiotic resistance bacteria in fresh fruits and vegetables, and the possible sources of antibiotic resistance bacteria in the gut microbiota, which provides theoretical basis for the subsequent prevention and control of antibiotic resistance bacteria and the correct assessment of human health risks.
-
-
le Page G, Gunnarsson L, Snape J, et al. Integrating human and environmental health in antibiotic risk assessment:A critical analysis of protection goals, species sensitivity and antimicrobial resistance[J]. Environment International, 2017, 109:155-169 李鹏, 谭璐, 李林云, 等. 机舱空气环境耐药基因及耐药细菌的污染特征研究[J]. 生态毒理学报, 2019, 14(3):130-138 Li P, Tan L, Li L Y, et al. Characteristics of antibiotic resistant genes and resistant bacteria in the air environment of cabin[J]. Asian Journal of Ecotoxicology, 2019, 14(3):130-138(in Chinese)
Zhang K, Xin R, Zhao Z, et al. Antibiotic resistance genes in drinking water of China:Occurrence, distribution and influencing factors[J]. Ecotoxicology and Environmental Safety, 2020, 188:109837 Ahmed W, Zhang Q, Lobos A, et al. Precipitation influences pathogenic bacteria and antibiotic resistance gene abundance in storm drain outfalls in coastal sub-tropical waters[J]. Environment International, 2018, 116:308-318 段宇婧, 吴新颜, 陈则友, 等. 人体肠道耐药基因组的研究进展[J]. 生态毒理学报, 2020, 15(4):1-10 Duan Y J, Wu X Y, Chen Z Y, et al. Advances in human gut resistome[J]. Asian Journal of Ecotoxicology, 2020, 15(4):1-10(in Chinese)
Zhao X H, Lin C W, Wang J, et al. Advances in rapid detection methods for foodborne pathogens[J]. Journal of Microbiology and Biotechnology, 2014, 24(3):297-312 Berg G, Erlacher A, Smalla K, et al. Vegetable microbiomes:Is there a connection among opportunistic infections, human health and our gut feeling?[J]. Microbial Biotechnology, 2014, 7(6):487-495 European Food Safety Authority, European Centre for Disease Prevention and Control. The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2016[J]. EFSA Journal European Food Safety Authority, 2017, 15(12):e05077 Liao X Y, Ma Y N, Daliri E B M, et al. Interplay of antibiotic resistance and food-associated stress tolerance in foodborne pathogens[J]. Trends in Food Science & Technology, 2020, 95:97-106 Liu C, Huang H, Zhou Q, et al. Antibacterial and antibiotic synergistic activities of the extract from Pithecellobium clypearia against clinically important multidrug-resistant gram-negative bacteria[J]. European Journal of Integrative Medicine, 2019, 32:100999 Kim N H, Yun A R, Rhee M S. Prevalence and classification of toxigenic Staphylococcus aureus isolated from refrigerated ready-to-eat foods (Sushi, Kimbab and California rolls) in Korea[J]. Journal of Applied Microbiology, 2011, 111(6):1456-1464 Turnbaugh P J, Ley R E, Mahowald M A, et al. An obesity-associated gut microbiome with increased capacity for energy harvest[J]. Nature, 2006, 444(7122):1027-1031 Qin J, Li Y, Cai Z, et al. A metagenome-wide association study of gut microbiota in type 2 diabetes[J]. Nature, 2012, 490(7418):55-60 Knip M, Siljander H. The role of the intestinal microbiota in type 1 diabetes mellitus[J]. Nature Reviews Endocrinology, 2016, 12(3):154-167 Zhang X, Zhang D, Jia H, et al. The oral and gut microbiomes are perturbed in rheumatoid arthritis and partly normalized after treatment[J]. Nature Medicine, 2015, 21(7):895-905 Rooks M G, Garrett W S. Gut microbiota, metabolites and host immunity[J]. Nature Reviews Immunology, 2016, 16(6):341-352 Oliver S P, Jayarao B M, Almeida R A. Foodborne pathogens in milk and the dairy farm