二苯甲酮(BP-3)对黄尾蓝魔鱼(Chrysiptera parasema)的生态毒理效应
Ecotoxicological Effects of Benzophenone-3 Exposure on Liver of Chrysiptera parasema
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摘要: 紫外线吸收剂已成为一类新型污染物，对珊瑚礁生态系统具有潜在影响。本研究中，将成年黄尾蓝魔鱼分为溶剂对照组CK （0.1%的乙醇）、1 μg·L-1组和10 μg·L-1组，于二苯甲酮（benzophenone-3，BP-3）中暴露7 d，取肝脏进行组织学分析，并检测肝脏中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、脂质过氧化物（MDA）的含量变化，以及抗氧化酶基因的表达，以揭示BP-3对黄尾蓝魔鱼肝脏生态毒理效应。结果表明，暴露7 d后，10 μg·L-1组黄尾蓝魔鱼肝细胞肿大，核偏离和细胞核固缩。从抗氧化指标来看，BP-3胁迫后，对肝脏抗氧化酶活性有一定明显的影响。不同浓度BP-3染毒后，SOD酶、CAT酶活性降低，并表现出一定的剂效关系，但与对照组相比没有显著性差异（P>0.05）。MDA含量胁迫后出现先增后降的趋势，低浓度（1 μg·L-1） BP-3胁迫后，MDA含量显著上升（P<0.01），随着染毒浓度增加含量有所下降。不同基因的表达水平对BP-3胁迫的响应程度有差异，基因bdh2的表达量胁迫以后呈现上升的趋势，SCO1基因的表达量胁迫后出现下降的趋势，均接近显著性水平（P=0.068）；CYP27B基因的表达量与对照组相比在1 μg·L-1（P<0.05）和10 μg·L-1（P<0.01）时显著下降，表现出明显的剂效关系。FTHFD和NADH基因表达量随染毒浓度的上升而变化，但与对照组相比没有差异。综上，MDA含量和CYP27B基因表达水平可以作为敏感的生物标志物来反映BP-3胁迫对黄尾蓝魔鱼的生态毒性效应。本研究揭示了BP-3对黄尾蓝魔鱼肝脏生态毒理效应，为探讨紫外线吸收剂对海洋生态系统的潜在毒性提供了科学依据。Abstract: UV filters has become one kind of emerging contaminants with potential impacts on coral reef ecosystem. At present study, the toxicological effects of adult Chrysiptera parasema was exposed to benzophenone-3 (BP-3), which has been evaluated based on the liver histology and antioxidant defense system. Chrysiptera parasema was exposed to the solvent control groups (CK, 0.1% ethanol), 1 μg·L-1 and 10 μg·L-1 of BP-3 for 7 d. The results showed the hepatocytes of enlarged Chrysiptera parasema, nuclear deviation and nucleus fixation after 7 d exposure at the 10 μg·L-1 group. Moreover, BP-3 exposure showed significant effects on the antioxidant enzyme activities of the liver. The superoxide dismutase (SOD) and catalase (CAT) activities decreased at different exposure concentrations of BP-3, and there exists an obvious dose-response relationship. However, there was no significant difference between the control group and other treatment groups (P>0.05) for SOD and CAT activities. The malondialdehyde (MDA) content increased significantly (P<0.01) at the concentration of 1 μg·L-1, and decreased at the concentration of 10 μg·L-1. The expression levels of different genes were different in response to BP-3 stress, with the expression of bdh2 gene showing an increasing trend and SCO1 gene showing a decreasing trend after BP-3 exposure. The expression of CYP27B gene decreased significantly at 1 μg·L-1 (P<0.05) and 10 μg·L-1 (P<0.01) groups compared with the control group, showing a significant dose-response relationship. In conclusion, MDA content and CYP27B gene expression level could be considered as sensitive biomarkers to reflect the ecotoxicological effects of BP-3 on Chrysiptera parasema. This study provides essential information for exploring the potential toxicological effects of BP-type UV filters on marine ecosystems.
