诺氟沙星对刺参肠道菌群结构与功能特征的影响
Effects of Norfloxacin on Structure and Function of Gut Bacterial Community of Apostichopus japonicus
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摘要: 近年来，诺氟沙星（norfloxacin，NOR）在水环境中被广泛检出。为探究NOR对棘皮动物的毒性效应，选取刺参（Apostichopus japonicus）为研究对象，将其暴露于0 mg·L-1、1.5×10-1 mg·L-1和150 mg·L-1浓度诺氟沙星中56 d，研究NOR暴露对刺参肠道菌群结构及功能的影响。结果显示：刺参肠道核心菌群为变形菌门（Proteobacteria）和疣微菌门（Verrucomicrobia）。与对照组相比，随着环境中NOR暴露浓度的增加，刺参肠道菌群逐渐被罗尔斯通氏菌（Ralstonia）取代，150 mg·L-1 NOR处理组罗尔斯通氏菌相对丰度占比高达79.82%，呈现出肠道菌群单一化的现象。基于京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）代谢通路数据库注释，NOR暴露组肠道菌群mRNA翻译、蛋白质复制合成及修复、多糖合成及代谢通路丰度显著上调（P<0.05），而膜运输、氨基酸代谢通路丰度显著下调（P<0.05）。综上可知，水体中NOR影响了刺参肠道菌群结构与功能。本研究旨为水体抗生素生态风险评估提供一定的理论依据。Abstract: In recent years, norfloxacin (NOR) has been widely detected in the aquatic environment. In order to investigate the toxic effects of NOR on echinoderm, the Apostichopus japonicus was selected as the research object and exposed to 0 mg·L-1, 1.5×10-1 mg·L-1 and 150 mg·L-1 concentrations of NOR for 56 days. The effects of NOR exposure on the structure and function of the gut bacterial community of A. japonicus were investigated. The results showed that the core gut bacterial community of A. japonicus were Proteobacteria and Verrucomicrobia. Compared with the control group, with the increase of NOR exposure concentration in the environment, the gut bacterial community of A. japonicus was gradually replaced by Ralstonia, and the relative abundance of Ralstonia in the 150 mg·L-1 NOR-treated group accounted for as high as 79.82%, which showed the phenomenon of homogenization of the gut bacterial community. Based on the KEGG (Kyoto Encyclopedia of Genes and Genomes) metabolic pathway database annotation, the abundance of mRNA translation, protein replication synthesis and repair, polysaccharide synthesis and metabolic pathways was significantly up-regulated in thegut bacterial community of the NOR-exposed group (P<0.05), whereas the abundance of membrane transport and amino acid metabolic pathways was significantly down-regulated (P<0.05). In conclusion, NOR in the water affected the structure and function of the gut bacterial community of A. japonicus. This study aims to provide some theoretical basis for the ecological risk assessment of antibiotics in water.
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