environment:Food safety and public health implications[J]. Foodborne Pathogens and Disease, 2005, 2(2):115-129 Law J W F, Letchumanan V, Chan K G, et al. Insights into Detection and Identification of Foodborne Pathogens[M]//Foodborne Pathogens and Antibiotic Resistance. Hoboken, NJ, USA:John Wiley & Sons, Inc., 2017:153-201 Berger C N, Sodha S V, Shaw R K, et al. Fresh fruit and vegetables as vehicles for the transmission of human pathogens[J]. Environmental Microbiology, 2010, 12(9):2385-2397 Karch H, Denamur E, Dobrindt U, et al. The enemy within us:Lessons from the 2011 European Escherichia coli O104:H4 outbreak[J]. EMBO Molecular Medicine, 2012, 4(9):841-848 Brandl M T. Fitness of human enteric pathogens on plants and implications for food safety[J]. Annual Review of Phytopathology, 2006, 44:367-392 Maurice Bilung L, Sin Chai L, Tahar A S, et al. Prevalence, genetic heterogeneity, and antibiotic resistance profile of Listeria spp. and Listeria monocytogenes at farm level:A highlight of ERIC- and BOX-PCR to reveal genetic diversity[J]. BioMed Research International, 2018, 2018:3067494 Cerqueira F, Matamoros V, Bayona J, et al. Antibiotic resistance genes distribution in microbiomes from the soil-plant-fruit continuum in commercial Lycopersicon esculentum fields under different agricultural practices[J]. The Science of the Total Environment, 2019, 652:660-670 He L Y, Ying G G, Liu Y S, et al. Discharge of swine wastes risks water quality and food safety:Antibiotics and antibiotic resistance genes from swine sources to the receiving environments[J]. Environment International, 2016, 92-93:210-219 Cerqueira F, Matamoros V, Bayona J, et al. Distribution of antibiotic resistance genes in soils and crops. A field study in legume plants (Vicia faba L.) grown under different watering regimes[J]. Environmental Research, 2019, 170:16-25 Korir R C, Parveen S, Hashem F, et al. Microbiological quality of fresh produce obtained from retail stores on the Eastern Shore of Maryland, United States of America[J]. Food Microbiology, 2016, 56:29-34 Zhao Y H, Wang Q, Chen Z Y, et al. Significant higher airborne antibiotic resistance genes and the associated inhalation risk in the indoor than the outdoor[J]. Environmental Pollution, 2021, 268:115620 Rodloff A, Bauer T, Ewig S, et al. Susceptible, intermediate, and resistant:The intensity of antibiotic action[J]. Deutsches Arzteblatt International, 2008, 105(39):657-662 Wiegand I, Hilpert K, Hancock R E W. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances[J]. Nature Protocols, 2008, 3(2):163-175 Bretonnière C, Maitte A, Caillon J, et al. MIC score, a new tool to compare bacterial susceptibility to antibiotics application to the comparison of susceptibility to different penems of clinical strains of Pseudomonas aeruginosa[J]. The Journal of Antibiotics, 2016, 69(11):806-810 Bonos E, Giannenas I, Sidiropoulou E, et al. Effect of Bacillus pumilus supplementation on performance, intestinal morphology, gut microflora and meat quality of broilers fed different energy concentrations[J]. Animal Feed Science and Technology, 2021, 274:114859 Abd-El-Kareem F, Elshahawy I E, Abd-Elgawad M M M. Application of Bacillus pumilus isolates for management of black rot disease in strawberry[J]. Egyptian Journal of Biological Pest Control, 2021, 31:25 Punja Z K, Tirajoh A, Collyer D, et al. Efficacy of Bacillus subtilis strain QST 713(Rhapsody) against four major diseases of greenhouse cucumbers[J]. Crop Protection, 2019, 124:104845 Zhao Y Y, Bi