基于配体络合常数的毒代-毒效动力学(TK-TD)模型预测不同pH下镉和铅在斑马鱼幼鱼体内的积累和毒性

高永飞; 何安

doi:10.7524/AJE.1673-5897.20230303001

基于配体络合常数的毒代-毒效动力学(TK-TD)模型预测不同pH下镉和铅在斑马鱼幼鱼体内的积累和毒性

高永飞^1,2, ,,
何安³

1. 太原理工大学生态学学院，太原 030024;
2. 污染环境修复与生态健康教育部重点实验室，浙江大学环境与资源学院，杭州 310058;
3. 南开大学环境科学与工程学院，天津 300071

作者简介: 高永飞(1986-),男,博士,讲师,研究方向为环境毒理学,E-mail:gaoyongfei@tyut.edu.cn

通讯作者: 高永飞,E-mail:gaoyongfei@tyut.edu.cn;

基金项目:
山西省高等学校科技创新项目(2022L067)；中央高校基本科研业务费专项资金(226-2023-00077)；污染环境修复与生态健康教育部重点实验室开放基金(EREH202204)
中图分类号: X171.5

Prediction of Accumulation and Toxicity of Cadmium and Lead in Zebrafish Larvae at Different pH Using A Toxicokinetic-toxicodynamic (TK-TD) Model Based on Ligand Complex Constant

Gao Yongfei^1,2, ,,
He An³

1. College of Ecology, Taiyuan University of Technology, Taiyuan 030024, China;
2. Key Laboratory of Environment Remediation and Ecological Health, Ministry of Education, College of Environmental Resource Sciences, Zhejiang University, Hangzhou 310058, China;
3. College of Environmental Science and Technology, Nankai University, Tianjin 300071, China

Corresponding author: Gao Yongfei, gaoyongfei@tyut.edu.cn ;

Fund Project:

摘要: 复杂多变的水化学条件影响重金属生物有效性和毒性，进一步影响水质基准的制定，需要建立既考虑水化学条件又考虑时间过程的毒代动力学-毒效动力学(toxicokinetics-toxicodynamics, TK-TD)模型去实时地模拟金属的生物蓄积性及产生的毒性。本研究将生物配体模型(biotic ligand model, BLM)中氢离子与配体络合常数(K_HBL)引入TK-TD模型，尝试建立预测水环境不同pH条件下金属毒性的理论模型框架，分别预测镉(Cd)和铅(Pb)在染毒溶液pH为4.5、5.5和6.5下在斑马鱼幼鱼体内的积累和急性毒性，并验证该模型框架的有效性和合理性。结果表明，Pb的最大吸收速率(J_max)比Cd大约3倍。Cd的致死速率(k_k)是Pb的4倍。Cd和Pb的安全阈值(threshold)之间相差30倍。染毒溶液中H⁺浓度增加可显著抑制Cd和Pb在斑马鱼幼鱼体内的累积量。基于K_HBL的TK-TD模型可以较好地预测染毒溶液不同pH(pH=4.5、5.5和6.5)条件下，非必需元素Cd和Pb在斑马鱼幼鱼体内的累积量及产生的毒性。
- 镉 /
- 铅 /
- 氢离子 /
- 毒代动力学 /
- 毒效动力学
Abstract: The complex and ever-changing hydro-chemical conditions affect the bioavailability and toxicity of heavy metals, which further affects the formulation of water quality standards. There is a high demand for developing a toxicokinetic-toxicodynamic (TK-TD) model that considers both hydro-chemical conditions and time processes to simulate the bioaccumulation and toxicity of heavy metals in real-time. This study attempts to establish a theoretical model framework for predicting the toxicity of heavy metals under different pH conditions in the aquatic environment. Additionally, the present work involves the introduction of hydrogen ion-ligand complex constant (K_HBL) in the biotic ligand model (BLM) into a TK-TD model, by predicting the accumulation and acute toxicity of cadmium (Cd) and lead (Pb) in zebrafish (Danio rerio) larvae that are exposed to the aquatic environment of various pH levels (4.5, 5.5, and 6.5). Also, the verification of the effectiveness and rationality of the corresponding model frameworks was carried out in this work. Results showed that the maximum uptake rate (J_max) of Pb was three times higher than that of Cd. The killing rate k_k of Pb is four times lower than that of Cd in the zebrafish larvae. The safe threshold variation between Cd and Pb was found to be 30-fold. Further, the increased H⁺ concentration in poisoning solutions can significantly inhibit the accumulation of Cd and Pb in zebrafish larvae. In conclusion, the K_HBL-rened TK-TD model can accurately predict the accumulation and acute toxicity of Cd and Pb in zebrash larvae at pH 4.5, 5.5, and 6.5.
- cadmium /
- lead /
- H⁺ /
- toxicokinetic /
- toxicodynamic

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基于配体络合常数的毒代-毒效动力学(TK-TD)模型预测不同pH下镉和铅在斑马鱼幼鱼体内的积累和毒性

作者简介: 高永飞(1986-),男,博士,讲师,研究方向为环境毒理学,E-mail:gaoyongfei@tyut.edu.cn

通讯作者: 高永飞,E-mail:gaoyongfei@tyut.edu.cn;

Prediction of Accumulation and Toxicity of Cadmium and Lead in Zebrafish Larvae at Different pH Using A Toxicokinetic-toxicodynamic (TK-TD) Model Based on Ligand Complex Constant

Corresponding author: Gao Yongfei, gaoyongfei@tyut.edu.cn ;

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基于配体络合常数的毒代-毒效动力学(TK-TD)模型预测不同pH下镉和铅在斑马鱼幼鱼体内的积累和毒性

通讯作者: 高永飞,E-mail:gaoyongfei@tyut.edu.cn;

English Abstract

Prediction of Accumulation and Toxicity of Cadmium and Lead in Zebrafish Larvae at Different pH Using A Toxicokinetic-toxicodynamic (TK-TD) Model Based on Ligand Complex Constant

Corresponding author: Gao Yongfei, gaoyongfei@tyut.edu.cn ;

全文HTML

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基于配体络合常数的毒代-毒效动力学(TK-TD)模型预测不同pH下镉和铅在斑马鱼幼鱼体内的积累和毒性

作者简介: 高永飞(1986-),男,博士,讲师,研究方向为环境毒理学,E-mail:gaoyongfei@tyut.edu.cn

通讯作者: 高永飞,E-mail:gaoyongfei@tyut.edu.cn;

Prediction of Accumulation and Toxicity of Cadmium and Lead in Zebrafish Larvae at Different pH Using A Toxicokinetic-toxicodynamic (TK-TD) Model Based on Ligand Complex Constant

Corresponding author: Gao Yongfei, gaoyongfei@tyut.edu.cn ;

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基于配体络合常数的毒代-毒效动力学(TK-TD)模型预测不同pH下镉和铅在斑马鱼幼鱼体内的积累和毒性

通讯作者: 高永飞,E-mail:gaoyongfei@tyut.edu.cn;

English Abstract

Prediction of Accumulation and Toxicity of Cadmium and Lead in Zebrafish Larvae at Different pH Using A Toxicokinetic-toxicodynamic (TK-TD) Model Based on Ligand Complex Constant

Corresponding author: Gao Yongfei, gaoyongfei@tyut.edu.cn ;

全文HTML

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