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Key words:
- benzophenone-3 /
- Chrysiptera parasema /
- liver /
- oxidative damage
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Tang Z W, Han X, Li G H, et al. Occurrence, distribution and ecological risk of ultraviolet absorbents in water and sediment from Lake Chaohu and its inflowing rivers, China[J]. Ecotoxicology and Environmental Safety, 2018, 164:540-547 Sánchez Rodríguez A, Rodrigo Sanz M, Betancort Rodríguez J R. Occurrence of eight UV filters in beaches of Gran Canaria (Canary Islands). An approach to environmental risk assessment[J]. Chemosphere, 2015, 131:85-90 吴钟解, 陈石泉, 陈敏, 等. 海南岛造礁石珊瑚资源初步调查与分析[J]. 海洋湖沼通报, 2013(2):44-50 Wu Z J, Chen S Q, Chen M, et al. Preliminary survey and analysis of the resources of hermatypic corals in Hainan Island[J]. Transactions of Oceanology and Limnology, 2013 (2):44-50(in Chinese)
Liu H, Sun P, Liu H X, et al. Hepatic oxidative stress biomarker responses in freshwater fish Carassius auratus exposed to four benzophenone UV filters[J]. Ecotoxicology and Environmental Safety, 2015, 119:116-122 Liu H, Sun P, Liu H X, et al. Hepatic oxidative stress biomarker responses in freshwater fish Carassius auratus exposed to four benzophenone UV filters[J]. Ecotoxicology and Environmental Safety, 2015, 119:116-122 He L, He T, Farrar S, et al. Antioxidants maintain cellular redox homeostasis by elimination of reactive oxygen species[J]. Cellular Physiology and Biochemistry:International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology, 2017, 44(2):532-553 Farombi E O, Adelowo O A, Ajimoko Y R. Biomarkers of oxidative stress and heavy metal levels as indicators of environmental pollution in African cat fish (Clarias gariepinus) from Nigeria Ogun River[J]. International Journal of Environmental Research and Public Health, 2007, 4(2):158-165 Huang X X, Li Y Y, Wang T T, et al. Evaluation of the oxidative stress status in zebrafish (Danio rerio) liver induced by three typical organic UV filters (BP-4, PABA and PBSA)[J]. International Journal of Environmental Research and Public Health, 2020, 17(2):651 Ma B N, Lu G H, Yang H H, et al. The effects of dissolved organic matter and feeding on bioconcentration and oxidative stress of ethylhexyl dimethyl p-aminobenzoate (OD-PABA) to crucian carp (Carassius auratus)[J]. Environmental Science and Pollution Research International, 2018, 25(7):6558-6569 Rajeshkumar S, Liu Y, Ma J G, et al. Effects of exposure to multiple heavy metals on biochemical and histopathological alterations in common carp, Cyprinus carpio L.[J]. Fish & Shellfish Immunology, 2017, 70:461-472 Tsikas D. Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples:Analytical and biological challenges[J]. Analytical Biochemistry, 2017, 524:13-30 Gaweł S, Wardas M, Niedworok E, et al. Malondialdehyde (MDA) as a lipid peroxidation marker[J]. Wiadomosci Lekarskie, 2004, 57(9-10):453-455 Bilan D S, Lukyanov S A, Belousov V V. Genetically encoded fluorescent redox sensors[J]. Bioorganicheskaia Khimiia, 2015, 41(3):259-274 Yang B, Hou S H, Zhao J J, et al. 3-hydroxy butyrate dehydrogenase 2 deficiency aggravates systemic lupus erythematosus progression in a mouse model by promoting CD40 ligand demethylation[J]. Bioengineered, 2022, 13(2):2685-2695 Liu J Z, Hu Y L, Feng Y, et al. BDH2 triggers ROS-induced cell death and autophagy by promoting Nrf2 ubiquitination in gastric cancer[J]. Journal of Experimental & Clinical Cancer Research, 2020, 39(1):123 Niimura Y. The NADH oxidase-prx system in Amphibacillus xylanus[J]. Sub-Cellular Biochemistry, 2007, 44:195-205 Jonas M I, Kuryłowicz A, Bartoszewicz Z, et al. Vitamin D receptor gene expression in adipose tissue of obese individuals is regulated by miRNA and correlates with the pro-inflammatory cytokine level[J]. International Journal of Molecular Sciences, 2019, 20(21):E5272 -
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