J F, Yi J Y, et al. Dose-dependent effects of apple pectin on alleviating high fat-induced obesity modulated by gut microbiota and SCFAs[J]. Food Science and Human Wellness, 2022, 11(1):143-154 Loncarevic S, Johannessen G S, Rørvik L M. Bacteriological quality of organically grown leaf lettuce in Norway[J]. Letters in Applied Microbiology, 2005, 41(2):186-189 Yu K, Newman M C, Archbold D D, et al. Survival of Escherichia coli O157:H7 on strawberry fruit and reduction of the pathogen population by chemical agents[J]. Journal of Food Protection, 2001, 64(9):1334-1340 Krumperman P H. Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of fecal contamination of foods[J]. Applied and Environmental Microbiology, 1983, 46(1):165-170 蒲承君, 吕明环, 曾钰涵, 等. 鸡、猪粪便源大肠杆菌对4种抗生素耐药性的比较[J]. 生态毒理学报, 2017, 12(1):141-147 Pu C J, Lv M H, Zeng Y H, et al. Drug resistance to four antibiotics of Escherichia coli in chicken and pig feces[J]. Asian Journal of Ecotoxicology, 2017, 12(1):141-147(in Chinese)
MacDonald K L. The emergence of Escherichia coli O157:H7 infection in the United States[J]. JAMA, 1993, 269(17):2264 Cooley M, Carychao D, Crawford-Miksza L, et al. Incidence and tracking of Escherichia coli O157:H7 in a major produce production region in California[J]. PLoS One, 2007, 2(11):e1159 Reddy S P, Wang H, Adams J K, et al. Prevalence and characteristics of Salmonella serotypes isolated from fresh produce marketed in the United States[J]. Journal of Food Protection, 2016, 79(1):6-16 Chua P T C, Dykes G A. Attachment of foodborne pathogens to banana (Musa sp.) leaves[J]. Food Control, 2013, 32(2):549-551 Yanagida T, Shimizu N, Kimura T. Extraction of wax and functional compounds from fresh and dry banana leaves[J]. Japan Journal of Food Engineering, 2005, 6(1):79-87 Talley J L, Wayadande A C, Wasala L P, et al. Association of Escherichia coli O157:H7 with filth flies (Muscidae and Calliphoridae) captured in leafy greens fields and experimental transmission of E. coli O157:H7 to spinach leaves by house flies (Diptera:Muscidae)[J]. Journal of Food Protection, 2009, 72(7):1547-1552 Pepper I L, Brooks J P, Gerba C P. Antibiotic resistant bacteria in municipal wastes:Is there reason for concern?[J]. Environmental Science & Technology, 2018, 52(7):3949-3959 Li D L, Zhang H C, Chang F Q, et al. Distribution and health-ecological risk assessment of heavy metals:An endemic disease case study in southwestern China[J]. Environmental Science and Pollution Research International, 2022, 29(3):4260-4275 迟荪琳, 王卫中, 徐卫红, 等. 四环素类抗生素对不同蔬菜生长的影响及其富集转运特征[J]. 环境科学, 2018, 39(2):935-943 Chi S L, Wang W Z, Xu W H, et al. Effects of tetracycline antibiotics on growth and characteristics of enrichment and transformation in two vegetables[J]. Environmental Science, 2018, 39(2):935-943(in Chinese)
邵振鲁, 李厚禹, 李晓晨, 等. 农村固体废弃物中抗生素及耐药基因的赋存及风险管理[J]. 生态毒理学报, 2020, 15(4):112-122 Shao Z L, Li H Y, Li X C, et al. The occurrence and risk management of antibiotics and antibiotic resistant genes in rural solid waste[J]. Asian Journal of Ecotoxicology, 2020, 15(4):112-122(in Chinese)
Sazykin I S, Khmelevtsova L E, Seliverstova E Y, et al. Effect of antibiotics used in animal husbandry on the distribution of bacterial drug resistance (review)[J]. Applied Biochemistry and Microbiology, 2021, 57(1):20-30 Lee K, Kim D W, Lee D H, et al. Mobile resistome of human gut and pathogen drives anthropogenic bloom of antibiotic resistance[J]. Microbiome, 2020, 8(1):2 -
点击查看大图
计量
- 文章访问数: 1931
- HTML全文浏览数: 1931
- PDF下载数: 110
- 施引文献: 0
下载:
